Navigation Links
Molecules on branched-polymer surfaces can capture rare tumor cells in blood
Date:11/17/2011

The removal of rare tumor cells circulating in the blood might be possible with the use of biomolecules bound to dendrimers, highly branched synthetic polymers, which could efficiently sift and capture the diseased cells, according to new research at the University of Illinois at Chicago.

Dendrimers have been used to encapsulate drug molecules and serve as a delivery vehicle, but in the new study they were employed to capture circulating tumor cells by biomimicry -- using nanotechnology to create artificial surfaces much like those in real cells.

"We want to take advantage of what nature gives us," says Seungpyo Hong, lead researcher of the study, published in the journal Angewandte Chemie. "We want to create new biomimetic surfaces that will allow us to remove damaged cells from the blood."

Hong, assistant professor of biopharmaceutical sciences at UIC, and his coworkers created a highly sensitive surface that enables multivalent binding -- the simultaneous binding of many molecules to multiple receptors in a biological system. The biomimetic surface was created using dendrimers of seventh-generation polyamidoamine, or PAMAM, and the anti-epithelial cell adhesion molecule, or aEpCAM.

In the body, cancer cells can detach from a primary tumor and flow throughout the bloodstream, enabling them to seed distant new tumors. Rare and difficult to capture, only a few circulating tumor cells can be found in a milliliter of blood in a cancer patient. By comparison, the same volume of blood contains several million white blood cells and a billion red blood cells, Hong said.

Three breast cancer cell lines were used as circulating tumor cell models, with each used to compare the cell adhesion of the dendrimer surfaces to a linear polymer of polyethylene glycol. PEG is commonly used to bind molecules to improve the safety and efficiency of therapeutics.

The nano-scale PAMAM dendrimers were chosen because their size and surface dimension could accommodate multiple anti-epithelial cell adhesion molecules, Hong said. This enabled the multivalent binding, along with the physiological process of "cell rolling" induced by E-selectin, which mimics the process by which circulating tumor cells are recruited to the endothelia and enhances the surface sensitivity toward tumor cells.

The surface developed by the UIC research team demonstrated up to a million-fold increase in binding strength, and up to 7-fold increase in detection efficiency, as compared to the aEpCAM-coated PEG surface that is the current gold standard for circulating tumor cell detection.

Hong says this is the first study to capture the tumor cells on the surface exploiting the multivalent effect, which is most likely due to the spherical architecture of dendrimers. The research was selected as a "Hot Paper" by Angewandte Chemie and highlighted in Faculty of 1000 by Donald Tomalia, the inventor of PAMAM dendrimers.

The results demonstrate that the combination of nanotechnology and biomimicry has a "great potential to be applied for highly sensitive detection of rare tumor cells from blood," Hong said.


'/>"/>

Contact: Sam Hostettler
samhos@uic.edu
312-355-2522
University of Illinois at Chicago
Source:Eurekalert

Related medicine news :

1. Flexible rack systems sort molecules
2. Magnetic attraction: NIST/CU microchip demonstrates concept of MRAM for biomolecules
3. A new set of building blocks for simple synthesis of complex molecules
4. Small molecules inhibit growth of human tumor cells
5. Molecules identified that help propel cancer metastasis
6. Small molecules may prevent ebola infection
7. Molecules made by IUPUI students may have potential to cure diseases
8. Link between signaling molecules could point way to therapies for epilepsy, stroke, other diseases
9. New microscopy tracks molecules in live tissue at video rate
10. Fit People Release More Fat-Burning Molecules During Exercise
11. Exploring the Davids and Goliaths of therapeutic molecules
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/26/2017)... , ... April 26, 2017 , ... ... to improve the profitability of ready-to-drink, high-protein beverages by helping beverage manufacturers more ... to dairy proteins, providing direct cost savings as well as more stable pricing ...
(Date:4/25/2017)... ... April 25, 2017 , ... Splashtop ... Mirroring360 Pro . This new addition to the Mirroring360 product family combines device ... business. , Mirroring360 Pro enables educators, business professionals and individuals to stream or ...
(Date:4/25/2017)... ... 25, 2017 , ... As President Trump challenges the status ... his administration could impact the employee benefits industry. James Slotnick, AVP, Government Relations, ... to make it through Congress. His discussion will focus on the current state ...
(Date:4/25/2017)... ... April 25, 2017 , ... Patients who would like ... in a fraction of the time as traditional braces – Wilckodontics®. Dr. Victoria ... now offers this revolutionary treatment with or without a referral. , Wilckodontics ...
(Date:4/25/2017)... ... 25, 2017 , ... Buyers and sellers in the thriving multi-billion dollar cannabis ... –can’t help but be heartened by the industry’s current surge. But another thing that ... “skunk smell.” At last they can simply, safely and effectively end their aroma ...
Breaking Medicine News(10 mins):
(Date:4/20/2017)... April 20, 2017  AbbVie (NYSE: ABBV), a ... (n=145/146) of chronic hepatitis C virus (HCV) infected ... 6 and compensated cirrhosis (Child-Pugh A) achieved sustained ... ) with its investigational, pan-genotypic regimen of glecaprevir/pibrentasvir ... seen following 12 weeks of G/P treatment without ...
(Date:4/19/2017)... 19, 2017 /PRNewswire/ - CRH Medical Corporation (TSX: CRH) (NYSE MKT: CRHM) (the ... Co. Healthcare Investor Conference 2017 at the Sheraton Hotel in ... Chief Executive Officer of the Company is scheduled to present on ... Bear and the Chairman of the Board, Tony Holler ... ...
(Date:4/19/2017)... 19, 2017 Global Prostate Cancer Therapeutics ... the prostate cancer therapeutics market analyzes the current ... prevalence of prostate cancer, launch of promising emerging ... of new drugs & therapeutic biological products, and ... to lesser side effects are some of the ...
Breaking Medicine Technology: