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Laser blasts viruses in blood
Date:9/4/2007

A father-son research team working from separate laboratory benches across the country has discovered a new use for lasers - zapping viruses out of blood. The technique, which holds promise for disinfecting blood for transfusions, uses a low-power laser beam with a pulse lasting just fractions of a second.

Johns Hopkins University student Shaw-Wei David Tsen says it was during a stroll in the park with his father that the idea was born. Tsen, an immunology researcher in the laboratory of T.C. Wu at Hopkins Kimmel Cancer Center, sought a new method to rid isolated blood of dangerous pathogens, including the viruses HIV and hepatitis C. He says current techniques using UV irradiation and radioisotopes can leave a trail of mutated or damaged blood components.

Using ultrasonic vibrations to destroy viruses was one possibility, but his father, Kong-Thon Tsen, a laser expert at Arizona State University, had a better idea: Lasers, unlike ultrasound, can penetrate energy-absorbing water surrounding the viruses and directly vibrate the pathogen itself.

The researchers aimed a low-power laser with a pulse lasting 100 femtoseconds (10-13 second) into glass tubes containing saline-diluted viruses that infect bacteria, also known as bacteriophages. The amount of infectious virus within each cube plummeted 100- to 1000-fold after the laser treatment. I had to repeat the experiment several times to convince myself that the laser worked this well, says the younger Tsen.

His laser is different from those emitting a continuous beam of visible light. Our laser repeatedly sends a rapid pulse of light and then relaxes, allowing the solution surrounding the virus to cool off, Tsen says. This significantly reduces heat damage to normal blood components.

Building on the idea that vibration wrecks a virus outer shell, the scientists found that their low-power laser selectively destroys viruses and spares normal human cells around them
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Contact: Valerie Mehl
mehlva@jhmi.edu
410-955-1287
Johns Hopkins Medical Institutions
Source:Eurekalert

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