Navigation Links
NCI scientists identify targets for melanoma immunotherapy
Date:9/10/2013

PHILADELPHIA Using a highly sensitive technology called NanoString, researchers have identified seven targets that could potentially be used to develop new immunotherapies for patients with metastatic melanoma, according to a study published in Clinical Cancer Research, a journal of the American Association for Cancer Research.

"We identified seven potential candidate genes that deserve further consideration as targets for melanoma immunotherapy," said Richard Morgan, Ph.D., staff scientist at the Tumor Immunology Section of the Center for Cancer Research, National Cancer Institute (NCI), in Bethesda, Md. "We used NanoString technology because it is very robust, yielding quantitative and extremely reproducible results and, in addition, an antigen expression profile can be constructed for a patient from a very small amount of tumor sample, which makes NanoString a better clinical tool."

NanoString technology allows for the simultaneous measurement of multiple genes that are expressed in higher amounts in tumor cells than in normal cells. Researchers isolate genetic material called RNA from tumor samples, use this technology to measure the amount of RNAs present in tumors, and compare them with those present in normal tissues. Unlike many other technologies that can be used for this purpose, NanoString can detect and measure the expression levels of the genes in a single step, avoiding errors.

"Our laboratory has developed a battery of different antigen receptors to target a wide range of antigens, and we can engineer human immune cells to recognize the targets in patients' tumors and kill those cells," said Morgan. "The NCI surgery branch is conducting several clinical trials using this technology to treat a variety of cancers."

In these clinical trials, the NCI researchers are treating patients with adoptive immunotherapy, a type of cancer treatment that involves "teaching" the immune cells of a patient to locate specific targets on tumor cells and kill those cells. Identification of the right targets present in a patient's tumor is, therefore, essential for immunotherapy to be successful.

Morgan and colleagues took metastatic melanoma samples from 59 patients, five established melanoma cell lines, and 31 normal tissue samples to profile the excessively expressed genes using NanoString. The researchers identified 67 genes that can be recognized by immune cells as targets on melanoma cells. They subsequently short-listed seven of these genes as ideal candidates because these genes fit the criteria: they were excessively expressed in melanoma tumors and were absent or at low levels in normal tissues, thus targeting them should cause no or minimal toxicity to nontarget organs.

Of these seven genes, five of them, CSAG2, IL13RA2, MAGEA3, MAGEC2, and PRAME, belong to a family called the cancer-testis genes, and the remaining two, CSPG4 and SOX10, are melanoma-related genes. Further investigation is needed before immune cells engineered to target these markers can be used in patients, according to Morgan.

"This is a good example of how newer technologies like NanoString arm cancer researchers and clinicians with the best gear to make tremendous advancements in cancer research and treatment," said Morgan. His team is currently using this technology to identify immunotherapy targets for pancreatic cancer.


'/>"/>

Contact: Jeremy Moore
jeremy.moore@aacr.org
215-446-7109
American Association for Cancer Research
Source:Eurekalert

Related medicine news :

1. Scientists solving the mystery of human consciousness
2. Scientists uncover multiple faces of deadly breast cancer
3. Scientists identify major source of cells defense against oxidative stress
4. Scientists tailor cell surface targeting system to hit organelle ZIP codes
5. Scientists rewrite rulebook on breast cancer in landmark global study
6. Warwick scientists uncover how checkpoint proteins bind chromosomes
7. NIH scientists link quickly spreading gene to Asian MRSA epidemic
8. Joslin scientists identify important mechanism that affects the aging process
9. Scripps Research scientists show how memory B cells stay in class to fight different infections
10. Scientists Map Melanomas Genome
11. A*STAR scientists discover switch to boost anti-viral response to fight infectious diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/23/2017)... ... January 23, 2017 , ... ... in partnership with the American Society of Anesthesiologists® (ASA®), the Foundation for ... American Academy of Orthopaedic Surgeons (AAOS) and the Cigna Foundation, encourages proposals ...
(Date:1/23/2017)... ... January 23, 2017 , ... The ... at seafoodnutrition.org/programs and seafoodnutrition.org/resources to assist in teaching communities and individuals about ... been developed for use by nutrition educators and influencers within the public ...
(Date:1/22/2017)... ... 22, 2017 , ... Phytocéane invites clients to take an exotic journey deep ... with ZANZIBAR MILKY CREAM. Inspired by the beauty of Zanzibar, a Tanzanian archipelago off ... moisturizing vegetal coral to create this gentle, velvety body cream to envelop the skin ...
(Date:1/21/2017)... , ... January 21, 2017 , ... Caronlab Australia, an ... the January ECRM Trade Show in Hilton Head, SC, where it benefited from outstanding ... the quality of its beauty and wellness products. At this trade show, the company ...
(Date:1/21/2017)... ... 21, 2017 , ... Phytocéane invites you to take an exotic ... the world with ZANZIBAR SHOWER GEL. Inspired by the beauty of Zanzibar, a Tanzanian ... and moisturizing vegetal coral to create this gentle, crystal-clear shower gel to cleanse the ...
Breaking Medicine News(10 mins):
(Date:1/23/2017)... Januar 2017  ResMed (NYSE: RMD ), ... Medical ( Winter Haven, Florida ) gaben ... die Beilegung aller globalen Rechtsstreitigkeiten zwischen den Parteien geeinigt ... ihrer bestehenden Produkte im Tausch gegen Lizenzgeb├╝hren an ResMed ... leisten, um das in Florida ...
(Date:1/23/2017)... Just two weeks remain until legal experts and senior industry ... Central London to discuss the flow of pharmaceutical products ... Parallel Trade 2017 . In the run up to ... is available to read in the event download centre . ... Some of those confirmed include: ...
(Date:1/21/2017)... PUNE, India , January 20, 2017 ... by Type (Descriptive, Prescriptive), Application (Marketing, R&D, Compliance, SCM), Component (Software, ... CRO) - Forecasts to 2021" published by MarketsandMarkets, the market is ... Billion in 2016, at a CAGR of 13.3% during the forecast ... ...
Breaking Medicine Technology: