Navigation Links
Genome sequencing used to assess a novel form of Clostridium botulinum
Date:3/7/2011

Scientists on the Norwich Research Park have sequenced the genome of a novel strain of Clostridium botulinum, one of the most dangerous pathogens known to man. The strain produces an unusual botulinum neurotoxin, known as type A5 neurotoxin, which was isolated by the Health Protection Agency (HPA), following a case of wound botulism.

Professor Mike Peck and his research group at the Institute of Food Research (IFR) study Clostridium botulinum. Their expertise is crucial for preventing food poisoning outbreaks in the UK and internationally and to understanding the threat that these neurotoxin-producing bacteria pose to biosecurity. Outbreaks of foodborne botulism are extremely rare, due to the diligent way in which research scientists and the food industry collaborate to minimise the risks.

"Our analysis of the genome sequence indicates that while the type of neurotoxin formed is unusual, the organism itself appears closely related to other strains of Clostridium botulinum, and is likely to pose a similar threat to food safety and biosecurity" said Professor Peck.

The novel A5 neurotoxin and its gene sequence were first discovered by Professor Peck's research team, and other laboratories are now analysing this specific neurotoxin and assessing the implications for food safety and biosecurity.

Having the complete genome sequence is crucial to managing the potential threat that the new strain poses. The complete genome sequence of the strain was generated by The Genome Analysis Centre (TGAC), and will help in the study of Clostridium botulinum and botulism in general.

High throughput sequencing was performed at TGAC using the Roche 454 and Illumina GAII next generation sequencing platforms with the longer 454 reads assembled first and followed by Illumina reads to correct ambiguities. Remaining gaps in the assembly were closed by Prof Peck's group at IFR using other sequencing methods.

"Having TGAC on our doorstep was extremely useful for this project, and will be a boon for IFR science," said Professor Peck. "It has enabled us to evaluate the threat that this new strain may pose to food safety and biosecurity by rapidly delivering a complete genome, finished genome sequence."

IFR and TGAC are institutes of the Biotechnology and Biological Sciences Research Council (BBSRC), which funded this research.

The genome was annotated at TGAC by Dr Lisa Crossman. Annotating the genome involves looking at the genomic sequence, and working out where the genes themselves are, and what they may do. This is done by comparison with genes of known function from other bacteria.

"We first annotated the genome automatically, but because of the importance in obtaining a high quality set of genes, we manually curated the most interesting parts of the genome," said Dr Crossman.

The 3.9 Mb genome is structurally similar to other strains of C. botulinum. The scientists identified the gene that produces the neurotoxin, which is in the same location as in many other C. botulinum strains, and confirmed that no other neurotoxin genes were present. The genes needed for spore germination are similar to those in related strains.


'/>"/>

Contact: Andrew Chapple
andrew.chapple@bbsrc.ac.uk
44-016-032-51490
Norwich BioScience Institutes
Source:Eurekalert

Related medicine news :

1. WSU study may lead to greater understanding of human genome regulation
2. IDIBELL researchers discover a substance against the dark genome of cancer
3. Whole genome sequencing used to help inform cancer therapy
4. First Look at Prostate Cancer Genome Yields Insights
5. Orangutan Genomes Reveal Some Surprises
6. Punctuated evolution in cancer genomes
7. Genome code cracked for most common form of pediatric brain cancer
8. Targeted therapy reactivates guardian of the genome in resistant cancer
9. Wistar researchers discover new class of objects encoded within the genome
10. Genome Studies Point to Cholesterol-Regulating Genes
11. Of lice and man: Researchers sequence human body louse genome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/9/2016)... ... December 09, 2016 , ... Sober College, ... with the grand opening of the Sober College Robert Pfeifer Memorial Learning Center ... December 2-3, and was attended by an overwhelming amount of alumni, family, colleagues ...
(Date:12/9/2016)... , ... December 09, 2016 , ... "I had a ... inventor from Winchester, Va. "I thought that if the nebulizer had a more child-friendly ... than fearing them." , He developed the patent-pending NEBY to avoid the need to ...
(Date:12/8/2016)... , ... December 08, 2016 , ... ... Franchising Company LLC, announced the first national #QuackGivesBack campaign which supported local ... “This was our first franchise-wide Quack Gives Back initiative, and we’re ...
(Date:12/8/2016)... ... December 08, 2016 , ... With the increasing demand for dental implants, the ... (WIYM) campaign to inform dentists and patients about the safety issues related to dental ... market in the U.S. is projected to reach $6.4 billion in 2018 with more ...
(Date:12/8/2016)... ... 2016 , ... Catalent Pharma Solutions, the leading global provider ... products, today announced that it had joined the Pharmaceutical Supply Chain Initiative (PSCI). ... unite pharmaceutical and healthcare companies that share a vision of better, social, environmental ...
Breaking Medicine News(10 mins):
(Date:12/8/2016)... , Dec. 8, 2016  Eli Lilly and ... results of its phase 3 EXPEDITION3 trial at the ... meeting. As previously disclosed, solanezumab did not meet the ... of solanezumab initiated in people with mild dementia due ... regulatory submissions for solanezumab for the treatment of mild ...
(Date:12/8/2016)... de dezembro de 2016  A Mederi Therapeutics Inc . anunciou aprovação ... não cirúrgico para a doença do refluxo gastroesofágico (DRGE). Foto -  ... ... Live Stretta procedure performed and broadcast during the Chinese ... Union Hospital , ...
(Date:12/8/2016)... Pa. , Dec. 8, 2016  Pennsylvania ... Secretary of Drug and Alcohol Programs Gary ... and insomnia medications, known as benzodiazepines, developed with ... "Benzodiazepines are medications that are frequently prescribed ... when they are used with opioid pain medications, ...
Breaking Medicine Technology: