Navigation Links
NIH scientists describe how salmonella bacteria spread in humans
Date:9/30/2010

New findings by National Institutes of Health scientists could explain how Salmonella bacteria, a common cause of food poisoning, efficiently spread in people. In a study published this week in the Proceedings of the National Academy of Sciences, researchers describe finding a reservoir of rapidly replicating Salmonella inside epithelial cells. These bacteria are primed to infect other cells and are pushed from the epithelial layer by a new mechanism that frees the Salmonella to infect other cells or be shed into the intestine.

The Centers for Disease Control and Prevention estimate that Salmonella infections sicken 40,000 people each year in the United States, though the actual number of infections is likely much higher because many cases are mild and not diagnosed or reported. Currently, Salmonella is the focus of an ongoing U.S. public health investigation into contaminated chicken eggs.

"Unfortunately, far too many people have experienced the debilitating effects of Salmonella, which cause disease via largely unexplained processes, including overactive inflammatory responses," says Anthony S. Fauci, M.D., director of NIH's National Institute of Allergy and Infectious Diseases (NIAID). "This elegant study provides new insight into the origins of that inflammatory disease process."

While much is known about the human infectious cycle of Salmonella, scientists have yet to understand how the bacteria escape the gut to spread infection. Epithelial cells line the outer and inner surfaces of the body, such as the skin and gut, and form a continuous protective tissue against infection. But Salmonella have learned how to live inside epithelial cells and use them for their benefit. Salmonella protect themselves within special membrane-bound compartments, called vacuoles, inside gut epithelial cells.

Using special high-resolution microscopes to view laboratory-grown human intestinal epithelial cells and laboratory mice infected with Salmonella, an NIAID research group led by Olivia Steele-Mortimer, Ph.D., in collaboration with Bruce Vallance, Ph.D., of the University of British Columbia in Vancouver, discovered a secondary population of Salmonella not confined within a vacuole, but instead moving freely inside the epithelial cells. This reservoir of Salmonella is distinct from vacuolar Salmonella. The bacteria multiply much faster; they have long tail-like projections, called flagella, used to move; and they exhibit a needle complex they use to pierce cells and inject their proteins. With these attributes, this population of Salmonella is genetically programmed to invade new cells.

The scientists observed that epithelial cells containing the hyper-replicating, invasive Salmonella are eventually pushed out of the intestinal tissue into the gut cavity, setting the Salmonella free. The mechanism used to push these Salmonella-infected cells into the body cavity resembles the natural mechanism humans use to shed dying or dead epithelial cells from their gut. The scientists believe that Salmonella have hijacked this mechanism to facilitate their own escape.

The human immune system, however, also senses that these are not normal, dying cells in the gut and triggers a response that includes release of interleukin-18, a small protein that sets off an inflammation cascade. Interleukin-18 also is prominent in chronic intestinal inflammation associated with autoimmune disorders, such as inflammatory bowel disease. The effects of interleukin-18 release provide an explanation for the acute intestinal inflammation associated with Salmonella infections.

The scientists hope their research leads to a treatment that prevents the spread of infection. They are focusing on how this specialized population of Salmonella escapes from its membrane-bound compartment to multiply and swim freely in the cell.


'/>"/>

Contact: Ken Pekoc
kpekoc@niaid.nih.gov
301-402-1663
NIH/National Institute of Allergy and Infectious Diseases
Source:Eurekalert

Related medicine news :

1. UNC scientists receive grant to develop nanotechnology for pancreatic cancer diagnosis and treatment
2. Unlocking the secret of beauty: Scientists discover the complexities of attractive female bodies
3. Symposium in Washington with world-leading scientists
4. Researcher seeks to create citizen scientists to help in response to environmental disasters
5. UC Davis scientists find link between arthritis pain reliever and cardiovascular events
6. Leading brain scientists meet in Milwaukee to examine how to uncover the brains secrets
7. NYU Langone scientists find key pathway implicated in progression of childhood cancer
8. Welsh scientists clone human virus
9. Scripps Research scientists solve long-standing mystery of protein quality control mechanism
10. Scientists identify molecular gatekeeper of arthritis
11. Scientists make leap forward in early detection for Alzheimers and cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/9/2016)... ... February 09, 2016 , ... Center for Autism ... diagnosed with autism spectrum disorder (ASD). Beginning Jan. 1, 2016, Medicaid is covering ... analysis (ABA) is key to providing effective treatment for individuals with ASD. Now, ...
(Date:2/9/2016)... ... February 09, 2016 , ... A ... fee schedule rates and Medicare rates for a variety of medical services in ... Evaluation of the 2015 Fee Schedule Rates in Illinois, are professional ...
(Date:2/9/2016)... ... 2016 , ... Establishment Labs, a global breast implant and medical technology company, ... of Directors. , “We are honored to welcome David to our Board ... Jose Chacon Quiros, CEO of Establishment Labs. "David’s knowledge and experience will be invaluable ...
(Date:2/9/2016)... ... 2016 , ... Dr. Rassouli, cosmetic dentist in Orange County , is ... popular cosmetic procedures in dentistry today, but the cost often leads to patients looking ... of teeth whitening-related damage. For a limited time, Dr. Rassouli is offering this special ...
(Date:2/9/2016)... ... February 09, 2016 , ... Two renowned photographers, Robert Caplin ... photographic adventure on the island, June 14-19, 2016, hosted by Four Seasons Resort Maui ... , After a successful debut in 2015, the Maui Photo Expedition workshop ...
Breaking Medicine News(10 mins):
(Date:2/9/2016)... Mass. , Feb. 9, 2016   AllCare ... The Joint Commission,s Gold Seal of Approval ® ... compliance with its performance standards. The Gold Seal of ... reflects an organization,s commitment to providing safe and effective ... AllCare Plus Pharmacy underwent a rigorous on-site survey ...
(Date:2/9/2016)... 2016 The leader in accelerated orthodontics, OrthoAccel ... is the recipient of the 2015 Townie Choice Award ... II medical device that speeds up orthodontic tooth movement ... often associated with treatment, AcceleDent was selected by orthodontists ... Orthotown survey of the most reliable and ...
(Date:2/9/2016)... Feb. 9, 2016  Johnson & Johnson (NYSE: ... Global Healthcare Conference on Tuesday, Feb. 23, at the ... Caruso , Vice President, Finance & Chief Financial Officer ... represent the Company in a session scheduled at 10:00 ... --> www.investor.jnj.com . --> This webcast ...
Breaking Medicine Technology: