Navigation Links
Fruit fly phlebotomy holds neuroscience promise
Date:3/25/2008

Drawing blood from a fruit fly may only be slightly easier than getting it from a proverbial stone or turnip, but success could provide substantial benefits for neuroscientists.

Researchers at the University of Illinois at Chicago managed the feat and say their method could expedite understanding of the physiology of important insects such as Drosophila melanogaster, the common laboratory fruit fly that shares almost three-quarters of its genetic code with humans.

Scott Shippy, associate professor of chemistry, and doctoral student Sujeewa Piyankarage developed the technique while assisting UIC neuroscientist David Featherstone, who wanted to analyze the blood from two genetic types of fruit flies he was studying.

Under a microscope, the researchers managed to scrape an incision along the body of a fruit fly larva causing it to leak hemolymph -- insect blood -- onto the underlying collecting plate, and then vacuum it up through a narrow tube, getting enough sample for analysis.

The technique enabled them to gather from 50 to 300 nanoliters -- billionths of a liter -- of fluid, about one-thousandth of a drop, without significant evaporation, even when performed in open-air conditions that are prone to evaporation.

Traditional methods require that several flies or larvae be homogenized to obtain a large enough sample for analysis. In the new method, only a single larva is used, and only one biological fluid -- the hemolymph -- is extracted.

"We know we have hemolymph and nothing else," said Shippy. "It's not diluted with any other cells. And we're doing it on an individual organism."

The method opens up the possibility to study an individual, rather than a general population, to learn how body chemistry affects neurological function.

Fruit flies serve as particularly good laboratory animals because of their ability to quickly breed new generations, including ones with genetic mutations that are analogues to genes that cause human diseases.

"They're exceedingly powerful genetic tools," said Shippy.

He said the method could also be used to extract biological fluids from adult flies, as well as from other important laboratory insects, such as cockroaches, where tiny amounts of fluid could be analyzed to study the workings of neural circuitry.

Shippy said the method might also be used for extracting fluid from humans to pinpoint where diseases are just starting.

"We're particularly interested in retinal diseases," he said. "Disease doesn't happen across the whole of the retina, in many cases. Often there are small hot-spots where a disease might start. It would be very interesting to have a tool, or means to collect small volumes from areas where there's a problem, where there's not a problem, and places in between, to follow what's happening."


'/>"/>

Contact: Paul Francuch
francuch@uic.edu
312-996-3457
University of Illinois at Chicago
Source:Eurekalert

Related medicine news :

1. Tahitian Noni International Expands HIRO(TM) Beverage Line With Super Fruits Sparkling Black Currant
2. Grapefruit compound may help combat hepatitis C infection
3. Consumption of fruits may reduce the risk of Alzheimers disease
4. Natural Nutrition Launches All Natural FUSION Fruit Bar Line
5. Fruit flies all aglow light the way to cancer prevention
6. School Salad Bar Boosts Kids Fruit, Veggie Intake
7. For the fruit fly, everything changes after sex
8. Green Tea, Fruit Extracts Touted as Potential Cancer Fighters
9. Diet and cancer prevention: New evidence for the protective effects of fruits and veggies
10. Why Fruit Gets Short Shrift in U.S. Diet: Online Poll
11. Want to Raise a Fruit-and-Veggie Lover? Be Persistent
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/22/2017)... ... February 22, 2017 , ... Using the power of the ... simple online checklist. Over a period of just 24 months, thousands of individuals interested ... online checklist called T.A.D. , “The internet is not getting quieter. In fact it’s ...
(Date:2/22/2017)... (PRWEB) , ... February 22, 2017 , ... ANGLESTRONG ... Kurt Angle, is now available on the App Store and Google ... mobile apps for the addiction and recovery industry, partnered with Angle to build ANGLESTRONG. ...
(Date:2/22/2017)... ... February 22, 2017 , ... Becker’s Spine Review, ... is featuring Michigan neurosurgeon Jay Jagannathan, M.D., as a “Spine Surgeon to Know.” ... of neurosurgeons in Michigan performing minimally invasive back surgery that often results in ...
(Date:2/22/2017)... , ... February 22, 2017 , ... A product of ... seem to derive a heart-protective benefit from eating soy foods, while others do not, ... men who are able to produce equol—a substance made by some types of “good” ...
(Date:2/22/2017)... ... 2017 , ... Forty-five percent of American respondents feel that ... or friends have also commented about their poor hearing. However, only 13 percent ... reason, suggested by 89 percent of American respondents, is that they don’t want ...
Breaking Medicine News(10 mins):
(Date:2/22/2017)... Summary Provides understanding and ... entered into by the worlds leading healthcare companies. ... Description The Global Atherosclerosis Partnering Terms and ... to partnering deals and agreements entered into by ... Trends in partnering deals - Top deals ...
(Date:2/22/2017)... Summary Provides understanding and access ... entered into by the worlds leading healthcare companies. ... The Global Motor Neurone Disease Partnering Terms and Agreements ... deals and agreements entered into by the world,s leading ... - Top deals by value - Deals ...
(Date:2/22/2017)... 2017 The top three ... Olympus Corporation, Nikon Corporation, and Leica Microsystems. Collectively ... the overall market in 2015. The oligopolistic market ... result-oriented research and development activities and mergers and ... on expanding their business to emerging economies as ...
Breaking Medicine Technology: