Navigation Links
Molecule regulates production of antibacterial agent used by immune cells
Date:6/20/2014

Researchers have discovered how a protein molecule in immune cells promotes the production of nitric oxide, a potent weapon in the cells' arsenal to defend the body from bacterial attack. The protein may offer a target for reining in the inflammatory response, which must be able to fight infection without damaging tissue.

The study was published in the Journal of Innate Immunity. NFATc3 is one of several related protein molecules known to play a role in regulating genes in the T and B cells of the immune system. Ravi Ranjan, research scientist at the University of Illinois at Chicago College of Medicine, who is first author on the paper, said he and his collaborators wanted to know if NFATc3 also had any function in macrophages -- specialized killer cells that hunt down, engulf and destroy marauding bacteria.

Macrophages kill using chemicals, including nitric oxide, that they synthesize in response to infection. Macrophages are also important in reducing the inflammation in sepsis, an out-of-control reaction to infection that can cause organ failure and death.

When the researchers exposed macrophages to chemicals that signal a bacterial infection, they found that NFATc3 increasingly bound to genes that boost the production of nitric oxide synthase -- the enzyme that makes nitric oxide. The binding of NFATc3 suggests the molecule is turning on those genes and upping the production of nitric oxide. Macrophages deficient in NFATc3 produced much less nitric oxide synthase under the same conditions.

"Without the ability to synthesize inducible nitric oxide synthase, a macrophage would be missing a key element of its chemical weaponry," Ranjan said. "We would expect these cells to be much less effective at killing bacteria and attenuating sepsis."

To test this hypothesis, the researchers then induced sepsis in mice that lacked the ability to make NFATc3. As expected, lung tissue from these mice had a much higher bacterial load than the lung tissue of septic mice that could produce NFATc3.

"Our study demonstrates that NFATc3 is required for macrophages to effectively fight infection, because without it, they can't make their primary bactericidal agent -- nitric oxide," Ranjan said. The immune system must strike a balance between fighting infection and going overboard as it does in sepsis and actually causing harm, Ranjan said.

"An overproduction of nitric oxide can actually contribute to lung injury even as it helps clear bacterial infections," he said.

"An NFATc3 inhibitor, given as a drug to people in septic shock, may be a way to attenuate the harmful effects that come with an overproduction of nitric oxide." Other authors on the paper are Dr. Gye Young Park, assistant professor of clinical medicine, and Dr. Lei Xiao, assistant professor of medicine, both in the division of pulmonary, critical care, sleep and allergy at UIC; Manjula Karpurapu, Jing Deng, Sangwoon Chung, Yong Gyu Lee and Dr. John William Christman of the Ohio State University Wexner Medical Center; and Myungsoo Joo of Pusan University, Korea.


'/>"/>

Contact: Sharon Parmet
sparmet@uic.edu
312-413-2695
University of Illinois at Chicago
Source:Eurekalert

Related medicine news :

1. Mayo Clinic moves small-molecule drugs through blood-brain barrier
2. Molecule acts as umpire to make tough life-or-death calls
3. Molecule linked to aggressive pancreatic cancer offers potential clinical advances
4. Molecules involved in rheumatoid arthritis angiogenesis identified
5. Protein molecule may improve survival in deadly lung disease
6. Faster dental treatment with new photoactive molecule
7. Discovery of anti-appetite molecule released by fiber could help tackle obesity
8. Alcohol-breakdown molecule may play a role in breast cancer development
9. Designer swiss-army-knife molecule captures RNA in single cells in their natural tissue environment
10. Molecule discovered that protects the brain from cannabis intoxication
11. Molecule common in some cancers, rheumatoid arthritis leads to potential therapy for both
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/12/2016)... ... ... The law firm of Morrow, Morrow, Ryan & Bassett is offering scholarships ... is to encourage applicants to pursue a degree in their field of study and ... , “We have available jobs in St. Landry and Evangeline Parishes that can be ...
(Date:2/12/2016)... ... ... Each year, the American Physical Therapy Association (APTA) offers a Combined Sections Meeting. ... Almost 10,000 physical therapists across the country are expected to attend this annual convention ... field and network with their colleagues. As in years past, HydroWorx is proud ...
(Date:2/12/2016)... ... February 12, 2016 , ... Itopia, a leader in cloud ... Clarity Intelligence Platform (CIP) into Cielo®, a discovery, migration and cloud orchestration engine. ... their small and medium business (SMB) clients. , In recent years, ...
(Date:2/12/2016)... ... ... Vail knee specialist Robert LaPrade, MD, PhD was named one ... consists of physicians establishing, leading and partnering with ambulatory surgery centers across the United ... Center, also known as an ASC, is a modern health care facility focused on ...
(Date:2/12/2016)... ... ... For Coast Dental dentist Everet Lake, DDS, the smiles began at dawn. He ... 7 a.m. to volunteer at Friday’s Dentistry from the Heart event in New Port ... hundreds of uninsured and underinsured people receive much-needed dental care. , This was ...
Breaking Medicine News(10 mins):
(Date:2/11/2016)... Feb. 11, 2016  NOIT™ Research LLC, a private, ... of Change" campaign to assist needy families in obtaining ... unit sold between February 10, 2016 and March 31, ... needy family. The NOIT is an auditory stimulus that ... develop language skills. Beth Shier , ...
(Date:2/11/2016)... , Feb. 11, 2016 AAIPharma ... provider of custom manufacturing and development services for ... sterile fill-finish capabilities and capacity in its ... in demand has driven several recent investments. ... it had one filling line with small-scale lyophilization. ...
(Date:2/11/2016)... PORTLAND, Ore. , Feb. 11, 2016 ... to announce the acquisition of SolutionsRx, a full-service 340B ... hospitals. Along with providing traditional contract pharmacy services, SolutionsRx ... assist clients in navigating the complex 340B regulatory environment. ... Wellpartner. --> James R. Love , CEO ...
Breaking Medicine Technology: