Navigation Links
Genome sequencing used to assess a novel form of Clostridium botulinum
Date:3/7/2011

Scientists on the Norwich Research Park have sequenced the genome of a novel strain of Clostridium botulinum, one of the most dangerous pathogens known to man. The strain produces an unusual botulinum neurotoxin, known as type A5 neurotoxin, which was isolated by the Health Protection Agency (HPA), following a case of wound botulism.

Professor Mike Peck and his research group at the Institute of Food Research (IFR) study Clostridium botulinum. Their expertise is crucial for preventing food poisoning outbreaks in the UK and internationally and to understanding the threat that these neurotoxin-producing bacteria pose to biosecurity. Outbreaks of foodborne botulism are extremely rare, due to the diligent way in which research scientists and the food industry collaborate to minimise the risks.

"Our analysis of the genome sequence indicates that while the type of neurotoxin formed is unusual, the organism itself appears closely related to other strains of Clostridium botulinum, and is likely to pose a similar threat to food safety and biosecurity" said Professor Peck.

The novel A5 neurotoxin and its gene sequence were first discovered by Professor Peck's research team, and other laboratories are now analysing this specific neurotoxin and assessing the implications for food safety and biosecurity.

Having the complete genome sequence is crucial to managing the potential threat that the new strain poses. The complete genome sequence of the strain was generated by The Genome Analysis Centre (TGAC), and will help in the study of Clostridium botulinum and botulism in general.

High throughput sequencing was performed at TGAC using the Roche 454 and Illumina GAII next generation sequencing platforms with the longer 454 reads assembled first and followed by Illumina reads to correct ambiguities. Remaining gaps in the assembly were closed by Prof Peck's group at IFR using other sequencing methods.

"Having TGAC on our doorstep was extremely useful for this project, and will be a boon for IFR science," said Professor Peck. "It has enabled us to evaluate the threat that this new strain may pose to food safety and biosecurity by rapidly delivering a complete genome, finished genome sequence."

IFR and TGAC are institutes of the Biotechnology and Biological Sciences Research Council (BBSRC), which funded this research.

The genome was annotated at TGAC by Dr Lisa Crossman. Annotating the genome involves looking at the genomic sequence, and working out where the genes themselves are, and what they may do. This is done by comparison with genes of known function from other bacteria.

"We first annotated the genome automatically, but because of the importance in obtaining a high quality set of genes, we manually curated the most interesting parts of the genome," said Dr Crossman.

The 3.9 Mb genome is structurally similar to other strains of C. botulinum. The scientists identified the gene that produces the neurotoxin, which is in the same location as in many other C. botulinum strains, and confirmed that no other neurotoxin genes were present. The genes needed for spore germination are similar to those in related strains.


'/>"/>

Contact: Andrew Chapple
andrew.chapple@bbsrc.ac.uk
44-016-032-51490
Norwich BioScience Institutes
Source:Eurekalert

Related medicine news :

1. WSU study may lead to greater understanding of human genome regulation
2. IDIBELL researchers discover a substance against the dark genome of cancer
3. Whole genome sequencing used to help inform cancer therapy
4. First Look at Prostate Cancer Genome Yields Insights
5. Orangutan Genomes Reveal Some Surprises
6. Punctuated evolution in cancer genomes
7. Genome code cracked for most common form of pediatric brain cancer
8. Targeted therapy reactivates guardian of the genome in resistant cancer
9. Wistar researchers discover new class of objects encoded within the genome
10. Genome Studies Point to Cholesterol-Regulating Genes
11. Of lice and man: Researchers sequence human body louse genome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... ... , ... Pixel Film Studios Released ProSlice Levels, a Media Slicing Effect ... a whole new perspective by using the title layers in ProSlice Levels to ... ProSlice Levels contains over 30 Different presets to choose from. FCPX users ...
(Date:6/25/2016)... D.C. (PRWEB) , ... June 25, 2016 , ... ... discuss health policy issues and applications at AcademyHealth’s Annual Research Meeting June 26-28, ... their work on several important health care topics including advance care planning, healthcare ...
(Date:6/25/2016)... ... June 25, 2016 , ... First Choice Emergency Room , the ... Ogunleye, as the Medical Director of its new Mesquite-Samuell Farm facility. , “We ... new Mesquite location,” said Dr. James M. Muzzarelli, Executive Medical Director of First Choice ...
(Date:6/25/2016)... Montreal, Canada (PRWEB) , ... June 25, 2016 , ... ... the pursuit of success. In terms of the latter, setting the bar too high ... low, risk more than just slow progress toward their goal. , Research from ...
(Date:6/24/2016)... ... June 24, 2016 , ... Marcy was in a crisis. Her son James, eight, was out ... family verbally and physically. , “When something upset him, he couldn’t control his emotions,” remembers ... would throw rocks at my other children and say he was going to kill them. ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... June 23, 2016 Any dentist who has made ... the current process. Many of them do not even offer ... difficulties and high laboratory costs involved. And those who ARE ... at such a high cost that the majority of today,s ... Dr. Parsa Zadeh , founder of Dental Evolutions Inc. ...
(Date:6/23/2016)... Research and Markets has announced the addition of the ... to 2022" report to their offering. ... financial data derived from varied research sources to present unique ... on the market during the next five years, including a ... markets, regional and country level analysis. The report provides a ...
(Date:6/23/2016)... MOUNTAIN VIEW, Calif. , June 23, 2016 ... 9:00 a.m. CST on Thursday, July 7, 2016 , , ... kayla.belcher@frost.com ) , , , , EXPERT PANELISTS:  ... Sciences, Nitin Naik; Senior Industry Analyst, Christi Bird; Senior Industry Analyst, ... The global pharmaceutical industry is witnessing ...
Breaking Medicine Technology: