Navigation Links
Human umbilical cord blood cell co-culture supports embryonic stem cell expansion

Putnam Valley, NY. (Nov. 15, 2012) Researchers in Taiwan have developed a "safe, feasible and robust co-culture system" supplied by human umbilical cord mensenchymal stem cells (HUCMSCs) to feed the sustained culture used for human embryonic stem cell (hESC) expansion prior to cell transplantation. The co-culture, said the researchers, "appears to eliminate the most feared characteristic of transplanted hESCs," which is their propensity to form tumors.

The study, published in the current issue of CELL TRANSPLANTATION, is now freely available on-line at

"Embryonic stem cells have pluripotent potential," said study co-author Dr. Tang-Yuan Chu of the Buddhist Tzu Chi General Hospital at Tzu Chi University in Hualien, Taiwan. "The sustained maintenance of hESCs depends on a co-culture with an animal based "feeder" that can create the risk for transmitting nonhuman materials and unknown pathogens. To solve this problem, human tissues have been used as feeders."

The expansion of pluripotent hESCs traditionally requires a feeder culture, meaning that a variety of animal and human tissues have been used in feeder cultures.

The researchers note that while hESCs have been successfully co-cultured using human fetal muscle and skin cells, adult fallopian tube epithelial cells, foreskin cells, and bone marrow stem cells, their study used hUCMScs to create a co-culture. They said that using hUCMSCs as a source feeder has several advantages, including their wide availability, ease of handling and low immunogenicity.

However, according to the researchers, when using various mouse or primate tissues, and even when using human tissues for co-cultures, tumor-like formations called "teratomas" - growths containing tissues belonging to all three germ layers - often form. Although undesirable, teratomas have been used as a marker for the ability of stem cells to be able to develop pluripotency.

This study demonstrated that pluripotency development need not necessarily be teratoma forming.

"We suggest that the feeder we developed from hUCMSCs may support the transition of hESCs that does not grow teratomas because, unlike tissue sources for other feeders, by using hUCMSCs we did not use material comprised of all three germ layers," said the researchers.

"In addition to eliminating teratomas, the proposed system also significantly reduces the workload involved in the preparation of new feeder lines," they concluded.


Contact: Robert Miranda
Cell Transplantation Center of Excellence for Aging and Brain Repair

Related medicine news :

1. Sociology, economics researchers receive grant to study development across the human lifespan
2. Gut Bacteria Often Similar in Humans, Chimps: Study
3. First trial in humans of minicells: A completely new way of delivering anti-cancer drugs
4. Testing pain killers on humans could save money and speed the arrival of new drugs
5. New, improved mouse model of human Alzheimers may enable drug discovery
6. Spread of human melanoma cells in mice correlates with clinical outcomes in patients
7. First gene therapy study in human salivary gland shows promise
8. Clinical trials for first-ever human hookworm vaccine advance
9. Bigger human genome pool uncovers rarer variants
10. A Viagra follow-up? Drug used to treat glaucoma actually grows human hair
11. Genes of Africas Khoe-San Peoples Give Clues to Human Evolution
Post Your Comments:
(Date:11/25/2015)... ... ... “While riding the bus, I saw a passenger in a wheelchair drenched ... be a convenient and comfortable way to protect them from bad weather, so I ... travel during cold or inclement weather. In doing so, it ensures that the user ...
(Date:11/25/2015)... ... November 25, 2015 , ... Privately owned Contract Development ... expansion of its current state of the art research, development and manufacturing facility ... increase its manufacturing capacity as well as to support its clients’ growing research ...
(Date:11/25/2015)... ... November 25, 2015 , ... Students and parents have something to be thankful ... Create Real Impact awards. California Casualty is proud to support the contest ... distracted and reckless driving, the number one killer of young drivers. , Almost ...
(Date:11/24/2015)... Mississauga, ON (PRWEB) , ... November 24, 2015 , ... ... Deborah Williams without a referral for dental implants at her Mississauga, ON ... qualified and experienced in the placement of dental implants. , Missing teeth can ...
(Date:11/24/2015)... ... November 25, 2015 , ... Catalent ... for drugs, biologics, consumer health and global clinical supply services, today announced that ... Clinical Trial Supply East Asia Conference, to be held at the InterContinental Seoul ...
Breaking Medicine News(10 mins):
(Date:11/24/2015)... and BERN, Switzerland , November ... ARTORG Center for Biomedical Engineering Research of the University ... Division of Endocrinology, Diabetes and Clinical Nutrition of the ... start of an exclusive collaboration to develop a novel ... for the personalised delivery of insulin for diabetic patients ...
(Date:11/24/2015)... FLINT, Mich. , Nov. 24, 2015 Diplomat ... , Senior Vice President of Clinical Services, Education and Human ... (AIS) online webinar, "Oral Oncology Drugs: Health Plan Strategies for ... co-presenting with Beckie Fenrick , a consultant with the ... The webinar will ...
(Date:11/24/2015)... 24, 2015 iRhythm Technologies, Inc. , a leading ... announced that it will participate in the 27th Annual Piper Jaffray ... New York, NY . Kevin King , Chief ... 1, 2015 at 8:50am ET. --> ... . --> . --> iRhythm is ...
Breaking Medicine Technology: