Navigation Links
Lab-grown, virus-free stem cells repair retinal tissue in mice
Date:1/23/2014

Investigators at Johns Hopkins report they have developed human induced-pluripotent stem cells (iPSCs) capable of repairing damaged retinal vascular tissue in mice. The stem cells, derived from human umbilical cord-blood and coaxed into an embryonic-like state, were grown without the conventional use of viruses, which can mutate genes and initiate cancers, according to the scientists. Their safer method of growing the cells has drawn increased support among scientists, they say, and paves the way for a stem cell bank of cord-blood derived iPSCs to advance regenerative medicine research.

In a report published Jan. 20 in the journal Circulation, stem cell biologist Elias Zambidis, M.D., Ph.D., and his colleagues describe laboratory experiments with these non-viral, human retinal iPSCs, created using the virus-free method Zambidis first reported in 2011.

"We began with stem cells taken from cord-blood, which have fewer acquired mutations and little, if any, epigenetic memory, which cells accumulate as time goes on," says Zambidis, associate professor of oncology and pediatrics at the Johns Hopkins Institute for Cell Engineering and the Kimmel Cancer Center. The scientists converted these cells to a status last experienced when they were part of six-day-old embryos.

Instead of using viruses to deliver a gene package to the cells to turn on processes that convert the cells back to stem cell states, Zambidis and his team used plasmids, rings of DNA that replicate briefly inside cells and then degrade.

Next, the scientists identified high-quality, multipotent, vascular stem cells generated from these iPSC that can make a type of blood vessel-rich tissue necessary for repairing retinal and other human material. They identified these cells by looking for cell surface proteins called CD31 and CD146. Zambidis says that they were able to create twice as many well-functioning vascular stem cells as compared with iPSCs made with other methods, and, "more importantly these cells engrafted and integrated into functioning blood vessels in damaged mouse retina."

Working with Gerard Lutty, Ph.D., and his team at Johns Hopkins' Wilmer Eye Institute, Zambidis' team injected the newly derived iPSCs into mice with damaged retinas, the light-sensitive part of the eyeball. Injections were given in the eye, the sinus cavity near the eye or into a tail vein. When the scientists took images of the mice retinas, they found that the iPSCs, regardless of injection location, engrafted and repaired blood vessel structures in the retina.

"The blood vessels enlarged like a balloon in each of the locations where the iPSCs engrafted," says Zambidis. The scientists said their cord blood-derived iPSCs compared very well with the ability of human embryonic-derived iPSCs to repair retinal damage.

Zambidis says there are plans to conduct additional experiments of their cells in diabetic rats, whose conditions more closely resemble human vascular damage to the retina than the mouse model used for the current study, he says.

With mounting requests from other laboratories, Zambidis says he frequently shares his cord blood-derived iPSC with other scientists. "The popular belief that iPSCs therapies need to be specific to individual patients may not be the case," says Zambidis. He points to recent success of partially matched bone marrow transplants in humans, shown to be equally as effective as fully matched transplants.

"Support is growing for building a large bank of iPSCs that scientists around the world can access," says Zambidis, although large resources and intense quality- control would be needed for such a feat. However, Japanese scientists led by stem-cell pioneer Shinya Yamanaka are doing exactly that, he says, creating a bank of stem cells derived from cord-blood samples from Japanese blood banks.


'/>"/>

Contact: Vanessa Wasta
wasta@jhmi.edu
410-614-2916
Johns Hopkins Medicine
Source:Eurekalert

Related medicine news :

1. Insulin-producing beta cells from stem cells
2. Tracing unique cells with mathematics
3. Number of cancer stem cells might not predict outcome in HPV-related oral cancers
4. Common blood cancer may be initiated by single mutation in bone cells
5. Not just clean but spotless -- Researchers show how cells tidy up
6. Stem Cells Could Prove Effective in Treating Brittle Bone Disease
7. Keeping stem cells pluripotent
8. Designer swiss-army-knife molecule captures RNA in single cells in their natural tissue environment
9. Study: Autophagy predicts which cancer cells live and die when faced with anti-cancer drugs
10. Platinum Soursop: The Natural Health Supplement Helps to Improve Cells Functions & Supports Immune System Now with 1 Extra Bottle Offer
11. NIH-created toxin can kill HIV-infected cells that persist despite treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/5/2016)... ... ... Textile Exchange is excited to announce the release of the second ... is an important opportunity for interested parties to give input into the standard, and ... The RWS is intended to be a global benchmark for animal welfare and land ...
(Date:5/5/2016)... ... May 05, 2016 , ... Einstein Medical is proud ... cover the latest news and commentary at the 2016 ASCRS/ASOA Symposium and Congress, which ... American Society of Cataract and Refractive Surgery and the American Society of Ophthalmic Administrations ...
(Date:5/5/2016)... ... , ... TLC Laser Eye Centers announced today that it has acquired Gordon ... be called “Gordon Schanzlin New Vision Institute, a TLC Laser Eye Center.” , ... ensure that patients continue to receive the highest quality of care. In addition, all ...
(Date:5/5/2016)... ... May 05, 2016 , ... Spring ... results with IVF and with egg freezing, today announced the grand opening of ... highest quality fertility care, Spring Fertility offers both fertility preservation (egg, sperm, embryo, ...
(Date:5/5/2016)... (PRWEB) , ... May 05, ... ... and hands-on exercises, the Wharton Seminars for Business Journalists , led ... understanding of key business and economic issues.  This one-day program at the ...
Breaking Medicine News(10 mins):
(Date:5/5/2016)... Research and Markets has announced the addition of ...  report to their offering.  ,     (Logo: http://photos.prnewswire.com/prnh/20160330/349511LOGO ... Emission Tomography (PET) scanner and cyclotron install base in ... the current known number of PET scanners and cyclotrons ... also contains a detailed breakdown of this equipment by ...
(Date:5/5/2016)... 5, 2016 Research ... "Europe Systemic Lupus Erythematosus Market and Competitive ... offering.       (Logo: http://photos.prnewswire.com/prnh/20160330/349511LOGO ... Europe Systemic Lupus Erythematosus Market and Competitive ... into Systemic Lupus Erythematosus pipeline products, Systemic ...
(Date:5/4/2016)... , May 4, 2016 According ... Development, Growth and Demand Forecast to 2022 - Industry Insights ... (Hospital, Diagnostic Center and Others)" by P&S Market Research, the ... million in 2015, and it is expected to grow at ... the high slice type segment is expected to witness the ...
Breaking Medicine Technology: