Navigation Links
Lab-made tissue picks up the slack of Petri dishes in cancer research
Date:1/11/2012

BOSTON (January 11, 2012) New research demonstrates that previous models used to examine cancer may not be complex enough to accurately mimic the true cancer environment. Using oral cancer cells in a three-dimensional model of lab-made tissue that mimics the lining of the oral cavity, the researchers found that the tissue surrounding cancer cells can epigenetically mediate, or temporarily trigger, the expression or suppression of a cell adhesion protein associated with the progression of cancer. These new findings support the notion that drugs that are currently being tested to treat many cancers need to be screened using more complex tissue-like systems, rather than by using conventional petri dish cultures that do not fully manifest features of many cancers.

"Research on cancer progression has been drawn largely using models that grow cancer cells in plastic dishes. Our research reveals a major shortcoming in the experimental systems used to study cancer development. When using simplified culture systems in which cells are grown on plastic, cancer cells grow as a two dimensional monolayer and lack the three-dimensional tissue structure seen in human cancer. As a result, complex interactions that occur between the cancer cells and the surrounding tissue layers are not accounted for," said first author Teresa DesRochers, PhD, a graduate of the Sackler School of Graduate Biomedical Sciences at Tufts, currently in the department of biomedical engineering at Tufts University School of Engineering.

The researchers report that the three-dimensional network of cell interactions activates epigenetic mechanisms that control whether genes critical for cancer development will be turned on or off. By imitating the structure of the tumor microenvironment seen in different stages of cancer, the research team was able to observe that cell-to-cell interactions that are inherent in tissue structure are sufficient to turn on the cell adhesion protein, E-cadherin, that can delay cancer development.

Since both invasion and metastasis occur when cells break away from the primary cancer site, an event correlated with loss of E-cadherin, treating cancers to induce re-expression of this protein through epigenetic control may be an important way to control cancer progression.

"Our findings show the reversible nature of E-cadherin when cancer cells are placed in a three-dimensional network of cells that mimics the way cancer develops in our tissues. This confirms that cancer biology needs to move into the "third dimension" where cancer cells can be studied in a network of other cells that can control their behavior. We know now that the plastic dish alone is not good enough," said senior author Jonathan Garlick, DDS, PhD, a professor in the oral and maxillofacial pathology department at Tufts University School of Dental Medicine.

Jonathan Garlick is also a member of the Cell, Molecular & Developmental Biology program faculty at the Sackler School at Tufts and the director of the Center for Integrated Tissue Engineering (CITE) at Tufts University School of Dental Medicine.

This study, published in the January issue of Epigenetics, was performed in collaboration with Laurie Jackson-Grusby, PhD, associate in pathology at Children's Hospital, Boston, and assistant professor at Harvard Medical School. Additional authors of the study are Yulia Shamis, MSc, a PhD student at the Sackler School of Graduate Biomedical Sciences; Addy Alt-Holland, MSc, PhD, an assistant professor at Tufts University School of Dental Medicine; Yasusei Kudo, DDS, PhD, and Takashi Takata, DDS, PhD, both of the department of oral and maxillofacial pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Japan; and Guangwen Wang, PhD, previously a fellow at Children's Hospital Boston, now a senior scientist at Stemgent.


'/>"/>

Contact: Siobhan E. Gallagher
617-636-6586
Tufts University, Health Sciences Campus
Source:Eurekalert

Related medicine news :

1. Lab-made skin cells will aid transplantation, cancer, drug discovery research
2. New study suggests stem cells sabotage their own DNA to produce new tissues
3. Scientists Discover Molecular Pathway for Organ Tissue Regeneration and Repair
4. Step Up to Speak Up Events Use Spoken Word and African-American Greek Tradition of Stepping to Raise Awareness for Organ and Tissue Donation
5. CSMG Technologies, Inc. Engages New Team to Manage Live Tissue Connect, Inc.
6. Paired drugs kill precancerous colon polyps, spare normal tissue
7. Tissue engineers create a new way to assemble artificial tissues
8. Ultrasound could boost tissue implant success
9. Color-coded tracking method helps scientists analyze outcomes of newly transplanted tissue
10. Molecular Signatures in Post-Mortem Brain Tissue of Younger Individuals at High Risk for Alzheimer's Disease
11. Scholar calls for a new legal, ethical framework for research with human tissue specimens
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/19/2017)... ... September 19, 2017 , ... ... a neurosurgeon, has received a big bump in sales after recently going ... currently available on the Wal-Mart Stores-owned Jet.com, StackedNutrtion.com, RonnieColemanNutrition.com, RevNutrition.com and Go4ItNutrition.com. ...
(Date:9/19/2017)... Glen Cove, New York (PRWEB) , ... September 19, 2017 , ... ... that its dentist, Dr. Andrew Sami, was recently named one of the best dentists ... the leading lifestyle magazine for dental professionals nationwide. Every fall, the magazine features the ...
(Date:9/19/2017)... (PRWEB) , ... September 19, 2017 , ... ... update their mobile app offering. The new app provides a modern design with ... the overall shopping experience. , The fred’s Pharmacy mobile app includes hundreds of ...
(Date:9/18/2017)... ... September 19, 2017 , ... Food ... market , expected to grow at the fastest CAGR of 9.1% over the ... Food Protein Trends & Growth Opportunities in Asia’ – highlighting alternative proteins, sustainable ...
(Date:9/18/2017)... ... September 18, 2017 , ... Allegheny Health Network’s (AHN) string of standout ... fifth consecutive year, AHN earned more honors than any health care provider in the ... dinner Sept. 14 at the Omni William Penn Hotel in downtown Pittsburgh included:, ...
Breaking Medicine News(10 mins):
(Date:9/5/2017)... Oramed Pharmaceuticals Inc. (NASDAQ: ORMP ) (TASE: ... on the development of oral drug delivery systems, announced ... the U.S. Food and Drug Administration (FDA) regarding ORMD-0801, ... At the meeting, the FDA gave ... ORMD-0801, would be a Biologics License Application (BLA).  Such ...
(Date:9/1/2017)... , Sept. 1, 2017  Bayer will present the latest ... Society for Medical Oncology (ESMO) 2017 Congress, September 8-12 in ... include new preclinical and clinical data on Bayer,s marketed portfolio ... pipeline projects. "We ... minds in cancer research at ESMO," said Carsten Brunn ...
(Date:8/29/2017)... , Aug. 29, 2017 In a ... security, and regulatory compliance for veterinary practices of all ... a new partnership that makes TITAN,s expertise in physical ... available to Cubex,s clients nationally. ... buying and handling controlled substances is at risk today," ...
Breaking Medicine Technology: