Navigation Links
Method of DNA repair linked to higher likelihood of genetic mutation
Date:2/16/2011

INDIANAPOLIS Researchers from Indiana University-Purdue University Indianapolis (U.S.A) and Umea University (Sweden) report in a study published in the February 15, 2011, issue of PLoS Biology that a method by which cells repair breaks in their DNA, known as Break-induced Replication (BIR), is up to 2,800 times more likely to cause genetic mutation than normal cell repair.

Accurate transmission of genetic information requires the precise replication of DNA. Errors in DNA replication are common and nature has developed several cellular mechanisms for repairing these mistakes. Mutations, which can be deleterious (development of cancerous cells), or beneficial (evolutionary adaption), arise from uncorrected errors. When one or many cells repair themselves using the efficient BIR method, accuracy is lost.

"When BIR occurs, instead of using a "band aid" to repair a chromosomal break, the broken piece invades another chromosome and initiates replication which happens at the wrong place and at the wrong time and probably with participation of wrong proteins," said Anna Malkova, Ph.D., associate professor of biology at the School of Science at IUPUI, who led the study.

The researchers used yeast to investigate the level of mutagenesis associated with BIR and found that the method's proclivity to cause mutation was not affected by where on the DNA the repair was made.

Why is BIR so inaccurate as compared to normal replication?

"We didn't find a smoking gun," said Malkova, also an adjunct associate professor of medical and molecular genetics at the Indiana University School of Medicine. "We think there are at least four changes to replication machinery that must occur to create a perfect storm or synergy which make BIR repair so mutagenic."

For example, during BIR, the researchers found a dramatic increase in concentration of nucleotides -- the building blocks used to form DNA.

"Our findings strongly suggest that mutagenesis caused by BIR doesn't happen slowly, it occurs in surges sudden bursts which may lead to cancer," said Malkova, who is a geneticist. "We plan to continue investigating BIR in the hope of finding clues as to why this mechanism of cell repair is so likely to cause mutations. The ultimate goal, of course, is to prevent those mutations that cause cancer."


'/>"/>

Contact: Cindy Fox Aisen
caisen@iupui.edu
317-274-7722
Indiana University-Purdue University Indianapolis School of Science
Source:Eurekalert  

Related medicine news :

1. Study reveals security weaknesses in file-sharing methods used in clinical trials
2. Scientists develop method to identify fleetingly ordered protein structures
3. Scripps Research scientists develop powerful new methodology for stabilizing proteins
4. MRI: An accurate method to evaluate iron overload
5. Patent awarded for method to dampen immune response
6. New method for making large quantities of deuterium-depleted drinking water
7. Faster, scalable method for producing AAV-based gene transfer vectors
8. Better Methods Needed to Measure Hospital Quality: Experts
9. A new method is developed for predicting shade improvement after teeth bleaching
10. New Method Might Raise Number of Donor Lungs
11. Uniform Methods Urged for Grading Hospital Report Cards
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Method of DNA repair linked to higher likelihood of genetic mutation
(Date:8/17/2017)... ... August 17, 2017 , ... Dr. Charles W. Grimsley’s ... ($15.99, paperback, 9781498497626; $7.99, eBook, 9781498497633) focuses on the treatment of veterans ... and achieve forgiveness, through a progressive journey toward healing. This book will help ...
(Date:8/17/2017)... ... August 17, 2017 , ... Inc. Magazine Unveils 36th ... No. 132 with Three-Year Sales Growth of 3,004.8% , NEW YORK, August 16, 2017 ... 5000, the most prestigious ranking of the nation's fastest-growing private companies. The list represents ...
(Date:8/17/2017)... ... August 17, 2017 , ... Momkus McCluskey Roberts LLC recently ... is a member of the firm’s Commercial Litigation and Employment Law groups. , Ms. ... of knowledge in matters of employment litigation, commercial litigation and business disputes. Her experience ...
(Date:8/17/2017)... ... August 17, 2017 , ... Centrifugation is more than just spinning. ... that we can no longer do without. And just like a car, there are ... , In this webinar, attendees will learn about the most important safety aspects while ...
(Date:8/17/2017)... ... August 17, 2017 , ... A recent report indicates that ... industry in 2016 cited deficiencies in data integrity. The FDA outlines their expectations for ... , Presented as part of the Beckman Coulter Life Sciences Virtual Trade ...
Breaking Medicine News(10 mins):
(Date:8/2/2017)... and BENTON, Ky. , Aug. 2, 2017   Marshall County ... its environmental services (ES) team to proactively reduce the risk of hospital-acquired infections (HAIs). The ... disinfection robot that uses UVC light energy to kill deadly pathogens such as C. ... Tru-D SmartUVC ... Tru-D in action in ...
(Date:8/2/2017)... 2017 CaryRx, a next-generation full-service pharmacy, has announced ... patients in the Washington D.C. metropolitan ... providing delivery of medications through the convenience of its patient-friendly ... delivered within one hour to any location in D.C. ... this invaluable service to Washington D.C. ," says ...
(Date:7/31/2017)... , July 31, 2017  Diplomat Pharmacy, Inc. (NYSE: DPLO), ... Michigan by Crain,s Detroit Business ... and public companies by three-year revenue growth. This year,s edition ... To view the complete list, visit crainsdetroit.com/awards/fast_50/2017 ... is an incredible triumph," said Phil Hagerman , CEO ...
Breaking Medicine Technology: