Navigation Links
International Scientists Secure Quality in Molecular Diagnostics

Consensus Guideline Reached For Quantitative Polymerase Chain Reaction

SALT LAKE CITY, March 31 /PRNewswire/ -- ARUP Laboratories and the American Association for Clinical Chemistry (AACC) announced today that a consensus guideline for a key laboratory method called qPCR (or quantitative polymerase chain reaction) was published by a group of international scientists representing the medical and research fields.

qPCR is extensively used in diagnostic testing, from pathogen detection to cancer prognosis, and new studies using qPCR are published at an ever-increasing rate. The consensus guideline, the first of its kind for this technique, provides a minimum set of information that researchers must provide in order for their qPCR data to be considered for publication. The goal is to increase the transparency and quality of studies, so that experiments using qPCR can be accurately reproduced by others, and the scientific community can assess the quality and validity of those studies more readily.

Authors of this guideline, including Professor Stephen A. Bustin at the Institute of Cell and Molecular Science, Queen Mary, University of London, recognized that there was a widespread problem in how qPCR data were reported and interpreted. "The majority of published qPCR studies do not provide sufficient experimental detail, and scientists reading them have a hard time deciding if the conclusions are valid. For instance, this has contributed to the erroneous reporting of the association between child MMR vaccination and autism," said Dr. Bustin. When analyzed correctly, the results proved to be due to poorly implemented experimental technique. Another example is a study on plant genetics that was published in the journal Science as the "breakthrough of the year" in 2005. Two years later, this study had to be retracted because of poorly implemented qPCR.

The guideline includes a checklist of essential and desirable steps that should be followed when using qPCR. If followed appropriately, authors should be able to design and report qPCR experiments with greater inherent value, and journal reviewers, editors, and laboratory managers will be able to evaluate the technical quality of the published data against an established yardstick. Most importantly, adherence to this guideline should facilitate the publication of results that will be factually reliable.

"This guideline is important because many of these studies become the basis of new molecular diagnostic tests. They have to be reliable," commented co-author Dr. Carl Wittwer, professor at the University of Utah and medical director at ARUP Laboratories.

"This guideline was created by scientists from five countries who are very knowledgeable in qPCR. The guideline should help us evaluate future qPCR studies that are submitted to our journal," remarked Dr. Nader Rifai, editor of the journal Clinical Chemistry, which will publish this guideline in its April 2009 issue. Full access to the guideline is available online at:


About quantitative PCR (qPCR):

qPCR is an extension of the polymerase chain reaction invented in 1986 by Kary Mullis, which revolutionized biological research and resulted in him receiving the 1993 Nobel Prize in Chemistry. qPCR accurately measures DNA by turning a single copy of DNA into millions of copies in less than an hour. Its simplicity made this technique a quintessential method for scientific research, medical and forensic analysis, as well as for biodefense and food safety applications.

More about the guideline and related information:

  2. RDML: structured language and reporting guidelines for real-time quantitative PCR data. Nucleic Acids Res. 2009;Feb 17.

About AACC:

The American Association for Clinical Chemistry (AACC) is a leading professional society dedicated to improving healthcare through laboratory medicine. Its over 9,000 members are clinical laboratory professionals, physicians, research scientists, and others involved in developing tests and directing laboratory operations. AACC brings this community together with programs that advance knowledge, expertise, and innovation. For more information about this topic, or about AACC and its programs and publications, please contact Peter Patterson on the above telephone numbers or at

About ARUP Laboratories:

ARUP Laboratories is a national clinical and anatomic pathology reference laboratory and an enterprise of the University of Utah and its Department of Pathology. With more than 2,400 employees, ARUP offers in excess of 2,000 tests and test combinations for patients throughout the country. Additionally, ARUP's Institute for Clinical and Experimental Pathology(R) is a worldwide leader in innovative laboratory research and development. Visit ARUP on the Web at

SOURCE ARUP Laboratories
Copyright©2009 PR Newswire.
All rights reserved

Related medicine news :

1. International Liver Congress 2009
2. Lighthouse International Expands Vision Health Services for All New Yorkers
3. Five Research Studies at International SRCD Meeting in Denver Use Technology from Boulder Foundation
4. Chicago School of Professional Psychology Announces New Ph.D. in International Psychology
5. Chiltern and EPS International Hold a Joint Seminar in Tokyo, Japan
6. Garvan to play a role in International Cancer Genome Consortium
7. International Pooper Scooper Week - April 1-7
8. AmeriCares and International Medical Corps Partner to Bring Lifesaving Aid to Chad
9. Supermodel Izabel Goulart Partners With Diabetes Research Institute Foundation as International Ambassador
10. Wound Management Technologies Exhibits at Premier International Diabetic Foot Conference DFCon 09
11. PRA International Speaking Event: Beyond Global Feasibility
Post Your Comments:
(Date:11/25/2015)... ... November 25, 2015 , ... Today, Mothers Against Drunk Driving ... dropped below 10,000 for the first time since 2011. In 2014, there were 9,967 ... data released by the National Highway Traffic Safety Administration (NHTSA), 32,675 people were killed ...
(Date:11/25/2015)... ... November 25, 2015 , ... "When I underwent breast reconstruction ... incredibly uncomfortable," said an inventor from Bronx, N.Y. "In order to meet my ... RECOVERY BRA for added comfort and support. The bra is easier to put ...
(Date:11/25/2015)... ... 25, 2015 , ... Many people know of the common symptoms of low ... dry skin. But many people who find their cholesterol levels and weight are creeping ... thyroid, especially if they don’t have any of the other symptoms. , Thyroid hormone ...
(Date:11/25/2015)... ... November 25, 2015 , ... Students and parents have something ... winners of the Create Real Impact awards. California Casualty is proud to ... the tide of distracted and reckless driving, the number one killer of young drivers. ...
(Date:11/24/2015)... (PRWEB) , ... November 24, 2015 , ... ... certified to offer their patients the many benefits of the revolutionary BIOLASE WaterLase ... sharp cutting and scraping tools traditionally used by a dentist in Gettysburg, ...
Breaking Medicine News(10 mins):
(Date:11/24/2015)... 24, 2015   HeartWare International, Inc . (NASDAQ: ... circulatory support technologies that are revolutionizing the treatment of ... Executive Officer Doug Godshall is scheduled to ... Healthcare Conference on December 1, 2015 at 3:00 p.m. ... New York . ...
(Date:11/24/2015)... LAUSANNE and BERN, Switzerland ... SA, the ARTORG Center for Biomedical Engineering Research of ... and the Division of Endocrinology, Diabetes and Clinical Nutrition ... announce the start of an exclusive collaboration to develop ... control algorithm for the personalised delivery of insulin for ...
(Date:11/24/2015)... 2015 F1000Workspace - a research ... it was launched just six months ago. --> ... platform for scientists - since it was launched just six ... loaded on to F1000Workspace - a research collaboration, ... was launched just six months ago. --> ...
Breaking Medicine Technology: