Navigation Links
Laser-light testing of breast tumor fiber patterns helps show whose cancer is spreading

Using advanced microscopes equipped with tissue-penetrating laser light, cancer imaging experts at Johns Hopkins have developed a promising, new way to accurately analyze the distinctive patterns of ultra-thin collagen fibers in breast tumor tissue samples and to help tell if the cancer has spread.

The Johns Hopkins researchers say their crisscrossing optical images, made by shining a laser back and forth across a biopsied tissue sample a few millionths of a meter thick, can potentially be used with other tests to more accurately determine the need for lymph node biopsy and removal in women at risk of metastatic breast cancer.

In what is believed to be the first study to measure minute changes in tumor connective tissue fibers, researchers found that eight women whose cancers had spread beyond the breast through the body's lymphatic system had about 10 percent more densely packed and radially spread-out collagenous structural proteins than six women whose cancers had not yet spread. Collagen fibers in the non-metastasized tumors, also obtained during breast biopsy, were more diffuse and arranged in a transverse or horizontal pattern. All 14 women in the study had aggressive, malignant breast cancer.

In the new report, to be published in the Journal of Biomedical Optics online Nov. 1, researchers say that if these "proof of principle" findings hold up in testing now under way in hundreds more women with or without metastatic breast cancer, then their new optical imaging tool could simplify testing for spreading disease and help people avoid unnecessary lymph node surgery.

"Our new diagnostic technique has the potential to help reassure thousands of breast cancer patients that their cancers have not spread to other organs, and could help them avoid the risks and pain currently involved in direct inspections of lymph nodes for the presence of cancerous cells," says study senior investigator Kristine Glunde, Ph.D.

Women with denser tumor fiber patterns would likely stand a greater chance of needing lymph node biopsy and removal and inspection of such tissue for malignant cells, says Glunde, an associate professor at the Johns Hopkins University School of Medicine Russell H. Morgan Department of Radiology and the Sidney Kimmel Comprehensive Cancer Center.

Glunde says complications from lymph node biopsy and more invasive dissection include risk of infection, pain, severe swelling and leakage of lymph fluid around the armpit, as well as stiffening in the arm, which can be permanent. An estimated 230,000 Americans were diagnosed in 2011 with invasive breast cancer, while another 57,000 were found to have noninvasive, or in-situ breast cancer.

Cancer imaging experts have known for more than a decade that the fibrous connective tissue located between cancer cells changes and bunches together as tumors grow and disease spreads, says study co-investigator Zaver Bhujwalla, Ph.D., a professor at Johns Hopkins and its Kimmel Cancer Center.

"Until now, however, we had no proof in principle that such minute and progressive changes outside cancer cells, in the tumor micro-environment or extracellular matrix, could be measured and potentially used to better guide our staging and treatment decisions," says Bhujwalla, who also serves as director of the Johns Hopkins In Vivo Cellular and Molecular Imaging Center (ICMIC), where the latest imaging study was performed.

It was also at ICMIC in 2010, supported with funds from the U.S. National Cancer Institute (NCI), that Glunde, Bhujwalla and fellow study co-investigator Meiyappan Solaiyappan, B.S., developed the specialized computer software used to analyze the microscopic spaces between tumor collagen fibers and calculate their density.

The tissue fiber images were obtained using an optical imaging technique called second harmonic generation microscopy, in which a long-wavelength laser light is deflected off the collagen fibers for a few seconds, allowing for several planes and fields of view to be captured. The longer infrared wavelength, at 880 micrometers, was chosen because it can penetrate the tissue beyond the colorful light waves visible to the human eye, but does not damage and heat up the cancer cells, as a slightly longer infrared wavelength would. Glunde says the many fields of view were randomly taken throughout the tissue sample, providing a "realistic representation of each breast cancer sample." Breast biopsy samples came from tissue research collections in Maryland.


Contact: David March
Johns Hopkins Medicine

Related medicine news :

1. Water quality study shows need for testing at state migrant camps
2. Researchers show cost-effectiveness of HIV testing in drug abuse treatment programs
3. Engineers improve blood testing technology with smartphone app, hand-held biosensor
4. Joint EACPR and AHA statement empowers health care professional to use Clinical Cardiopulmonary Exercise Testing
5. PSA Testing Linked to Improved Prostate Cancer Survival
6. Sensor detects glucose in saliva and tears for diabetes testing
7. Prostate cancer survival rates improved since introduction of PSA testing
8. HIV home testing kits prove their worth
9. By studying animal health, researchers find improved ways for developing, testing cancer therapies
10. Heterogeneous ER+ breast cancer models allow more accurate drug testing
11. What would happen without PSA testing?
Post Your Comments:
(Date:11/26/2015)... , ... November 26, 2015 , ... ... abuse located in central Michigan, have come together on Thanksgiving Day to share ... video, available for viewing on the Serenity Point YouTube channel, patients displayed what ...
(Date:11/26/2015)... ... November 26, 2015 , ... Inevitably when people think Thanksgiving, they ... to buy during the Black Friday and Cyber Monday massage chair sales ... the Internet high and low to find the best massage chair deals, they can ...
(Date:11/26/2015)... ... November 26, 2015 , ... ... eReferral system for diagnostic imaging in the Waterloo region. Using the Ocean Platform, ... Medicine tests directly from their electronic medical record (EMR) without the need for ...
(Date:11/26/2015)... Ashburn, VA (PRWEB) , ... November 26, 2015 ... ... Planners, an American Express Travel Representative. As a franchise owner, Somu now offers ... range of cruise, destination wedding packages, private cruise sales, as well as, cabin ...
(Date:11/26/2015)... ... November 26, 2015 , ... The Catalent ... and the need to integrate dose form selection in early phase drug development. ... membership organization supporting and bringing together the UK’s emerging life sciences companies, corporate ...
Breaking Medicine News(10 mins):
(Date:11/26/2015)... PUNE, India , November ... --> ... / personal emergency response system ... grow steadily for 5 years ... growing region expected to see ...
(Date:11/26/2015)... Bajos, November 26, 2015 Un ... de Bremachlorin para el cáncer avanzado.   ... la terapia fotodinámica de Bremachlorin para el cáncer avanzado. ... combina la inmunoterapia con la terapia fotodinámica de Bremachlorin ... Cancer Research . --> Clinical Cancer Research ...
(Date:11/26/2015)... 26 november 2015 AAIPharma Services ... geplande investering aan van ten minste $15,8 ... en het mondiale hoofdkantoor in ... resulteren in extra kantoorruimte en extra capaciteit ... groeiende behoeften van de farmaceutische en biotechnologische ...
Breaking Medicine Technology: