Navigation Links
Improved identification of war wound infections promises more successful treatment
Date:5/29/2014

War wounds that heal successfully frequently contain different microbial species from those that heal poorly, according to a paper published ahead of print in the Journal of Clinical Microbiology. These and other findings have important implications for improving wound healing, says first author Nicholas Be of Lawrence Livermore National Laboratory, Livermore, California.

The problem the researchers were addressing is that culture-based identification, which has been used to assay war wound infections, misses the many species that are difficult or impossible to culture. But Be and his collaborators posited that using microarrays and whole genome sequencing to detect microbial species in wound samples would reveal infections caused by microbes that cannot be cultured, as these molecular methods can detect all species for which reference DNA is available.

"We also hypothesized that different microorganisms could be associated with successful or unsuccessful healing, and we felt that this information could be used for guiding medical treatment," says Be.

In the study, the investigators found that genetic sequences from certain bacteria, including Pseudomonas species and Acinetobacter baumannii, were frequently observed in wounds that failed to heal, while bacteria typically associated with the gastrointestinal system, such as E. coli and Bacteroides species, were found in wounds that did heal successfully.

"This surprising finding further emphasizes the need for specific molecular detection," says Be. "We also observed via whole genome sequencing that the complex microbial populations present in wounds vary between patients and change over time in a single patient, further emphasizing the need for personalized treatment of individual wounds."

The investigators examined 124 wound samples from 61 wounds in 44 patients injured in combat in Iraq and Afghanistan. They used a microbial detection microarray developed at Lawrence Livermore National Laboratory, which contains DNA probes capable of detecting any microorganisms that have previously been sequenced.

"This represents a cost-effective, high-throughput platform for analysis of wound infections," says Be. A subset of samples was also subjected to whole genome sequencing.

"Information on the presence of specific bacteria that more significantly affect the success of the healing response could guide therapy and allow for more accurate prediction of outcome," says Be. "More effective, specific, and timely diagnosis of infection would improve treatment, accelerate rehabilitation, and decrease the length of hospital stays."

The manuscript can be found online at http://bit.ly/asmtip0514i. The final version of the article is scheduled for the July 2014 issue of the Journal of Clinical Microbiology.


'/>"/>

Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology
Source:Eurekalert

Related medicine news :

1. Despite economic blows, infant health has improved among US poor
2. E-cigarette use for quitting smoking is associated with improved success rates
3. Donor livers preserved and improved with room-temperature perfusion system
4. Aspirin use appears linked with improved survival after colon cancer diagnosis
5. MATH, HPV status in HNSCC patients effective markers of improved patient outcome
6. Researchers identify possible explanation for link between exercise & improved prostate cancer outcomes
7. Latest Research Shows Improved Long-Term Memory with Same Caffeine Level as 5-hour ENERGY® Shots
8. Personal health record associated with improved medication adherence
9. CEO of Simply G Media GJ Reynolds Discussed His Launch Of The New and Improved SimplyG.com Website on The BeachLifestyle Radio Show.
10. Improved Method of Securing IV Lines Invented by InventHelp Client (BIR-2159)
11. InventHelp Inventor Designs an Improved Sanitary Pad (PND-4272)
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/2/2016)... ... December 02, 2016 , ... Advanced Inc., a leading provider of travel therapy ... MBA to serve as Advanced Inc.’s Chief Financial Officer, effective December 1, 2016. Jason ... extensive financial and operational leadership experience to Advanced Inc. He began his career in ...
(Date:12/2/2016)... ... December 02, 2016 , ... With the number of ... of an injury, patients must find the one that works for them. When an ... created a machine that worked and decided to share it with others. , He ...
(Date:12/2/2016)... ... December 02, 2016 , ... CloudLIMS.com, a class-leading provider of ... Lite. CloudLIMS Lite helps biobanks, clinical, research and testing laboratories keep track of ... new version is a faster and a more efficient product, allowing batch processing ...
(Date:12/2/2016)... ... 2016 , ... Yisrayl Hawkins, at The House of Yahweh in Abilene, Texas, ... Bible Prophecy. Yisrayl says this generation, known as the Last Generation, started in 1934 ... details line up exactly with Bible Prophecy – a protected way for those who ...
(Date:12/2/2016)... ... December 02, 2016 , ... ... & Author of Type 2 Diabetes: The Owner’s Manual, http://realtimepressrelease.com/press-releases-tagged-with/daryl-wein ... Hour Power of Water, Global Climate Change and Your Health radio program syndicated ...
Breaking Medicine News(10 mins):
(Date:12/2/2016)... , December 2, 2016 ... Awards Committee honored excellence in research, development and innovation ... The gala dinner was held in the presence of ... the Russian Federation , Natalia Sanina, ... Mikhail Murashko , Head of Roszdravnadzor, National Service ...
(Date:12/2/2016)... 2, 2016  Maxor National Pharmacy Services, LLC ("Maxor"), today ... Inc. ("PSI").  The combination of Texas -based ... -based PSI bring together both company,s clinical expertise and high-touch ... an industry-leading specialty pharmacy. About Maxor ... , , ...
(Date:12/2/2016)... Dec. 2, 2016 Boston Scientific Corporation (NYSE: ... to acquire certain manufacturing assets and capabilities of the ... advanced biological tissue business, as well as a 15% ... million in cash. The Neovasc advanced biological tissue business ... Boston Scientific Lotus™ Valve System. * Upon completion ...
Breaking Medicine Technology: