Reduced expression of clusterin in a mouse model of neuroblastoma led to an increased number of tumors compared with control animals.
The role of clusterin in cancer has been unclear. Clusterin may affect processes related to cancer, including programmed cell death, motility, and inflammation.
In the current study, Arturo Sala, Ph.D., of the University College London Institute of Child Health, and colleagues used molecular techniques to increase or decrease expression of clusterin, MYCN, and a group of microRNAs named miR-17-19. MYCN and miR-17-19 microRNAs may regulate clusterin. The researchers assessed the impact of these changes in neuroblastoma cell lines and in animal models of neuroblastoma.
Increased expression of MYCN and miR-17-19 appeared to decrease the expression of clusterin, which was associated with increased tumor growth and metastasis. Increased clusterin expression was associated with reduced tumor growth and metastasis.
"We have provided, to our knowledge, the first evidence to show that clusterin is a tumor suppressor gene that is negatively regulated by the proto-oncogene MYCN," the authors write. "Mice with a disrupted clusterin gene developed more neuroblastomas than mice with a normal clusterin gene."
Contact:: Stephen Cox, Coxs@gosh.nhs.uk, +44(0) 20 7239 3125
Gene Expression Ratio Test Predicts Outcome in Mesothelioma Patients Treated with Surgery
A four-gene expression ratio test prospectively distinguished mesothelioma patients who had a statistically significant longer overall survival from those who had shorter survival in a single-institution study.
There are few effective treatment options available for patients with malignant pleural mesothelioma other than surgery. However, not all patients appear to derive benefit from surgery. Rapha
|Contact: Steve Graff|
Journal of the National Cancer Institute