Navigation Links
Mayo researchers provide atomic view of a histone chaperone
Date:3/1/2012

ROCHESTER, Minn. -- Mayo Clinic researchers have gained insights into the function of a member of a family of specialized proteins called histone chaperones. Using nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography, they have determined the 3-D structure and interactions of the histone chaperone Rtt106 down to the atomic details. The findings are published in the journal Nature.

"The interactions we described are important for gene silencing and the DNA damage response," says senior author Georges Mer, Ph.D., a Mayo Clinic structural biologist. "This is exciting because our newfound knowledge will help us better understand these fundamental cellular processes."

In cells, our DNA is part of a structure called chromatin, comprised of proteins, the majority called histones, which are wrapped with the DNA. Associated with the histones is another group of proteins called histone chaperones, which promote the proper assembly or disassembly of the chromatin during the times our DNA is replicated or repaired when damaged. Their dysfunction has been linked to cancer, aging and other diseases.

The Discovery

Before this Mayo study, scientists knew that the histone chaperone Rtt106 helped in the deposition of histones -- specifically, a complex of histones H3 and H4 -- onto the replicating DNA. They did not understand how Rtt106 does this, given that it does not possess any of the known requirements. Histone H3 is in a modified form where one of its amino acids, lysine 56, is acetylated. Rtt106 does not seem to have an acetylated lysine reader domain.

Mayo researchers discovered two novel domains in Rtt106 that take on this role. One, termed the homodimerization domain, allows two molecules of Rtt106 to be linked so they can cooperate in binding histones H3 and H4. The other, called the double PH domain, is responsible for sensing the acetylated lysine of H3 and further reinforces the interaction. The combined actions of the two domains of Rtt106 enable it to perform the chaperoning function efficiently and properly. This is the first time anyone has described this mode of specific association between a histone chaperone and a modified histone complex.


'/>"/>

Contact: Robert Nellis
newsbureau@mayo.edu
507-284-5005
Mayo Clinic
Source:Eurekalert

Related medicine news :

1. Researchers find potential solution to melanomas resistance to vemurafenib
2. Kessler Foundation researchers present at DC Conference on Race, Ethnicity and Disabilities
3. Researchers Spot Genes Behind Macular Degeneration
4. 90 percent of firefighters exhibit symptoms of PTSD according to Ben-Gurion University researchers
5. New blood test for early cancer detection developed by Ben-Gurion University researchers
6. UC Davis MIND Institute researchers to present on autism at AAAS Annual Meeting in Vancouver
7. Nasty superbug is being studied by UB researchers
8. Researchers see differences in Autism brain development as early as 6 months
9. As diabetes emerges, researchers track diseases first steps
10. Salk researchers find new drug target for lung cancer
11. GW researchers reveal digital transcriptome of breast cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... (PRWEB) , ... October 13, 2017 , ... ... established the certification process to promote standards of excellence for the field of ... scheduled for March 22 – 25, 2018 in Orlando, Florida at the Omni ...
(Date:10/13/2017)... , ... October 13, 2017 , ... Many families have ... many long-term care insurance companies have a waiver for care if the client has ... period, when the family pays for care, is often waived, so the benefits from ...
(Date:10/13/2017)... ... October 13, 2017 , ... Talented host, actor Rob ... sciatica in a new episode of "Success Files," which is an award-winning educational ... and investigates each subject in-depth with passion and integrity. , Sciatica occurs when ...
(Date:10/12/2017)... ... October 12, 2017 , ... CitiDent ... sleep apnea using cutting-edge Oventus O2Vent technology. As many as 18 million ... by frequent cessation in breathing. Oral appliances can offer significant relief to about ...
(Date:10/12/2017)... , ... October 12, 2017 , ... ... a leader in post-acute health care, have expanded their existing home health joint ... Health. , AccentCare has been operating a joint venture home health company with ...
Breaking Medicine News(10 mins):
(Date:10/10/2017)... -- NDS received FDA 510(k) clearance in May 2017 for its highly ... designed for endoscopy environments. An innovative secondary monitor solution, ZeroWire ... support the improvement of patient outcomes, procedural efficiency, and the lowering ... ... ...
(Date:10/4/2017)... 2017  According to the Centers for Disease Control and Prevention (CDC), ... PhysicianOne Urgent Care is helping communities across Massachusetts , ... by offering no-cost* flu shots through the end of the month. ... insurance regulations. ... get a flu shot is by the end of October, according to ...
(Date:10/2/2017)... Halo Labs announces the European launch of their new ... at MIBio 2017 in Cambridge, U.K on ... in biopharmaceutical samples with unprecedented speed and sensitivity while using far ... Membrane Imaging. ... particle analysis system ...
Breaking Medicine Technology: