Navigation Links
Major Advance in the Observation of DNA Molecules
Date:12/7/2007

EVRY, France, December 7 /PRNewswire/ -- The teams of Eric Le Cam, from the "Molecular Interactions and Cancer" lab (UMR 8126 - Institut Gustave Roussy/CNRS/Universite Paris Sud) and David Pastre, from the "Structure and Activity of Normal and Pathological Biomolecules - SABNP" lab (Inserm/Universite d'Evry-Val-d'Essonne, U829, Genopole Evry) have successfully developed a new method for observing DNA using atomic force microscopy (AFM) under intracellular physico-chemical conditions. These findings, published in the Journal "Biomacromolecules" on November 19, 2007, were validated by the observation of various degrees of DNA compaction -- a key factor in replication and transcription. This method will facilitate research into DNA/protein interactions and their impacts on DNA activity, DNA chips and nanobiotechnology.

The first phase of these investigations was to find a method for absorbing DNA on a mica surface (a clay mineral traditionally used in biomolecular imaging), in the presence of monovalent salts. These findings, themselves representing a major advance in current techniques, make it possible to observe DNA molecules using atomic force microscopy (AFM) under intracellular physico-chemical conditions (molecular crowding, monovalent salts). Until recently, it was in fact impossible to obtain nanometric 3D high resolution images of DNA molecules using AFM under such lifelike conditions.

To validate this method and demonstrate the relevance of these novel imaging conditions, researchers chose to examine how macromolecular crowding affected the compaction of DNA molecules. The presence of very large molecules in the intracellular environment impacts directly the degree of DNA compaction, and thus its activity. This phenomenon, which is well-known among biologists, but poorly studied has a critical influence on replication, transcription and, consequently, on gene expression.

This method enables the observation of DNA molecules in various physiological conditions, in particular in interaction with proteins; it can also be used in the fields of DNA chips or nanobiotechnology.

Reference: "Atomic Force Microscopy Imaging of DNA under Macromolecular Crowding Conditions"

David Pastre, Loic Hamon, Alain Mechulam, Isabelle Sorel, Sonia Baconnais, Patrick Curmi, Eric Le Cam, Olivier Pietrement, Biomacromolecules, 2007, Nov 19.

Genopole(R)

Genopole(R) is France's first biopark dedicated to research into genetics and biotechnology. The campus is home to private and public research laboratories, biotech companies as well as university teaching facilities (University of Evry Val d'Essonne). With 21 research labs on campus and a portfolio of 62 biotech companies, therapeutic innovation is the core concern of Genopole(R) stakeholders. Its objective is to promote the development of research into genomics, post-genomics and related sciences and transfer technology to the industrial sector, develop advanced learning in these areas, and create and support biotech companies. http://www.genopole.fr


'/>"/>
SOURCE Genopole
Copyright©2007 PR Newswire.
All rights reserved

Related medicine news :

1. Majority of 2.4 Million U.S. Children With ADHD Not Diagnosed or Consistently Treated, According to New Gold Standard Study by Cincinnati Childrens Researchers
2. Laboratory at UI plays major role in diagnosing cancer
3. Draxis Health selected by Johnson and Johnson Consumer Companies, Inc. for major contract manufacturing relationship
4. DaVita Acquires Majority Stake in HomeChoice Partners, Inc.
5. Major Pharma Clinical Case Studies From Amgen, AstraZeneca, GSK, Intermune, Cephalon Inc, Sunesis Pharmaceuticals and Dartmouth Medical School During Fall Clinical Focused Programs
6. Major grant advances UWs clinical and translational research enterprise
7. Major differences revealed in how local authorities in the UK support disabled people
8. Watson Wyatt Identifies Major Benefit Trends During Open Enrollment Season
9. Worldwide Probiotic Drink, Yakult(R), Launches in Its First Major U.S. Market
10. Ericom Provides Significant Cost-Savings for Major New York City Healthcare Organization
11. Eckert Seamans Announces Major Expansion in Philadelphia, West Chester and Harrisburg
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/5/2016)... ... February 05, 2016 , ... Stuart Bentkover, MD, FACS ... PicoSure. Designed to provide the most effective tattoo removal today, Dr. Bentkover is the ... , Developed by Cynosure, the PicoSure has been approved by the Food and Drug ...
(Date:2/5/2016)... ... February 05, 2016 , ... In sleep, when the defenses of the day ... feature of patients with eating disorders is significant self-criticism, and consequently these patients experience ... are regarded as maladaptive means for coping with this unease, but also leads to ...
(Date:2/5/2016)... ... February 05, 2016 , ... On June 9-10, Las Vegas ... medical education (CME) event presented by the Association for Comprehensive Care in Rare ... ACCORD, whose mission is to provide education, tools, and resources to primary care ...
(Date:2/5/2016)... ... February 05, 2016 , ... –This week, Atascadero water heater ... having a tankless water heater. To view the report, click here or ... tankless. While each has their pros and cons, the type chosen is almost entirely ...
(Date:2/4/2016)... ... February 04, 2016 , ... Dr. Carol Francis and ... February 19-21, 2016, in Southern California . The International Hypnosis Federation 2016 Conference ... professional speakers, including Clinical Psychologist and Hypnotherapist Dr. Carol Francis (topics ...
Breaking Medicine News(10 mins):
(Date:2/4/2016)... 4, 2016 Mettler-Toledo International Inc. (NYSE: ... Provided below are the highlights: , Sales ... with the prior year.  Reported sales decreased 3% as ... , Net earnings per diluted share as reported ... year period.  Adjusted EPS was $4.65, an increase of ...
(Date:2/4/2016)... 4, 2016  AMRI (NASDAQ:  AMRI) today announced that ... and President of Pfizer Global Supply, has been elected to ... 2016. In addition, the Company announced that Mr. Gabe ... since 2010, has retired from the AMRI Board of Directors ... other business ventures.  William S. Marth , ...
(Date:2/4/2016)... Feb. 4, 2016 SONIFI™ Health, the ... today announced that MonteCedro, an innovative retirement community, ... System. The system provides a simple and intuitive ... through a tablet PC. ... system provides access to a wide spectrum of ...
Breaking Medicine Technology: