Navigation Links
Fluorescent probes may permit monitoring of chemotherapy effectiveness, Stanford study shows
Date:7/13/2009

things," Salvesen said. "First, they've got to learn to start dividing rapidly. But once they do that, they become very vulnerable to apoptosis. So they've got to learn to switch off this death mechanism. Chemotherapy and radiotherapy aim to turn it back on."

One way of determining whether they're succeeded is by monitoring key early players in apoptosis called caspases, a family of usually quiescent enzymes found inside every mammalian cell. Activated by various biochemical cues from within or outside of the cell, caspases commence a cascade of molecular steps that steer the cell to a clean, quiet, orderly death.

"Caspases have to be very tightly controlled, since they are regulating cell death. If they get turned on, the cell dies," said Bogyo. His team created probes by affixing fluorescent "tags" to small molecules that were engineered to bind and stay bound almost exclusively to caspases, and only when the caspases are in an active state. The resulting probes are excited by certain wavelengths of light that travel through skin without being absorbed. The probes respond by giving off their own light, which can be imaged by a special detector.

"Our probe can't bind to inactive caspases," Bogyo said. "It can go into cells, but it doesn't get stuck it just circulates back out. So the only cells that fluoresce are the ones approaching death."

In an early test of the probe, Bogyo and his colleagues gave mice a drug called dexamethosone, which preferentially induces apoptosis in certain immature immune cells residing primarily in the thymus. After systemically injecting a solution containing the probes, the investigators observed fluorescence in the thymus, as predicted. They confirmed by chemical methods that the fluorescent probes were indeed binding to caspases.

Next, the team performed experiments with a new, experimental monoclonal antibody that activates caspases, by a mechanism different from that of dexamethasone
'/>"/>

Contact: Bruce Goldman
goldmanb@stanford.edu
650-725-2106
Stanford University Medical Center
Source:Eurekalert

Page: 1 2 3

Related medicine news :

1. Naturally fluorescent molecules may serve as cancer biomarker
2. MBL scientist Osamu Shimomura wins Nobel Prize for discovery of green fluorescent protein
3. Fluorescent Retinal Cells Warn of Eye Disease
4. MEDRAD Multi Vendor Service Launches TotalRepair(SM) on Select Ultrasound Probes
5. Nanoscopic probes can track down and attack cancer cells
6. China Medical Technologies Announces the Receipt of SFDA Approval for Two of its FISH Probes and the Approval for the Hi-Tech Enterprise Status for its PRC Subsidiaries
7. Team probes why climbers die on Mount Everest
8. Study Probes Why Smokers Find It Hard to Quit
9. Study Probes Internet Health-Related Search Patterns
10. String probes for devastating childhood digestive disease
11. Matrix registry probes adherence to clopidogrel at 1 year
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/21/2014)... Faculty and staff at Garrison Forest School ... Owings Mills, Md., kicked off the academic year by taking ... is the first independent school to receive the bands— which ... sleep patterns— as part of a pilot program with ... specialty chain. The Vivofit bands are just one tool in ...
(Date:9/21/2014)... (PRWEB) September 21, 2014 According to latest ... the highest birth rate since 1966 (10.5 births per 1,000 ... their early 40s has risen steadily by 2 percent per ... births to women aged 50 and over) also rose, the ... there were more than nine times as many first births ...
(Date:9/20/2014)... iFitDress.com, a well-known wedding dress manufacturer and retailer, has unveiled ... addition, the company has launched a special offer on the ... benefit all of its big fans. Worldwide people can buy ... Along with beautiful party dresses, the company also offers many ... new sexy short prom outfits and purple homecoming gowns online; ...
(Date:9/20/2014)... 2014 Diabetes Protocol , a ... is helping thousands of people learn how to naturally ... pills, or prescriptions has caught the attention of Shane ... currently tens of millions of people in the U.S. ... 2 diabetes, and unfortunately, big pharma makes a killing ...
(Date:9/20/2014)... 6 Minutes to Skinny , a fat loss ... is showing men and women of all ages how they ... using simple 6-minute exercises and easy-to-follow lifestyle tips daily, has ... , “When it comes to losing body fat, unfortunately ... all people,” reports Michaels. “Metabolism plays a big role in ...
Breaking Medicine News(10 mins):Health News:Garrison Forest School Leader in Independent School Wellness Programs 2Health News:Statistics Reveal That the Number of 'Older' First-Time Mothers Is Growing 2Health News:Statistics Reveal That the Number of 'Older' First-Time Mothers Is Growing 3Health News:Statistics Reveal That the Number of 'Older' First-Time Mothers Is Growing 4Health News:Beautiful Party Dresses For Autumn Unveiled By iFitDress.com 2Health News:Diabetes Protocol: Review Exposes Kenneth Pullman’s Breakthrough Discovery About Diabetes Management 2Health News:6 Minutes to Skinny: Review Exposes Craig Ballantyne’s Method to Jump Starting the Metabolism and Blasting Away Body Fat in Just 6 Minutes Per Day 2
... Konica Minolta Sensing Americas,Inc. (KMSA), the ... color,light and shape, announces the launch of ... product used in combination with Konica Minolta,s,portable ... simultaneously with a numerical display of the ...
... By playing it safe and using a two-pronged attack, a ... tested by an international team of researchers led by the ... similar to components of viruses and in this way alerts ... against cancer cells in this process. At the same time, ...
... Nurses Association to Ask Voters ... to Think of Health Care, TRENTON, N.J., Nov. 1 ... Ladies have,joined forces to ask the state,s public policy makers to ... and federal level. The First Ladies,joined the newest members of the ...
... , , SATURDAY, Nov. 1 (HealthDay News) -- Personalized treatment ... a UCLA study of almost 1,500 patients that identified ... to be treated accordingly. , The UCLA team said ... to be changed. , "We have shown that not ...
... the #1 position among detox procedures for opiate addiction, there is a ... , ... Palm Beach, FL (PRWEB) November 1, 2008 -- The primary ... as Meditox of Palm Beach that offer it? The drug can ...
... of nearly 1,500 patients treated for kidney cancer at ... aggressive, tailored treatment approach results in better survival rates ... and need to be treated accordingly. , The one-size-fits-all ... changed based on the results of the study, the ...
Cached Medicine News:Health News:Konica Minolta Sensing Americas Announces the Launch of the CM-SA Skin Analysis Software 2Health News:Substance tackles skin cancer from 2 sides 2Health News:On Weekend Before the Election, New Jersey's First Ladies Speak Out for Healthcare 2Health News:On Weekend Before the Election, New Jersey's First Ladies Speak Out for Healthcare 3Health News:Tailored Treatment Boosts Kidney Cancer Survival 2Health News:Suboxone Fast Passing Methadone As Preferred Method Of Treatment For Opiate Detox 2Health News:Landmark UCLA study finds aggresive, personalized treatment increases kidney cancer patient survival 2Health News:Landmark UCLA study finds aggresive, personalized treatment increases kidney cancer patient survival 3
(Date:9/19/2014)... , Sept. 19, 2014 Larry J. ... CVS ), will speak before the National Press Club ... discuss the company,s recent move to stop selling tobacco products ... the future of health. "Dramatic changes in our ... care providers and government," Merlo has said. "CVS Health has ...
(Date:9/19/2014)... 19, 2014  Pressure BioSciences, Inc. (OTCQB: PBIO) ... received and approved all parts required to build ... begun to manufacture the new PCT-based instrument system.  ... ready for shipment by mid-October and that additional ... a rate of about one per week thereafter.  ...
(Date:9/19/2014)... MINNEAPOLIS and ORLANDO, Fla. ... data supporting Inspire therapy continues to grow, with the ... paper published on-line in Otolaryngology – Head ... therapy causes a worsening of both objective and subjective ... to directly attribute Inspire therapy for the reduction in ...
Breaking Medicine Technology:CVS Health CEO to Address National Press Club on Landmark Decision to Quit Tobacco, Leadership in Health Care Innovation 2CVS Health CEO to Address National Press Club on Landmark Decision to Quit Tobacco, Leadership in Health Care Innovation 3Pressure BioSciences Announces Completion of the First Phase of Its Commercialization Plan for the Barozyme HT48, the Company's Recently Released High Throughput PCT-based Instrument System 2Pressure BioSciences Announces Completion of the First Phase of Its Commercialization Plan for the Barozyme HT48, the Company's Recently Released High Throughput PCT-based Instrument System 3New Data shows Reductions in obstructive sleep apnea (OSA) severity and improvement of quality of life with Inspire therapy 2New Data shows Reductions in obstructive sleep apnea (OSA) severity and improvement of quality of life with Inspire therapy 3New Data shows Reductions in obstructive sleep apnea (OSA) severity and improvement of quality of life with Inspire therapy 4
... LH Ovulation Predicting Test is a qualitative ... Hormone (hLH) in human urine to predict ... by the elevation of human luteinizing hormone ... period starts, hLH has a brief, significant ...
The Pach IV is the premier product for cornea thickness measurement in conjunction with Glaucoma screening....
... The Shandon Varistain 24-4 ... stainer designed to offer simplicity ... cytology staining applications. It is ... load and continuous throughput. ...
The Shandon Linistat Linear Stainer is a compact answer to H&E frozen section protocols, with a footprint that fits on top of most cryostas to streamline workflow during frozen sectioning....
Medicine Products: