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Nanometer-scale growth of cone cells tracked in living human eye
Date:12/20/2011

WASHINGTON, Dec. 20 -- Humans see color thanks to cone cells, specialized light-sensing neurons located in the retina along the inner surface of the eyeball. The actual light-sensing section of these cells is called the outer segment, which is made up of a series of stacked discs, each about 30 nanometers (billionths of a meter) thick. This appendage goes through daily changes in length. Scientists believe that a better understanding of how and why the outer segment grows and shrinks will help medical researchers identify potential retinal problems. But the methods usually used to image the living human eye are not sensitive enough to measure these miniscule changes. Now, vision scientists at Indiana University in Bloomington have come up with a novel way to make the measurements in a living human retina by using information hidden within a commonly used technique called optical coherence tomography (OCT). They discuss their results in the Optical Society's (OSA (http://www.osa.org)) open-access journal Biomedical Optics Express(http://www.opticsinfobase.org/boe).

To make an OCT scan of the retina, a beam of light is split into two. One beam scatters off the retina while the other is preserved as a reference. The light waves begin in synch, or in phase, with each other; when the beams are reunited, they are out of phase, due to the scattering beam's interactions with retinal cells. Scientists can use this phase information to procure a precise measurement of a sample's position. But since in this case their samples were attached to live subjects, the researchers had to adapt these typical phase techniques to counteract any movements that the subjects' eyes might insert into the data.

Instead of measuring the phase of a single interference pattern, the researchers measured phase differences between patterns originating from two reference points within the r
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Contact: Angela Stark
astark@osa.org
202-416-1443
Optical Society of America
Source:Eurekalert

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