Navigation Links
Human umbilical cord blood cell co-culture supports embryonic stem cell expansion
Date:11/15/2012

Putnam Valley, NY. (Nov. 15, 2012) Researchers in Taiwan have developed a "safe, feasible and robust co-culture system" supplied by human umbilical cord mensenchymal stem cells (HUCMSCs) to feed the sustained culture used for human embryonic stem cell (hESC) expansion prior to cell transplantation. The co-culture, said the researchers, "appears to eliminate the most feared characteristic of transplanted hESCs," which is their propensity to form tumors.

The study, published in the current issue of CELL TRANSPLANTATION, is now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/.

"Embryonic stem cells have pluripotent potential," said study co-author Dr. Tang-Yuan Chu of the Buddhist Tzu Chi General Hospital at Tzu Chi University in Hualien, Taiwan. "The sustained maintenance of hESCs depends on a co-culture with an animal based "feeder" that can create the risk for transmitting nonhuman materials and unknown pathogens. To solve this problem, human tissues have been used as feeders."

The expansion of pluripotent hESCs traditionally requires a feeder culture, meaning that a variety of animal and human tissues have been used in feeder cultures.

The researchers note that while hESCs have been successfully co-cultured using human fetal muscle and skin cells, adult fallopian tube epithelial cells, foreskin cells, and bone marrow stem cells, their study used hUCMScs to create a co-culture. They said that using hUCMSCs as a source feeder has several advantages, including their wide availability, ease of handling and low immunogenicity.

However, according to the researchers, when using various mouse or primate tissues, and even when using human tissues for co-cultures, tumor-like formations called "teratomas" - growths containing tissues belonging to all three germ layers - often form. Although undesirable, teratomas have been used as a marker for the ability of stem cells to be able to develop pluripotency.

This study demonstrated that pluripotency development need not necessarily be teratoma forming.

"We suggest that the feeder we developed from hUCMSCs may support the transition of hESCs that does not grow teratomas because, unlike tissue sources for other feeders, by using hUCMSCs we did not use material comprised of all three germ layers," said the researchers.

"In addition to eliminating teratomas, the proposed system also significantly reduces the workload involved in the preparation of new feeder lines," they concluded.


'/>"/>

Contact: Robert Miranda
cogcomm@aol.com
Cell Transplantation Center of Excellence for Aging and Brain Repair
Source:Eurekalert

Related medicine news :

1. Sociology, economics researchers receive grant to study development across the human lifespan
2. Gut Bacteria Often Similar in Humans, Chimps: Study
3. First trial in humans of minicells: A completely new way of delivering anti-cancer drugs
4. Testing pain killers on humans could save money and speed the arrival of new drugs
5. New, improved mouse model of human Alzheimers may enable drug discovery
6. Spread of human melanoma cells in mice correlates with clinical outcomes in patients
7. First gene therapy study in human salivary gland shows promise
8. Clinical trials for first-ever human hookworm vaccine advance
9. Bigger human genome pool uncovers rarer variants
10. A Viagra follow-up? Drug used to treat glaucoma actually grows human hair
11. Genes of Africas Khoe-San Peoples Give Clues to Human Evolution
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... , ... October 13, 2017 , ... “The Journey: From ... every danger possible to save lost souls in the Philippines. “The Journey: From the ... is a dedicated teacher of the Bible. She has taught all ages and currently ...
(Date:10/12/2017)... ... , ... Planet Fitness, one of the largest and fastest growing franchisors and ... flagship location in Covington, LA at 401 N. U.S. Highway 190, in January of ... Depot in the Holiday Square shopping center. Its location allows it to serve both ...
(Date:10/12/2017)... ... 12, 2017 , ... Asante, a nationally recognized health system ... their existing home health joint venture through an agreement, effective October 1, 2017, ... venture home health company with Asante, delivering clinically integrated care, for the past ...
(Date:10/12/2017)... ... October 12, 2017 , ... Leading pediatric oncology experts ... Washington, D.C., for the 49th Congress of the International Society of Paediatric Oncology ... of the Center for Cancer and Blood Disorders at Children’s National, and ...
(Date:10/12/2017)... ... October 12, 2017 , ... In the United States, ... In some states—like New York, New Jersey, Massachusetts, Texas, Virginia, Connecticut, and California—the ... have extremely low property-tax rates, which contributes to the relatively lower cost of ...
Breaking Medicine News(10 mins):
(Date:9/18/2017)... KALAMAZOO, Mich. , Sept. 18, 2017  PMD ... OptiMed Specialty Pharmacy of Kalamazoo, Mich. ... strategic hub service that expedites and streamlines patient and ... Spiro PD 2.0, and wellness management services.  ... medical device used to measure lung function for a ...
(Date:9/12/2017)... , Sept. 12, 2017   EcoVadis , the leading platform for ... the first annual edition of its Global CSR Risk and Performance Index. ... by EcoVadis, based on Scorecard Ratings that analyzed nearly 800,000 data points ... ... ...
(Date:9/9/2017)... ... ... Monday, September 18 th .The Brain Tumor Foundation (BTF) begins a week-long ... BTF,s Mobile MRI Unit – a 70 Ft. ... brings its nationwide initiative, the Road to Early Detection Sponsor-A-City Campaign to ...
Breaking Medicine Technology: