Navigation Links
Following a protein's travel inside cells is key to improving patient monitoring, drug development
Date:4/23/2014

In science, "simple and accessible detection methods that can rapidly screen a large cell population with the resolution of a single cell inside that population has been seriously lacking," said Virginia Tech chemical engineer Chang Lu.

In the Royal Society of Chemistry journal Chemical Science, Lu has announced that he and his coworkers have developed a novel technique that detects the subcellular location of a protein (Chem. Sci., 2014, DOI: 10.1039/C4SC00578C, http://pubs.rsc.org/en/content/articlelanding/2014/sc/c4sc00578c#!divAbstract). The significance of this method is that the technique will allow the scientific and technological communities a simple and improved method for studying effectiveness of therapies for disease, including cancer.

If a protein is not located in the right subcellular compartment of a cell, "the result can be diseases ranging from metabolic disorders to cancers," Lu explained. "Modulation of protein transport inside a cell is practiced as an important therapeutic approach for cancer treatment. The subcellular location of a target protein can also serve as a useful read-out for high-content screening of cancer drugs."

In the human body, proteins move between distinct compartments inside cells, including the plasma membrane, the nucleus, and other membrane-enclosed areas. This movement can be a prerequisite for proteins to carry out their intended functions. These functions might include gene transcription and other molecular regulations.

In the publication, Lu and his colleagues, Zhenning Cao, a graduate student of the School of Biomedical Engineering and Sciences, Shuo Geng, a postdoctoral researcher, and Liwu Li, professor of biological sciences, all of Virginia Tech, described the shortcomings of current evaluations of protein movement.

One such technique is fluorescence microscopy. According to Lu, it can only analyze a limited number of cells. Data collected by a second existing assay called subcellular fractionation only reflects the average properties of the cell populations "without revealing the heterogeneity that is often present among seemingly identical cells," they stated.

Lu' team had made some progress in screening cell populations in the past using an electroporation-based technique, but it did not allow the examination of native proteins and primary cells isolated from animals and from patients.

Their new work uses a method that "incorporates selective chemical release of cytosolic proteins with a standard procedure for fluorescent labeling of the protein to detect the subcellular location of a native protein," Lu said. This simple and unique tweak to the conventional cell staining process allowed them to accurately define the subcellular location of the protein by measuring the amount of the residual protein after release. Using a flow cytometer, the speed of such measurement could reach 10,000 to 100,000 cells per second.

A key ingredient to their process is the use of saponin, a class of amphipathic glycosides. It dissolves cholesterol and permeates the plasma membrane to allow protein release. "Gentle treatment by saponin shows minimal effects on the state of the cell," Lu added.


'/>"/>
Contact: Lynn Nystrom
tansy@vt.edu
540-231-4371
Virginia Tech
Source:Eurekalert  

Related medicine news :

1. Scientists discover a new way to enhance nerve growth following injury
2. Medication does not help prevent ED following radiation therapy for prostate cancer
3. Patients with metabolic disorder may face higher complication risk following total joint replacement
4. Major League Baseball players win more games following Tommy John surgery
5. Is therapeutic hypothermia beneficial in all patients following cardiac arrest?
6. High potency statins linked to better outcome following a heart attack
7. Allsup Explains Why Social Security Disability Claims Can Be Denied Following Recent Report
8. Insight into likelihood of retinal detachment following open globe injury
9. RI researchers validate tool for pain assessment in patients following cardiac surgery
10. Kids II® Congratulates Singer Gwen Stefani Following Announcement of her Third Pregnancy, Offers Baby Gift Ideas
11. Parkinsons disease patients following subthalamic nucleus deep brain stimulation: fully understanding of social maladjustment
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Following a protein's travel inside cells is key to improving patient monitoring, drug development
(Date:10/13/2017)... (PRWEB) , ... October 13, ... ... School of Pharmacy (SOP) alumni Hannah Randall, PharmD ‘17, and Jennifer Huggins, ... professionals on guideline updates for the primary prevention of cardiovascular diseases during ...
(Date:10/13/2017)... ... October 13, 2017 , ... Apple Rehab Shelton Lakes ... a mock evacuation of the facility as part of a disaster drill on October ... Hose EMS and Shelton City Emergency Manager, as well as the Connecticut Long ...
(Date:10/13/2017)... , ... October 13, 2017 , ... The Visiting Nurse ... Market. Featuring a collection of specialty vendors and unique items from across the nation, ... quality-focused health and wellness services offered by the VNA. The boutique will be ...
(Date:10/13/2017)... NJ (PRWEB) , ... October 13, 2017 , ... Global ... at scenic Alexandria Park in Milford, NJ. This free event, sponsored by Global ... physical activity. The fun run is geared towards children of all ages; it ...
(Date:10/13/2017)... ... October 13, 2017 , ... Talented host, actor Rob ... sciatica in a new episode of "Success Files," which is an award-winning educational ... and investigates each subject in-depth with passion and integrity. , Sciatica occurs when ...
Breaking Medicine News(10 mins):
(Date:10/11/2017)... PARK, Calif. , Oct. 11, 2017  BioPharmX ... national scientific team that developed an innovative way to ... quantity of the delivery of new drugs. ... 2017 Fall Clinical Dermatology Conference will show how researchers ... General Hospital, Harvard Medical School used a suite of ...
(Date:10/10/2017)... 10, 2017  NDS received FDA 510(k) clearance in May 2017 ... display stand specifically designed for endoscopy environments. An innovative secondary monitor ... clinical solution to support the improvement of patient outcomes, procedural efficiency, ... ... ...
(Date:10/4/2017)... Korea , Oct. 4, 2017  South Korean-based ... next-generation CPR training aide "cprCUBE" on Kickstarter. The device ... compression during cardiac arrests with better efficiency compared to ... also offers real-time feedback on efficacy of the compression ... crowdfunding campaign has a goal to raise $5,000. ...
Breaking Medicine Technology: