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GEN reports on advances in DNA vaccine delivery and production
Date:9/1/2011

New Rochelle, NY, September 1, 2011Scientists involved in DNA vaccine research are currently focused on two major issues: the creation of effective delivery systems and the development of more efficient biomanufacturing strategies, reports Genetic Engineering & Biotechnology News (GEN). Top investigators in the field recently discussed these and other topics at a conference in San Diego entitled "DNA Vaccines: Building on Clinical Progress and Exploring New Targets," which was sponsored by the International Society of DNA Vaccines and organized by BioConferences International, a Mary Ann Liebert company, according to the September 1 issue of GEN. http://www.genengnews.com/issue/toc/0/

David Weiner, Ph.D., University of Pennsylvania professor and conference chair, noted that despite some early setbacks, DNA vaccine technology has advanced dramatically over the past few years and that therapies currently under evaluation are demonstrating the superb potential of plasmid-based vaccines. He emphasized that a range of novel vaccine methodologies have emerged, including new strategies for getting the plasmids into cells, increasing protein production once they are inside, and modifications of the vaccine proteins that increase their recognition and response by the immune system.

Some of the more promising delivery approaches include transdermal, needle-free patches, devices that transport the plasmids into the skin via air pressure, and electroporation, in which electrical pulses are used to temporarily open the cell membrane, allowing the plasmids easier access to the interior of cells.

On the manufacturing front, Philippe Ledent, Ph.D., process transfer and development manager at Eurogentec Biologics, explained that his company has faced major challenges scaling up the output of both protein and nucleic acid products. For plasmid production Eurogentec turned to fed-batch protocols for
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Contact: John Sterling
jsterling@genengnews.com
914-740-2196
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert

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