Navigation Links
Genomic assay as an alternative to animal testing

The method developed by the group in Lund is based on human cells grown in a laboratory. The cells are exposed to a chemical and then parts of their genetic content are filtered out and transferred to a microchip.

"Using the chip, we can then read which genes reacted to the chemical and how they reacted. This provides a 'signature' of gene expression that shows whether the substance is allergenic", explains Malin Lindstedt.

The test has now been submitted for prevalidation to the EU agency that approves alternatives to animal testing. In the future, it could also be used in connection with the EU's chemicals legislation, REACH, which requires extensive testing of new chemicals. Under the rules in REACH, tens of thousands of chemicals will have to be tested, meaning many animal experiments to investigate the substances' potential allergy risk alone is estimated to require over 800 000 animals.

For allergy tests, guinea pigs and mice are the most commonly used animals. With guinea pigs, the test substance is injected under the skin to see if it produces redness, swelling or blisters. With mice, the substance is injected into the skin of the ear. The mice are put down a few days later so that the immunological reaction in their lymph nodes can be analysed.

Using animal experiments is ethically problematic. However, it also takes a lot of time and is therefore expensive. What is more, the results are not always reliable.

"The tests we have developed are more relevant for our health because they are based on human cells. Animal tests sometimes produce misleading results, which is only discovered afterwards once a substance has already caused allergic reactions", says Malin Lindstedt.

The Lund group started by developing a test for contact allergies. Subsequently, another test has been developed that can determine which substances could produce respiratory allergies, which often affect those who work in the chemicals industry.

"We are also developing the technology itself, to make it simpler and easier to manage. It will soon be possible to screen hundreds of chemicals at a time, which will be much, much quicker and cheaper", says Malin Lindstedt.


Contact: Malin Lindstedt
Lund University

Related medicine news :

1. Laboratory Automation Market: 2017 Equipment & Software, Drug Discovery, Clinical Diagnostics, Genomics & Proteomics Analysis in New Research Reports at RnRMarketResearch
2. Mayo Clinic genomic analysis lends insight to prostate cancer
3. Why We Cant Wait: Conference to Eliminate Health Disparities in Genomic Medicine
4. Large genomic study identifies endometrial cancer subtypes, treatment opportunities
5. Genomic detectives crack the case of the missing heritability
6. The science of uncertainty in genomic medicine
7. Deep genomic analysis identifies a micro RNA opponent for ovarian cancer
8. Nurses at forefront of genomics in health care
9. Genomic sequencing identifies mutant drivers of common brain tumor
10. Epigenomic abnormalities predict patient survival in non-Hodgkins lymphoma
11. When will genomic research translate into clinical care -- and at what cost?
Post Your Comments:
(Date:11/30/2015)... ... November 30, 2015 , ... Newly reviewed ... of Glen Ridge, NJ. He has both advanced training and considerable experience ... is also an expert in cosmetic dentistry. He is an active Spear ...
(Date:11/30/2015)... , ... November 30, 2015 , ... ... Poison Control Centers’ National Poison Data System (NPDS) reveals that in 2014, someone ... 3 million cases, over two million of which were human exposure cases. , ...
(Date:11/30/2015)... ... 2015 , ... The Foundation for Breast and Prostate Health ... forces with the award-winning creator and writer of Downton Abbey Julian Fellowes to ... the Union League of Philadelphia. , The benefit, titled “An Evening with ...
(Date:11/30/2015)... ... November 30, 2015 , ... According to RF Safe, ... senior scientist at Consumer Reports as supporting a “A Call for Clarity” on ... , The original Nov 2015 CR story titled, “Does Cell-Phone Radiation Cause Cancer?” ...
(Date:11/30/2015)... ... November 30, 2015 , ... An inventor from Charlottesville, Va., ... womb. "My last baby had high blood pressure due to loud noises," she said, ... their babies from noise pollution as well as radio waves and microwaves." , The ...
Breaking Medicine News(10 mins):
(Date:11/30/2015)... , Nov. 30, 2015   Nuance Communications, Inc. ... National Decision Support Company (NDSC) today jointly announced ... collaboration capabilities that utilize the American College of Radiology,s ... provider organizations to comply with current and emerging ... --> By combining clinical decision support, ...
(Date:11/30/2015)... , Nov. 30, 2015  Novartis will demonstrate the ... 57 th American Society of Hematology (ASH) Annual ... myelomas as well as supportive care, including key findings ... personalized cell therapies. The ASH Annual Meeting will be ... . Novartis Oncology . "We will be ...
(Date:11/30/2015)... Israel , Nov. 30, 2015  OticPharma, Ltd., ... and developing innovative therapies for ear, nose, and throat ... A. Katkin as chairman of the company,s board ... executive officer for OticPharma, Ltd.  "Keith brings a wealth ... will be able to share this experience and provide ...
Breaking Medicine Technology: