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Microgrid allows simultaneous study of multiple variables
Date:10/10/2007

UPTON, NY -- Scientists at the U.S. Department of Energy's Brookhaven National Laboratory have developed a method for correlating the results of microscopic imaging techniques in a way that could lead to improved understanding, diagnosis, and possibly treatment of a variety of disease conditions, including Alzheimer's disease. The Laboratory has filed a U.S. provisional patent application for the invention.

The invention is essentially a micron-scale metallic marking grid upon which scientists place their samples -- biological tissues or inorganic samples such as minerals -- prior to imaging with different methods. "When the findings are analyzed, the grid can be used to 'map,' or orient, the images to one another, allowing us to study multiple variables in a single sample and better understand how they relate to one another," said biophysicist Lisa Miller, leader of the team that developed the new method.

For example, many diseases such as Alzheimer's are characterized by changes in both organic materials, such as proteins, as well as changes in the composition or concentration of inorganic trace metals (e.g., iron, copper, and zinc). Scientists have techniques -- infrared spectroscopy and x-ray fluorescence -- for studying each of these independently. But without a way to correlate the findings from the two methods, important information about the relationship between the organic and inorganic components can be missed.

"The x-ray and infrared-sensitive grid allows for the study of both pathological symptoms by precisely overlapping the results of these imaging methods," Miller said. "This ability to correlate images will ultimately lead to a more complete picture of many disease states."

The grid is deposited in two thicknesses onto an x-ray transparent material like mylar. It can be made of any metal, but gold is preferred. The "bars" of the grid are only a couple of nanometers thick, whereas the remainder of the metal sur
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Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert

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