Navigation Links
Nanoscale gene 'ignition switch' may help spot and treat cancer
Date:12/15/2010

In a proof of principal study in mice, scientists at Johns Hopkins and the Virginia Commonwealth University (VCU) have shown that a set of genetic instructions encased in a nanoparticle can be used as an "ignition switch" to rev up gene activity that aids cancer detection and treatment.

The switch, called a promoter, is a set of chemical letters that interacts with DNA to turn on gene activity. In this case, the scientists used a promoter called PEG-Prom, cloned by VCU researcher Paul Fisher, Ph.D. PEG-Prom is activated only when inside cancer cells, not in normal ones.

"With current imaging devices like CT and PET, we can tell if something is wrong in a patient, but we don't have definitive tools to distinguish cancer from inflammation or infection," says Martin Pomper, M.D., Ph.D., professor of radiology at Johns Hopkins. "It generally takes at least one month after giving patients certain cancer treatments before existing imaging tools can measure the patient's response to the therapy."

To differentiate cancer cells from normal cells, Johns Hopkins scientists connected PEG-Prom to either a gene that produces firefly luciferase, the substance that make fireflies glow, or a gene called HSV1tk, which initiates a chemical reaction with radioactive labels inside the cell that can be detected by imaging devices. Once inside a cancer cell, the PEG-Prom switch is turned on, and it activates either the luciferase or HSV1tk gene.

Then, they stuffed the PEG-Prom/gene combination into tiny spheres about 50,000 times smaller than the head of a pin and intravenously injected the nanoparticles into mice with either metastatic breast cancer or melanoma.

The findings, reported in the December 12 online edition of Nature Medicine, reveal a 30-fold difference in identifying cancer cells containing luciferase and normal cells that did not contain the substance. Similar results were observed in cancer cells filled with the radioactive labels and normal ones that were not.

"This type of imaging technique has the potential to add to existing tools with more specificity in identifying the problem," says Pomper.

Pomper says that the technique could likely be used in any cancer, and the nanoparticle and HSV1tk gene used in the current study have been tested previously in clinical studies unrelated to Pomper's work.

In addition to diagnostic and monitoring tools, the technique could be designed to deliver therapies to the heart of cancer cells. One approach, he says is to use radioactive isotopes to make cancer cells radioactive from the inside, instead of delivering radiation to the patient externally.

Still, Pomper says, such a technique would be limited to identifying tumors that are two millimeters or larger, amounting to millions of cells, because current imaging devices cannot detect anything smaller. He also says that certain doses of nanoparticles could be toxic, so his team is conducting tests to find the best nanoparticle.


'/>"/>

Contact: Vanessa Wasta
wasta@jhmi.edu
410-955-1287
Johns Hopkins Medical Institutions
Source:Eurekalert

Related medicine news :

1. Nanoscale stealth probe slides into cell walls seamlessly, say Stanford engineers
2. Cochlear Appoints Switched on Media to Develop its SEO Across the Asia Pacific Region
3. Researchers create drug to keep tumor growth switched off
4. BCS Global Launches PSVN (Public Switched Video Network), an Initiative to Provide a Framework for Inter-Connecting Business to Business Video Networks
5. Daylight-Saving Time Switch May Leave You Sleepy, U-M Physician Says
6. Researchers discover brain tumors grow-or-go switch
7. Discovery of cellular switch may provide new means of triggering cell death, treating disease
8. UBC graduate student finds a start/stop switch for retroviruses
9. Brains master switch is verified by Iowa State University researcher
10. Clue to switch of bladder cancer from locally contained to invasive found by Jefferson scientists
11. Memorys master switch
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... June 27, 2016 , ... ... announced its strategic partnership with Connance, a healthcare industry leader providing predictive ... proven, proprietary technology combine to provide health systems, hospitals and ambulatory surgical ...
(Date:6/26/2016)... ... June 26, 2016 , ... Pixel Film Studios Released ProSlice ... "Film editors can give their videos a whole new perspective by using the ... of Pixel Film Studios. , ProSlice Levels contains over 30 Different presets to ...
(Date:6/25/2016)... ... June 25, 2016 , ... Austin residents seeking Mohs surgery services, can now ... and to Dr. Russell Peckham for medical and surgical dermatology. , Dr. Dorsey brings ... The selective fellowship in Mohs Micrographic Surgery completed by Dr. Dorsey was under the ...
(Date:6/25/2016)... ... 25, 2016 , ... Dr. Calvin Johnson has dedicated his ... implemented orthobiologic procedures as a method for treating his patients. The procedure is ... to perform the treatment. Orthobiologics are substances that orthopaedic surgeons use to help ...
(Date:6/24/2016)... ... 24, 2016 , ... June 19, 2016 is World Sickle Cell Observance Day. ... the benefits of holistic treatments, Serenity Recovery Center of Marne, Michigan, has ... , Sickle Cell Disease (SCD) is a disorder of the red blood cells, which ...
Breaking Medicine News(10 mins):
(Date:6/26/2016)... June 27, 2016  VMS Rehab Systems, Inc. ( ... take whatever measures required to build a strong and ... is currently listed on the OTC Markets-pink current trading ... and CEO, "We are seeing an anomaly in market ... not only by the Company, but shareholders and market ...
(Date:6/24/2016)... SOUTH SAN FRANCISCO, Calif. , June 24, ... GBT ), a biopharmaceutical company developing novel ... with significant unmet needs, today announced the closing ... 6,400,000 shares of common stock, at the public ... the shares in the offering were offered by ...
(Date:6/24/2016)... Va. , June 24, 2016 The ... set of recommendations that would allow biopharmaceutical ... (HCEI) with entities that make formulary and coverage decisions, ... the "value" of new medicines. The recommendations ... does not appear on the drug label, a prohibition ...
Breaking Medicine Technology: