Navigation Links
NIBIB scientists increase imaging efficiency in cell structure studies

Scientists in the National Institute of Biomedical Imaging and Bioengineering (NIBIB) Laboratory of Bioengineering and Physical Science have developed a new technique that allows researchers to visualize fine details of cell structure three-dimensionally in thick sections, thus providing greater insight into how cells are organized and how they function. The work is described in a report published online this week in Nature Methods.

The new electron tomography method, referred to as BF STEM tomography, lets researchers image samples that are more than three times the thickness of typical samples.

Electron tomography is carried out at the nanoscale on individual cells. Conventionally, high-resolution imaging of biological specimens has been accomplished by cutting cells into thin sections (300 nanometers or less) and imaging each section separately. Although reconstructing an entire structure from thin sections is laborious, thin sections are used because images of thicker sections typically are blurred. Serial BF STEM tomography accomplishes the same work using fewer yet thicker specimen sections, leading to faster reconstruction of intact organelles, intracellular pathogens, and even entire mammalian cells.

Drs. Alioscka Sousa, Martin Hohmann-Marriott, Richard Leapman and colleagues in NIBIB, in collaboration with Dr. Joshua Zimmerberg and colleagues in the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), demonstrated feasibility and advantages of BF-STEM tomography in a study of red blood cells infected with Plasmodium falciparum, a parasite that causes malaria. High-resolution 3D reconstructions of entire cells were generated by serially imaging just a few thick sections. The intricate system of red blood cell and parasite membranes, as well as several organelles, can be seen in detail.

"We believe that the new technique, which was conceived by Dr. Sousa on the project team, will lead to improved 3D visualization of larger internal structures in mammalian cells at a nanoscale. And it will complement cryo electron tomography and super-resolution optical imaging approaches," according to Dr. Leapman.

Most high-performance electron microscopes can readily be equipped to utilize the BF STEM tomography approach. "This exciting new method, with its ability to provide nanoscale structural details over three dimensions, has the potential for broad application in cell biology," says NIBIB Director Roderic Pettigrew. "This should open new vistas in the understanding of the interplay between cellular structure and function, and is a great example of NIBIB-supported research that moves medical science forward through technological innovation."


Contact: Cheryl Fee
NIH/National Institute of Biomedical Imaging & Bioengineering

Related medicine news :

1. Scientists Make Sweet Monkey Music
2. Scientists from University of Hawaii at Manoa find genetic marker
3. Nottingham scientists commissioned for urgent swine flu research
4. Ellison Medical Foundation awards more than $1 million to mid-career scientists
5. New Federal Training Grant to Teach Doctors/Scientists Immunology
6. Scientists Spot How Sugar Feeds Tumors
7. Argonne, University of Chicago Scientists Develop Targeted Cancer Treatment Using Nanomaterials
8. MSU scientists: Progesterone leads to inflammation
9. Argonne, University of Chicago scientists develop targeted cancer treatment using nanomaterials
10. Pitt scientists find intrinsic changes in protein shape influence drug binding
11. Hard To Treat Diseases (HTDS.PK) Scientists present encouraging results for the future of Multiple Sclerosis treatment.
Post Your Comments:
(Date:11/28/2015)... CA (PRWEB) , ... November 28, 2015 , ... Pixel ... fully customizable media panels to choose from, the possibilities are endless. Users have full ... more. With the ProPanel: Pulse masking effects, users are sure to get heads to ...
(Date:11/27/2015)... Denver, CO (PRWEB) , ... November 27, 2015 , ... ... U.S. cities are not changing the way that they are handling security in light ... increasing police and security presence in an attempt to stop an attack from reaching ...
(Date:11/27/2015)... ... November 27, 2015 , ... ... toilets were," said an inventor from Hillside, N.J. "Many people catch diseases simply ... so that individuals will always be protected from germs." , He developed the ...
(Date:11/27/2015)... (PRWEB) , ... November 27, 2015 , ... MPWH, the No.1 Herpes-only dating community in ... (see Table 1-1 ). More than 3.7 billion people under the age of ... 1 (HSV-1), according to WHO's first global estimates of HSV-1 infection . , "The ...
(Date:11/27/2015)... ... November 27, 2015 , ... ... Consultants has prided itself for not only fulfilling the needs of advisers and ... at an affordable price and providing top-tier customer service. However, there's always room ...
Breaking Medicine News(10 mins):
(Date:11/26/2015)... , November 26, 2015 ... the addition of the  "2016 Future ... European Therapeutic Drug Monitoring (TDM) Market: ... Intelligence, Emerging Opportunities"  report to their ... has announced the addition of the  ...
(Date:11/26/2015)... 26, 2015 ... of the  "2016 Future Horizons and ... Surface Marker Testing Market: Supplier Shares, ... their offering.  --> ... the  "2016 Future Horizons and Growth ...
(Date:11/25/2015)... N.Y. , Nov. 25, 2015  Linden Care, ... and optimizing treatment outcomes for patients suffering from chronic ... request for a Temporary Restraining Order (TRO) enjoining Express ... the two companies. --> ... pursuing all of its legal options. ...
Breaking Medicine Technology: