Navigation Links
International Scientists Secure Quality in Molecular Diagnostics
Date:3/31/2009

Consensus Guideline Reached For Quantitative Polymerase Chain Reaction

SALT LAKE CITY, March 31 /PRNewswire/ -- ARUP Laboratories and the American Association for Clinical Chemistry (AACC) announced today that a consensus guideline for a key laboratory method called qPCR (or quantitative polymerase chain reaction) was published by a group of international scientists representing the medical and research fields.

qPCR is extensively used in diagnostic testing, from pathogen detection to cancer prognosis, and new studies using qPCR are published at an ever-increasing rate. The consensus guideline, the first of its kind for this technique, provides a minimum set of information that researchers must provide in order for their qPCR data to be considered for publication. The goal is to increase the transparency and quality of studies, so that experiments using qPCR can be accurately reproduced by others, and the scientific community can assess the quality and validity of those studies more readily.

Authors of this guideline, including Professor Stephen A. Bustin at the Institute of Cell and Molecular Science, Queen Mary, University of London, recognized that there was a widespread problem in how qPCR data were reported and interpreted. "The majority of published qPCR studies do not provide sufficient experimental detail, and scientists reading them have a hard time deciding if the conclusions are valid. For instance, this has contributed to the erroneous reporting of the association between child MMR vaccination and autism," said Dr. Bustin. When analyzed correctly, the results proved to be due to poorly implemented experimental technique. Another example is a study on plant genetics that was published in the journal Science as the "breakthrough of the year" in 2005. Two years later, this study had to be retracted because of poorly implemented qPCR.

The guideline includes a checklist of essential and desirable steps that should be followed when using qPCR. If followed appropriately, authors should be able to design and report qPCR experiments with greater inherent value, and journal reviewers, editors, and laboratory managers will be able to evaluate the technical quality of the published data against an established yardstick. Most importantly, adherence to this guideline should facilitate the publication of results that will be factually reliable.

"This guideline is important because many of these studies become the basis of new molecular diagnostic tests. They have to be reliable," commented co-author Dr. Carl Wittwer, professor at the University of Utah and medical director at ARUP Laboratories.

"This guideline was created by scientists from five countries who are very knowledgeable in qPCR. The guideline should help us evaluate future qPCR studies that are submitted to our journal," remarked Dr. Nader Rifai, editor of the journal Clinical Chemistry, which will publish this guideline in its April 2009 issue. Full access to the guideline is available online at: www.clinchem.org/cgi/content/full/55/4/611.

BACKGROUND

About quantitative PCR (qPCR):

qPCR is an extension of the polymerase chain reaction invented in 1986 by Kary Mullis, which revolutionized biological research and resulted in him receiving the 1993 Nobel Prize in Chemistry. qPCR accurately measures DNA by turning a single copy of DNA into millions of copies in less than an hour. Its simplicity made this technique a quintessential method for scientific research, medical and forensic analysis, as well as for biodefense and food safety applications.

More about the guideline and related information:

  1. http://www.rdml.org/miqe/
  2. RDML: structured language and reporting guidelines for real-time quantitative PCR data. Nucleic Acids Res. 2009;Feb 17.

About AACC:

The American Association for Clinical Chemistry (AACC) is a leading professional society dedicated to improving healthcare through laboratory medicine. Its over 9,000 members are clinical laboratory professionals, physicians, research scientists, and others involved in developing tests and directing laboratory operations. AACC brings this community together with programs that advance knowledge, expertise, and innovation. For more information about this topic, or about AACC and its programs and publications, please contact Peter Patterson on the above telephone numbers or at ppatterson@aacc.org.

About ARUP Laboratories:

ARUP Laboratories is a national clinical and anatomic pathology reference laboratory and an enterprise of the University of Utah and its Department of Pathology. With more than 2,400 employees, ARUP offers in excess of 2,000 tests and test combinations for patients throughout the country. Additionally, ARUP's Institute for Clinical and Experimental Pathology(R) is a worldwide leader in innovative laboratory research and development. Visit ARUP on the Web at www.aruplab.com.


'/>"/>
SOURCE ARUP Laboratories
Copyright©2009 PR Newswire.
All rights reserved

Related medicine news :

1. International Liver Congress 2009
2. Lighthouse International Expands Vision Health Services for All New Yorkers
3. Five Research Studies at International SRCD Meeting in Denver Use Technology from Boulder Foundation
4. Chicago School of Professional Psychology Announces New Ph.D. in International Psychology
5. Chiltern and EPS International Hold a Joint Seminar in Tokyo, Japan
6. Garvan to play a role in International Cancer Genome Consortium
7. International Pooper Scooper Week - April 1-7
8. AmeriCares and International Medical Corps Partner to Bring Lifesaving Aid to Chad
9. Supermodel Izabel Goulart Partners With Diabetes Research Institute Foundation as International Ambassador
10. Wound Management Technologies Exhibits at Premier International Diabetic Foot Conference DFCon 09
11. PRA International Speaking Event: Beyond Global Feasibility
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/20/2017)... ... January 20, 2017 , ... Michael and Betsy Brauser celebrated 5 years ... Betsy, the clinical trial has been life-saving as she has been on the ... Betsy Brauser was diagnosed with ovarian cancer in 2009. She underwent standard chemotherapy but ...
(Date:1/20/2017)... ... ... Magdalene: Grace is Greater than Sin”: a unique and memorable piece of literature ... Grace is Greater than Sin” is the creation of published author, Brenda Roberts, a ... had little knowledge of the female characters portrayed in the Holy Bible. , ...
(Date:1/20/2017)... ... January 20, 2017 , ... “I Forgive You”: a fine examination of how ... You” is the creation of published author, Stephen Miller, who, for over ten long years ... given to him. Born in Trinidad and Tobago, he has been serving the Lord for ...
(Date:1/20/2017)... ... ... Christmas:” a beautiful and enchanting tale that teaches children the true meaning of Christmas. “Journey ... Oklahoma City, and a devoted woman of faith. , “Becoming a parent changes you. ... of my mind for years, but actually doing it might have been a while in ...
(Date:1/20/2017)... ... , ... “God's Miracle Man: Against All Odds”: an inspiring affirmation of ... Keith C. A. Tucker, son of Minister Delores Pinnock and a Jamaican native who ... Hardy , “While sitting up in bed, I felt a pounding headache. It was ...
Breaking Medicine News(10 mins):
(Date:1/20/2017)... 20, 2017 Research and Markets has ... (by Devices, Implant Types, OTC Amplifiers, Diagnostic Instruments), Sales Volume, ... offering. ... Global Hearing Aids Market Sales Volume, Company Analysis and Forecast ... hearing aid industry. The growing prevalence of hearing ...
(Date:1/20/2017)... 20, 2017  Palladian Health, a leading provider ... launch of an opioid management program which assists ... and helps stem the growing tide of dependence ... treat chronic non-cancer pain (back pain, neck pain, ... lack of evidence regarding long-term effectiveness. ...
(Date:1/19/2017)... Germany Cataract Surgery Devices Market Outlook ... Cataract Surgery Devices Market Outlook to 2022", provides key ... The report provides value, in millions of US dollars, ... segements - Phacoemulsification Equipment and Ophthalmic Viscoelastic Devices (OVD). ... shares data for each of these market segements, and ...
Breaking Medicine Technology: