The scientists also present results comparing RatCAP-wearing rats moving freely about their cages with animals that had been anesthetized, as well as comparisons of two methods of administering the tracer - injecting it all at once and in a steady infusion to maintain a constant concentration in the blood.
"These measurements will help us further refine the technique and aid in our assessment of results obtained with RatCAP in comparison with other study techniques," Schulz said.
The researchers' next step will be to use RatCAP to explore distinct behavioral expressions that can be correlated with simultaneously acquired PET data.
The research was funded by the DOE Office of Science. Development of RatCAP was a joint effort between Brookhaven's Medical, Chemistry and Physics Departments, Instrumentation Division and the Biomedical Engineering Department at Stony Brook University. Co-authors on the paper include: Daniela Schulz (Brookhaven), Sudeepti Southekal (Brigham & Women's Hospital, Boston),Sachin S. Junnarkar (Brookhaven), Jean-Franois Pratte (Universit de Sherbrooke, Sherbrooke, Canada), Martin L. Purschke (Brookhaven), Paul O'Connor (Brookhaven), Sean P. Stoll (Brookhaven), Bosky Ravindranath (Stony Brook), Sri Harsha Maramraju (Stony Brook), Srilalan Krishnamoorthy (Stony Brook), Fritz A. Henn (Brookhaven and Cold Spring Harbor Laboratory), Craig L. Woody (Brookhaven), and David J. Schlyer and Paul Vaska (Brookhaven and Stony Brook).
All research involving laboratory animals at Brookhaven National Laboratory is conducted under the jurisdiction of the Lab's Institutional Animal Care and Use Committee (IACUC, http://www.bnl.gov/ora/IACUC.asp) in compliance with the Public Heath Service (PHS) Policy on Humane Care and Use of Laboratory Animals (http://grants.nih.gov/grants/olaw/references/phspol.htm), the U.S. Department of Agriculture's Animal Welfare A
|Contact: Karen McNulty Walsh|
DOE/Brookhaven National Laboratory