Navigation Links
Mice with human body's defenses

Therapeutic antibodies can be an efficient alternative when common drugs do not work anymore. However, antibodies obtained from blood of animals such as mice could not be used: The human immune system recognizes them as foreign and rejects them. In an international cooperation, scientists from the Helmholtz Centre for Infection Research (HZI) in Braunschweig, Germany have now succeeded in developing a promising approach to solve this problem; with the help of human stem cells they generated mice with a human immune system, which were then vaccinated to produce human monoclonal antibodies. These fully human antibodies could help in the research and therapy of human diseases. Their results have now been published in the current online issue of the scientific journal "PLoS One".

Antibodies are small proteins, produced by B cells during an immune response. They bind at and thus mark invading pathogens so that scavenger cells recognize and destroy them. "The task of our immune system is to distinguish between self and non-self structures," says Professor Carlos A. Guzmn, head of the department of "Vaccinology and Applied Microbiology" at the HZI. "This means also that only human antibodies come into question for an antibody therapy", since the human immune system fights antibodies from mice a threat for the patient. Furthermore, it is cumbersome to humanize murine antibodies for human treatment or to generate human B cell clones producing high quantities of antibodies.

The scientists used an already established method to give a human immune system to mice, which were then exploited to solve this problem: they injected human stem cells into young mice that due to a genetic defect lack an immune system. The stem cells migrate into the bone marrow, proliferate, differentiate and lead to the generation of a human immune system. "In our in-depth investigations we were able to detect all important types of immune cells in these mice," says Dr. Pablo Becker, scientist in the HZI department "Vaccinology and Applied Microbiology".

To validate the new approach, mice with a human immune system were vaccinated against Hepatitis B or Tetanus. The scientists then isolated human antibody producing B cells from the mice and treated them so that they survive outside the body in a cell culture dish and continue producing antibodies. Then, the researchers took a deeper look at the antibodies. The results give hope: "Antibodies from mice with a human immune system showed good properties in our tests, but the model still needs to be improved for broad implementation in biomedicine," says Pablo Becker. "However, we were able to demonstrate for the first time that it is possible to produce human monoclonal antibodies using humanized mice." Now it is important to improve this mouse model to use it one day for the development of advanced therapies against human diseases. "In the future this approach might represent the most powerful tool to develop therapeutic antibodies for clinical use," hopes Becker.


Contact: Dr. Bastian Dornbach
Helmholtz Association of German Research Centres

Related medicine news :

1. NIH scientists describe how salmonella bacteria spread in humans
2. New research technology to target human gut bacteria
3. Welsh scientists clone human virus
4. Scientists find link in humans between nerve cell production, memory
5. Human neural stem cells restore motor function in mice with chronic spinal cord injury
6. Input-output trade-offs found in human information processing
7. Human Meds Are Pets Biggest Poisoning Danger
8. NIH launches effort to define markers of human immune responses
9. New human adenovirus may not make for good vaccines, after all
10. Human clinical trial of NIH-developed dengue vaccine begins
11. Human embryonic stem cells purified in new, rapid technique
Post Your Comments:
(Date:11/29/2015)... Los Angeles, Ca (PRWEB) , ... November 29, 2015 , ... ... from the American Board of Ophthalmology on November 25th 2015. Peer Certification ... in the field of his specialty. Certification in Ophthalmology is first obtained after the ...
(Date:11/29/2015)... ... November 29, 2015 , ... Effective immediately, every single IguanaMed scrub ... on Black Friday Target is offering a “Buy One Scrub Set, Get the 2nd ... opportunity to purchase IguanaMed at a discounted price. , IguanaMed’s mission is ...
(Date:11/28/2015)... ... November 28, 2015 , ... Trying to relax on a couch ... BUDDY. "I conceived of this design due to personal experience with a bad back," ... promotes relaxation and convenience, as well as increases support. It also makes it easier ...
(Date:11/27/2015)... , ... November 27, 2015 , ... According to an ... recent 2015 American Dental Association meeting in Washington D.C. revolved around the fact that ... patient’s overall health. The talk stressed the link between periodontal disease (more commonly referred ...
(Date:11/27/2015)... ... November 27, 2015 , ... A team of Swiss doctors has released ... Surviving Mesothelioma has just posted the findings on the website. Click here to ... cases of 136 mesothelioma patients who were treated with chemotherapy followed by EPP surgery. ...
Breaking Medicine News(10 mins):
(Date:11/26/2015)... , November 26, 2015 ... of the "2016 Future Horizons and ... Abuse Testing Market: Supplier Shares, Country Segment ... to their offering. --> ... "2016 Future Horizons and Growth Strategies ...
(Date:11/26/2015)... 26, 2015 ... of the  "2016 Future Horizons and ... Drug Monitoring (TDM) Market: Supplier Shares, ... Opportunities"  report to their offering.  ... the addition of the  "2016 Future ...
(Date:11/26/2015)... 26, 2015 ... of the  "2016 Future Horizons and ... Surface Marker Testing Market: Supplier Shares, ... their offering.  --> ... the  "2016 Future Horizons and Growth ...
Breaking Medicine Technology: