Navigation Links
First validated method for analyzing flavanols and procyanidins in cocoa products could help scientists and the industry in standardized reporting
Date:8/30/2012

Mars, Incorporated, working in partnership with AOAC International, has successfully completed a multi-laboratory, first-of-its-kind validation of a method for analyzing flavanols and procyanidins in cocoa-based products. The study, just published in the latest edition of the Journal of AOAC International, details the results of a comprehensive evaluation of this method by 12 international laboratories, which included academic, industrial and commercial institutions. As it has been proved to be reproducible, robust, and readily transferable, this method could have far-reaching implications for researchers and consumers.

"This multi-laboratory collaborative study represents a critical breakthrough in the field by demonstrating that a method to measure these complex phytonutrients in cocoa can be reliable, robust, and easy-to-use," said Dr. Catherine Kwik-Uribe, study author and R&D Director at Mars Botanical. "Excitingly, in the not too distant future, this could lead to more uniform numbers on product labels that will help consumers compare and contrast flavanol-containing products, and help regulators evaluate claims."

Flavanols are a group of natural compounds that are particularly abundant in cocoa. Over the past decade, there has been growing interest in the potential cardiovascular and cognitive health benefits of flavanol and procyanidin-containing foods. For example, in July this year, the European Food Safety Authority (EFSA) issued a positive Scientific Opinion on the health benefits of cocoa flavanols and procyanidins related to healthy blood flow. Though these phytonutrients are of great scientific and consumer interest, until now there has been no common, validated approach to the analysis of these compounds in foods. As a result, different laboratories and companies have used different methods to report content. This has resulted in considerable variability and confusion in reported values, and scientists, regulators, and consumers lack clear and relevant information.

Moreover, the lack of an agreed method of analysis has left the field plagued by a variety of non-specific methods that provide no details about the types and mixtures of flavanols and procyanidins in foods, and typically overestimate the true levels of these compounds in foods, including cocoa products. Recognition of the limitations of these non-specific methods for reporting the content of phytonutrients has recently been highlighted by the USDA, which removed the USDA Oxygen Radical Absorbance Capacity (ORAC) Database for Selected Foods from its Nutrient Data Laboratory website. This was a result of mounting evidence indicating that measurements of "antioxidant capacity" have no relevance to human health, particularly as it relates to the benefits of specific phytonutrients.

The availability of a new, properly-validated and standardized method that provides specific compositional information on the types and mixtures of flavanols and procyanidins in cocoa and chocolate products is therefore an important step. Such a method will be crucial to establishing a causal relationship between the intake of the specific phytonutrients and their health benefits.

To address this, Mars, Incorporated initiated and led a collaborative study with AOAC International, a not-for-profit scientific association that assists in the validation of analytical methods worldwide. To validate the method at the various laboratories, scientists at Mars distributed 14 samples representing a variety of cocoa-containing products and ingredients with flavanol and procyanidin concentrations ranging from < 2 mg/g to 500 mg/g. These products included milk and dark chocolates, cocoa powders, cocoa liquors, and cocoa extracts. Using a liquid chromatographic method developed by Mars, published in 2009(1) and since improved, each lab tested all samples in duplicate for flavanols and procyanidins, and reported results. Subsequent evaluation of inter-laboratory performance showed each laboratory reported statistically consistent results, with variance ranging only between 4-10% for the samples. Reproducibility, a measure of the comparability of inter-laboratory performance, was strong, with less than 13% variance demonstrated for most samples.

The method is the culmination of a decade of work and represents the first multi-laboratory validated method for analyzing flavanols and procyanidins in cocoa. "Mars initiated its flavanol research program in the late 1980s and since then has developed significant expertise in the analysis of cocoa, which has been key to the program's success. Robust analytical methods such as the one just published enable the link between understanding the flavanol compounds in cocoa and the important benefits these components can have on our health. Without the proper analytical tools, this would be impossible," commented Harold Schmitz, Executive Director of the Mars Center for Cocoa Health Science.


'/>"/>
Contact: Marlene Machut
Marlene.Machut@mss.effem.com
301-444-7586
Mars, Incorporated
Source:Eurekalert

Related medicine news :

1. First targeted nanomedicine to enter human clinical studies
2. First contact: Early intervention key in diagnosis and treatment of serious mental illness
3. Donor Kidney Re-Used in Second Patient After Failing in First
4. Many Who First Misuse Prescription Pills Get Them From Friends, Family: Report
5. First-of-its-kind Menopause Map helps women navigate treatment
6. First recipients of AcademyHealths Presidential Scholarship announced
7. Kessler Foundation researchers present at first International Congress on Cognition in MS
8. Whos the Dad? First-Trimester Blood Test May Tell
9. AGA releases first independently developed ABIM-approved Practice Improvement Module in GI
10. Dr. Yael Mosse will receive first Nachman Award in Pediatric Oncology at national conference
11. First study investigating possible link between sunscreen ingredient and endometriosis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/23/2017)... ... January 23, 2017 , ... The Masters of Science in ... leaders with decades of experience who remain current practitioners in the field. With ... there is a renewed demand for versatile, data-driven leadership.The American University’s MS in ...
(Date:1/23/2017)... , ... January 23, 2017 , ... ... on Crystallization in Process Chemistry by Applying Simple PAT Tools . , ... compounds to isolate and purify the desired product. Chemists now spend more ...
(Date:1/23/2017)... ... 23, 2017 , ... Old School Labs™, makers of the wildly popular all-natural ... Breon Ansley to its growing team of brand ambassadors. The Olympia top finisher and ... less than a year was able to turn professional, participating in the 2013 NPC ...
(Date:1/23/2017)... ... January 23, 2017 , ... “Crossing the Bar”: a moving and eloquent ... life. “Crossing the Bar” is the creation of published author, Charlotte Hotte, a North ... who credits the inspiration of the book to her sister, Denise, wishes to acknowledge ...
(Date:1/23/2017)... ... 23, 2017 , ... Valentine’s Day is a time when many people celebrate romance and love ... may be looking for the ideal present, Atlanta-based Perimeter Plastic Surgery is offering ... free. Or, spend $200 and get $50 free. , “A lot of people just ...
Breaking Medicine News(10 mins):
(Date:1/23/2017)... 23, 2017 NeuroVive Pharmaceutical AB (Nasdaq ... a preclinical collaboration agreement with the Children,s Hospital of ... , M.D., a US key opinion leader in the mitochondrial ... ... compounds from NeuroVive,s research program, NVP015, in certain experimental disease ...
(Date:1/21/2017)... DIEGO , Jan. 20, 2017 ResMed (NYSE: ... 3B Medical ( Winter Haven, Florida ) today announced ... the parties. BMC and 3B will be permitted to sell their ... will make a one-time settlement payment to 3B to close the ... settlement did not include an admission of liability or wrongdoing by ...
(Date:1/21/2017)... & Faruqi, LLP, a leading national securities law firm, reminds ... KMPH ) of the federal securities class action lawsuit ... underwriters of the Company,s April 16, 2015 Initial Public Offering ... The lawsuit has been filed in the ... on behalf of all those who acquired KemPharm ...
Breaking Medicine Technology: