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Genome sequencing used to assess a novel form of Clostridium botulinum
Date:3/7/2011

C on our doorstep was extremely useful for this project, and will be a boon for IFR science," said Professor Peck. "It has enabled us to evaluate the threat that this new strain may pose to food safety and biosecurity by rapidly delivering a complete genome, finished genome sequence."

IFR and TGAC are institutes of the Biotechnology and Biological Sciences Research Council (BBSRC), which funded this research.

The genome was annotated at TGAC by Dr Lisa Crossman. Annotating the genome involves looking at the genomic sequence, and working out where the genes themselves are, and what they may do. This is done by comparison with genes of known function from other bacteria.

"We first annotated the genome automatically, but because of the importance in obtaining a high quality set of genes, we manually curated the most interesting parts of the genome," said Dr Crossman.

The 3.9 Mb genome is structurally similar to other strains of C. botulinum. The scientists identified the gene that produces the neurotoxin, which is in the same location as in many other C. botulinum strains, and confirmed that no other neurotoxin genes were present. The genes needed for spore germination are similar to those in related strains.


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Contact: Andrew Chapple
andrew.chapple@bbsrc.ac.uk
44-016-032-51490
Norwich BioScience Institutes
Source:Eurekalert

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