Navigation Links
Gladstone scientists develop technique to decipher the dormant AIDS virus concealed in cells
Date:9/11/2012

SAN FRANCISCO, CASeptember 11, 2012Scientists at the Gladstone Institutes have gotten us one step closer to understanding and overcoming one of the least-understood mechanisms of HIV infectionby devising a method to precisely track the life cycle of individual cells infected with HIV, the virus that causes AIDS.

In a paper being published online today in Lab on a Chip, the laboratory of Gladstone Investigator Leor Weinberger, PhD, announced the development of a device that can pinpoint and track HIV inside CD4 T cellsthe type of white blood cell that the AIDS virus targets. This development is particularly important for understanding "HIV latency," a state in which the virus goes dormant after the patient begins standard antiretroviral treatment. Current antiretroviral drugs do not kill HIVthey only keep it at baymeaning that those with HIV must continue a lifetime of drug treatment so as not to develop AIDS. If they discontinue the drugs, the latent virus "wakes up" within just a few weeks and begins an onslaught against the body's immune system.

The breakthrough comes as the AIDS-researcher community is beginning to speak publicly about the possibility of curing HIV/AIDS. Understandingand consequently interruptingHIV latency is a key element in the effort to discover a cure for this devastating disease.

"HIV latency is perhaps the single greatest obstacle to eradicating HIV/AIDS in the 34 million people who live with the disease worldwide," said Dr. Weinberger, who is also an associate professor of biochemistry and biophysics at the University of California, San Francisco (UCSF), with which Gladstone is affiliated. "Existing techniques that try to uncover the cellular and viral mechanisms behind HIV latency are inefficient at studying very rare cellsand cells housing the latent HIV virus are one-in-a-million. Our technique presents a clear path towards understanding how HIV latency is regulated within a single cell, by tracking the individual cells that traditionally had been difficult to monitor."

Singe-cell, time-lapse microscopya state-of-the-art technique that scientists have lately used to track some viral infections and map antibiotic resistance to drugshas not worked for tracking the HIV-infection cycle in CD4 T cells, especially in the latent state. This is because these cells are notoriously evasive. They spontaneously move around, attaching and detaching from their neighbors, making it nearly impossible to monitor individual HIV-infected cells over time.

However, Dr. Weinberger's team devised a clever system that essentially guides and suspends HIV-infected T cells into tiny finger-like channelsreducing their ability to move or detach from their neighbors.

"First, we load the T cells into a small well, allowing them to settle into the bottomwhich is filled with nutrients that keep the cells well-fed and stress-free," explained the paper's lead author Brandon Razooky, a Gladstone and UCSF graduate student. "Next, we tilt the device and the cells slide into microscopic finger-like channels that are attached to the well. Finally, we return the device to its upright position, locking about 25 T cells inside each channel and essentially 'freezing' them in place."

The device has several advantages over current methods. First and foremost, individual cells stay in place so investigators can follow them over time with single-cell, time-lapse microscopy. Second, the fact that each T cell is suspended in nutrients in close physical contact with other cells results in near optimal conditions for keeping the infected cell alive for the virus' entire life cycle.

"This means that we now have the potential to analyze the entire course of an HIV infection in an individual cellespecially during the crucial latency stagefor which we know so little," said Dr. Weinberger. "In the future, we plan to expand the device's design to include a larger number of wells and channels to track HIV infection on a larger scale. We want to use the information gleaned here to finally unravel the mechanisms behind HIV latency. With that knowledge, we hope to devise a treatment to bring the latent virus out of hiding in order to flush it from a patient's system, once and for all."


'/>"/>
Contact: Anne Holden
anne.holden@gladstone.ucsf.edu
415-734-2534
Gladstone Institutes
Source:Eurekalert  

Related medicine news :

1. Gladstones Lennart Mucke receives Lifetime Achievement Award from the Alzheimers Association
2. Reconstructed 1918 influenza virus has yielded key insights, scientists say
3. Scientists put a pox on dog cancer
4. MBL and Stanford scientists receive 2012 Lasker Award for Basic Medical Research
5. Scientists Inch Closer to Genetic Blueprint of Diseases
6. Scientists Find Links Among Parkinsons, Cancer and Family History
7. Danish scientists solve old blood mystery
8. First validated method for analyzing flavanols and procyanidins in cocoa products could help scientists and the industry in standardized reporting
9. Scientists ID Happy Gene in Women
10. NIH scientists map first steps in flu antibody development
11. Scientists to design drug for chronic pain
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Gladstone scientists develop technique to decipher the dormant AIDS virus concealed in cells
(Date:2/22/2017)... , ... February 22, 2017 , ... ... and 72 percent of those report that family members or friends have also ... they suffer from hearing loss wear hearing aids. One reason, suggested by 89 ...
(Date:2/22/2017)... ... February 22, 2017 , ... Social media marketing is transitioning from a ... systems. Smith & Jones’ delves into this insight and more in its latest episode ... Jones David Vener meets up with social media strategist and partner of the digital ...
(Date:2/22/2017)... Kentucky (PRWEB) , ... February 22, 2017 , ... ... Journal of Patient Safety, patient advocates stress that the patient context (age, illness ... not used as reasons to mitigate their occurrence. In addition, all ...
(Date:2/21/2017)... Australia (PRWEB) , ... February 22, 2017 , ... Author ... it is valuable for every household and family to know all about it for ... publishing world with the release of “ Detox, Digestive and Wellness Solutions ” (published ...
(Date:2/21/2017)... , ... February 21, 2017 , ... Doctors on Liens ... directed by Dr. Kendell Mendonca , to its growing network of doctors in ... including injuries stemming from car accidents such as whiplash, back pain, neck pain, hip ...
Breaking Medicine News(10 mins):
(Date:2/22/2017)... , Feb. 22, 2017  Bioness, Inc., the leading ... announced the first series of successful StimRouter Neuromodulation System ... University Medical Center (Nijmegen, Netherlands ), ... Cork, Ireland ), and Kliniek Park Leopold Chirec ... launch continuing, Bioness plans to further support clinicians across ...
(Date:2/22/2017)... February 22, 2017 According to a new market ... In-Line, At-Line), Products and Services (Analyzers, Probes & Sensors), End - User ... MarketsandMarkets, the global market is poised to reach USD 3.30 Billion by ... from 2016 to 2021. Continue Reading ... ...
(Date:2/22/2017)... 22, 2017 Kineta, Inc., a biotechnology ... immune modulating therapies, today announced that KCP-400 (RgIA4), ... receptor (nAChR), demonstrates robust chronic pain relief and ... study also establishes the a9a10 nAChR as a ... pain. The findings were reported online in the ...
Breaking Medicine Technology: