Navigation Links
Gold nanoparticles help target, quantify breast cancer segments in a living cell
Date:4/23/2014

WEST LAFAYETTE, Ind. - Purdue University researchers have developed a way to detect and measure cancer levels in a living cell by using tiny gold particles with tails of synthetic DNA.

A team led by Joseph Irudayaraj, professor of agricultural and biological engineering, used gold nanoparticles to target and bind to fragments of genetic material known as BRCA1 messenger RNA splice variants, which can indicate the presence and stage of breast cancer. The number of these mRNA splice variants in a cell can be determined by examining the specific signal that light produces when it interacts with the gold nanoparticles.

"This is a simple yet sophisticated technique that can be used to detect cancer in a single cell and determine how aggressive it is," said Irudayaraj, who is also the deputy director of the Bindley Bioscience Center. "Being able to quantify these genetic molecules could ultimately help clinicians provide better and more individualized treatment to cancer patients."

The technique also could increase our understanding of cell biology and paves the way for genetic profiling and diagnosis based on a single cell, Irudayaraj said.

BRCA1 is a tumor suppressor gene that can transform a cell into a cancerous type under certain circumstances. Measuring the number of BRCA1 mRNA splice variants in a cell can indicate if the gene is being under-expressed, a possible sign of breast cancer.

But current methods of detecting cancer rely on samples made up of hundreds or thousands of cells and cannot provide detailed information about how genes tied to cancer are being expressed in individual cells.

Irudayaraj and his team are the first to detect and quantify BRCA1 mRNA splice variants - fragments of genetic material that are removed when mRNA is formed - in a single cell. Splice variants can determine the fate of a cell and how specific proteins are expressed. Errors in the splicing process have been linked to a variety of diseases.

"With this method, we are basically able to spot a needle in a haystack - and we can determine if there are five needles in that haystack or if there are 50," he said.

Irudayaraj and his then-graduate research assistant, Kyuwan Lee, who is the first author of the study, adapted common nanotechnology methods to tackle the challenge of pinpointing mRNA splice variants in a living cell. They fabricated gold nanoparticles - more than 1,000 times smaller than the diameter of a human hair - and tagged them with strands of DNA complementary to BRCA1 mRNA splice variants.

When injected into a cell, the nanoparticles attached to either end of mRNA splice variants, forming structures known as dimers - each "like a couple holding hands," Irudayaraj said.

Because dimers give off a unique signal in the presence of light, the researchers could measure the number of dimers by illuminating the cell with a simple light source. The number of dimers corresponded to the number of BRCA1 mRNA splice variants in a cell.

Light behaves differently when it shines on a single gold particle, allowing the researchers to differentiate between dimers and free-floating gold particles.

The researchers used two methods to quantify the dimers: spectroscopy, which measures the way light scatters when it encounters an object, and a colorimetric image on which dimers show as reddish dots while single gold particles appear green.

The technique can quantify mRNA splice variants in a single cell in about 30 minutes.

Irudayaraj is modifying the system to speed up the process so that it can be used in tissue biopsies.

"If we can quantify key mRNA at single cell resolution in a tissue biopsy, that will be very powerful in terms of refining treatment protocols for key diseases," he said.


'/>"/>

Contact: Natalie van Hoose
nvanhoos@purdue.edu
765-496-2050
Purdue University
Source:Eurekalert  

Related medicine news :

1. Clemson researchers develop sticky nanoparticles to fight heart disease
2. Nanoparticles treat muscular dystrophy in mice
3. Nanoparticles to probe mystery sperm defects behind infertility
4. Shape of nanoparticles points the way toward more targeted drugs
5. New gene delivery method: magnetic nanoparticles
6. Shape-shifting nanoparticles flip from sphere to net in response to tumor signal
7. Mayo Clinic: How gold nanoparticles can help fight ovarian cancer
8. New technique can help nanoparticles deliver drug treatments
9. Nanoparticles loaded with bee venom kill HIV
10. Researchers show that lipid nanoparticles are ideal for delivering genes and drugs
11. New electrically-conductive polymer nanoparticles can generate heat to kill colorectal cancer cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Gold nanoparticles help target, quantify breast cancer segments in a living cell
(Date:2/10/2016)... (PRWEB) , ... February 10, 2016 , ... As part ... two webinars in February 2016. Each webinar features a dynamic expert and thoughtful ... benefit their athletes, patients and facilities. Both events are free to attend, but ...
(Date:2/10/2016)... Huntington, NY (PRWEB) , ... February 10, 2016 ... ... web-based compliance tracking solution, The Guard, to associations of medical professionals throughout the ... HIPAA compliance, including security risk assessments, policies and procedures, employee training, regulatory updates, ...
(Date:2/10/2016)... ... February 10, 2016 , ... 10 Best Water is ... leading bottled water brand owners that topped the list as a result of their ... conversion. The premier brand was Tibet 5100, a top notch water company that specializes ...
(Date:2/10/2016)... AZ (PRWEB) , ... February 10, 2016 , ... Ongoing ... the Aging Life Care Association™ (ALCA) to conduct a survey that takes a closer ... experts released today illustrates the prevalence and causes of TBI among the aging population, ...
(Date:2/10/2016)... ... 10, 2016 , ... Gout is like no other joint pain. It strikes ... with intense swelling and redness. It is triggered by the crystallization of uric acid ... the most susceptible, according to the February 2016 issue of Harvard Men's Health Watch. ...
Breaking Medicine News(10 mins):
(Date:2/10/2016)... LOS ANGELES , Feb. 10, 2016  Oxis ... blood cancer drug, OXS-1550, was described as a "clinical ... with the drug went into complete cancer remission. ... the University of Minnesota Masonic Cancer Center. ... the University of Minnesota Masonic Cancer Center. ...
(Date:2/10/2016)... Feb. 10, 2016  LexisNexis® Risk Solutions, a ... announced the launch of LexisNexis Provider Performance ... helps improve and optimize the quality and efficiency ... using severity-adjusted scores. By measuring provider performance through ... to deliver better outcomes, improve the patient experience ...
(Date:2/10/2016)... and OR AKIVA, Israel ... Regentis Biomaterials Ltd., a leader in the field ... D investment round on February 5, 2016. The $15 ... ("Haisco"), a leading Chinese pharmaceutical manufacturer, and was ... Vitalife Partners, Generali Financial Holdings and both the Technion ...
Breaking Medicine Technology: