In a development that could lead to faster and more effective toxicity tests for airborne chemicals, scientists from Rice University and the Rice spinoff company Nano3D Biosciences have used magnetic levitation to grow some of the most realistic lung tissue ever produced in a laboratory.
The research is part of an international trend in biomedical engineering to create laboratory techniques for growing tissues that are virtually identical to those found in people's bodies. In the new study, researchers combined four types of cells to replicate tissue from the wall of the bronchiole deep inside the lung.
The research is available online and scheduled to appear in a future issue of the journal Tissue Engineering Part C: Methods.
"One of the unique things about the magnetic levitation technology is that it allows us to move cells around and arrange them the way that we want for a particular types of tissue," said study co-author Tom Killian, professor and department chair of physics and astronomy at Rice. "This is the first time anyone has arranged these four cell types in the same way that they are found in lung tissue."
In vitro laboratory tests have historically been conducted on 2-D cell cultures grown in flat petri dishes, but scientists have become increasingly aware that cells in flat cultures sometimes behave and interact differently than cells that are immersed in 3-D tissue.
Killian and fellow scientists from Rice and the University of Texas MD Anderson Cancer Center co-founded Nano3D Biosciences in 2009 after creating a technology that uses magnetism to levitate and grow 3-D cell cultures. The technology relies on inert, nontoxic magnetic nanoparticles that are inserted into the living cells. Researchers can then use magnets to lift and suspend the cells as they grow and divide.
"Growing realistic lung tissues in vitro is a particular challenge," said study co-author Jane Grande-Allen, profess
|Contact: David Ruth|