Navigation Links
Fruit fly phlebotomy holds neuroscience promise
Date:3/25/2008

Drawing blood from a fruit fly may only be slightly easier than getting it from a proverbial stone or turnip, but success could provide substantial benefits for neuroscientists.

Researchers at the University of Illinois at Chicago managed the feat and say their method could expedite understanding of the physiology of important insects such as Drosophila melanogaster, the common laboratory fruit fly that shares almost three-quarters of its genetic code with humans.

Scott Shippy, associate professor of chemistry, and doctoral student Sujeewa Piyankarage developed the technique while assisting UIC neuroscientist David Featherstone, who wanted to analyze the blood from two genetic types of fruit flies he was studying.

Under a microscope, the researchers managed to scrape an incision along the body of a fruit fly larva causing it to leak hemolymph -- insect blood -- onto the underlying collecting plate, and then vacuum it up through a narrow tube, getting enough sample for analysis.

The technique enabled them to gather from 50 to 300 nanoliters -- billionths of a liter -- of fluid, about one-thousandth of a drop, without significant evaporation, even when performed in open-air conditions that are prone to evaporation.

Traditional methods require that several flies or larvae be homogenized to obtain a large enough sample for analysis. In the new method, only a single larva is used, and only one biological fluid -- the hemolymph -- is extracted.

"We know we have hemolymph and nothing else," said Shippy. "It's not diluted with any other cells. And we're doing it on an individual organism."

The method opens up the possibility to study an individual, rather than a general population, to learn how body chemistry affects neurological function.

Fruit flies serve as particularly good laboratory animals because of their ability to quickly breed new generations, including ones with genetic mutations that are analogues to genes that cause human diseases.

"They're exceedingly powerful genetic tools," said Shippy.

He said the method could also be used to extract biological fluids from adult flies, as well as from other important laboratory insects, such as cockroaches, where tiny amounts of fluid could be analyzed to study the workings of neural circuitry.

Shippy said the method might also be used for extracting fluid from humans to pinpoint where diseases are just starting.

"We're particularly interested in retinal diseases," he said. "Disease doesn't happen across the whole of the retina, in many cases. Often there are small hot-spots where a disease might start. It would be very interesting to have a tool, or means to collect small volumes from areas where there's a problem, where there's not a problem, and places in between, to follow what's happening."


'/>"/>

Contact: Paul Francuch
francuch@uic.edu
312-996-3457
University of Illinois at Chicago
Source:Eurekalert

Related medicine news :

1. Tahitian Noni International Expands HIRO(TM) Beverage Line With Super Fruits Sparkling Black Currant
2. Grapefruit compound may help combat hepatitis C infection
3. Consumption of fruits may reduce the risk of Alzheimers disease
4. Natural Nutrition Launches All Natural FUSION Fruit Bar Line
5. Fruit flies all aglow light the way to cancer prevention
6. School Salad Bar Boosts Kids Fruit, Veggie Intake
7. For the fruit fly, everything changes after sex
8. Green Tea, Fruit Extracts Touted as Potential Cancer Fighters
9. Diet and cancer prevention: New evidence for the protective effects of fruits and veggies
10. Why Fruit Gets Short Shrift in U.S. Diet: Online Poll
11. Want to Raise a Fruit-and-Veggie Lover? Be Persistent
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/18/2017)... ... ... Overseer at The House of Yahweh in Abilene, Texas, has released a new publication this ... of the Savior whom the world calls “Mother Mary”. Yisrayl says when compared to what ... historical woman. , “The world bows, kisses the feet of, cries out to and ...
(Date:8/18/2017)... ... August 18, 2017 , ... 800response, the leading ... solutions, announced today the launch of a redesigned corporate website, 800response.com . ... fully responsive design, and an enhanced search directory for businesses and advertising agencies ...
(Date:8/18/2017)... ... ... “Three Narrow Roads”: a vivid and provocative look into the narrow path toward ... Sr., is currently the pastor-teacher-visionary at Simply the WORD Church, a global prayer ministry ... program geared towards youth. , King shares, “When I take time to think about ...
(Date:8/18/2017)... (PRWEB) , ... August 18, 2017 , ... ... and marketers of high-quality anti-aging skincare solutions, recently announced the launch ... new products are an affordable, yet effective alternative to expensive plastic surgery or ...
(Date:8/18/2017)... ... ... “Steve the Snake”: an entertaining and moral-based short story about an unkind snake ... Flash Haskins Jr., a husband, father and grandfather who served as a U.S. Marine, ... short stories for children and teens. My goal is to teach kids to use ...
Breaking Medicine News(10 mins):
(Date:7/31/2017)... July 31, 2017 7D Surgical, developer of ground ... purchased the 7D Surgical System to support its strategic sales ... D.C. and Virginia.  7D Surgical has entered into ... of the premier medical facilities within those markets. ... ...
(Date:7/27/2017)... Holdings, Inc. (NYSE and SIX: ZBH) today reported financial results ... reported second quarter net sales of $1.954 billion, an ... an increase of 2.1% on a constant currency basis.  ... LDR Holding Corporation acquisition, second quarter 2017 revenues decreased ... on a constant currency basis. ...
(Date:7/26/2017)... a leading medical aesthetic clinic in Singapore ... conditions from the inside. The natural process of aging ... becomes more transparent due to the thinning of the epidermis. Over the ... sun contributes to aging skin, causing age spots and other types ... ...
Breaking Medicine Technology: