Navigation Links
First size-based chromatography technique for the study of livi
Date:4/22/2014

Using nanodot technology, Berkeley Lab researchers have demonstrated the first size-based form of chromatography that can be used to study the membranes of living cells. This unique physical approach to probing cellular membrane structures can reveal information critical to whether a cell lives or dies, remains normal or turns cancerous, that can't be obtained through conventional microscopy.

"We've developed membrane-embedded nanodot array platforms that provide a physical means to both probe and manipulate membrane assemblies, including signaling clusters, while they are functioning in the membrane of a living cell," says Jay Groves, a chemist with Berkeley Lab's Physical Biosciences Division, who led this research.

Groves, who is also a professor with the University of California (UC) Berkeley's Chemistry Department, and a Howard Hughes Medical Institute (HHMI) investigator, is a recognized leader in developing techniques for studying the impact of spatial patterns on living cells. The live-cell supported synthetic membranes he and his group have been developing are constructed out of lipids and assembled onto a substrate of solid silica. These membranes are being used to determine how living cells not only interact with their environment through chemical signals but also through physical force and spatial patterns.

"We call our approach the spatial mutation strategy because molecules in a cell can be spatially re-arranged without altering the cell in any other way," Groves says. "Our live cell-supported membranes provide a hybrid interface consisting of mobile and immobile components with controlled geometry that allows us to utilize solid-state nanotechnology to manipulate and control molecular systems inside living cells."

While the work of Groves and others in recent years has demonstrated the importance of protein and lipid spatial organization within cellular membranes, details as to how spatial organization is tied to function are scarce primarily because of the limitations of optical microscopy at length scales below the 250 nanometer diffraction limit. The size-based chromatography technique developed by Groves and his group allows them to probe supramolecular structures in a cell membrane at the needed nanometer length-scales.

"We now have a way to translate nano-sized structures that approach molecular dimensions into geometric constraints on the movement of molecules inside a living cell," Groves says.

For their size-based chromatography technique, the spacing of proteins and other cellular molecules is controlled by a hexagonal or honeycomb array of gold nanoparticles that is fabricated into the membrane. The spacing between nanoparticles in each array can be controlled, with accessible sizes ranging from 30 to nearly 200 nanometers.

"Individual membrane components move freely throughout the array, but movement of larger assemblies is impeded if they exceed the physical dimensions of the array, Groves says.

Groves and his colleagues tested their size-based chromatography technique on T cell receptor (TCR) microclusters in T cell membranes, which is the functional module for antigen recognition by T cells (lymphocytes from the thymus) in the body's immune response system. These TCR signaling clusters occupy a size regime ranging from tens to a few hundred nanometers, which is typically below the diffraction limit of conventional optical microscopy. Size-based chromatography was used to probe the physical properties of TCR signaling clusters as a function of antigen density. The results revealed that TCR signaling cluster is distinctly dependent on the amount of antigen encountered by the cell.

"This is something we did not know before about the TCR microcluster signaling system, which has been well-studied using conventional optical microscopy," Groves says. "It is a proof-of-principle demonstration that represents another step in the direction of interfacing living cells with synthetic materials to achieve molecular level control of the cell."


'/>"/>
Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Related medicine news :

1. First targeted nanomedicine to enter human clinical studies
2. First contact: Early intervention key in diagnosis and treatment of serious mental illness
3. Donor Kidney Re-Used in Second Patient After Failing in First
4. Many Who First Misuse Prescription Pills Get Them From Friends, Family: Report
5. First-of-its-kind Menopause Map helps women navigate treatment
6. First recipients of AcademyHealths Presidential Scholarship announced
7. Kessler Foundation researchers present at first International Congress on Cognition in MS
8. Whos the Dad? First-Trimester Blood Test May Tell
9. AGA releases first independently developed ABIM-approved Practice Improvement Module in GI
10. Dr. Yael Mosse will receive first Nachman Award in Pediatric Oncology at national conference
11. First study investigating possible link between sunscreen ingredient and endometriosis
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
First size-based chromatography technique for the study of livi
(Date:12/2/2016)... ... 2016 , ... CloudLIMS.com, a class-leading provider of Laboratory Management ... Lite helps biobanks, clinical, research and testing laboratories keep track of their biospecimens, ... is a faster and a more efficient product, allowing batch processing of data, ...
(Date:12/2/2016)... West Milford, NJ (PRWEB) , ... December 02, 2016 , ... ... Norway, Nothing Water has some unique properties including its unmatched natural purity of just ... described as clean and crisp. , Nothing Water has been available in several ShopRite ...
(Date:12/2/2016)... , ... December 02, 2016 , ... "Pro3rd Accents Volume ... editors to create versatile lower third titles with just a few clicks of the ... Volume 2 includes 30 lower third animations. Choose from various styles with accented animations, ...
(Date:12/2/2016)... (PRWEB) , ... December 02, 2016 , ... ... pharmaceutical organizations to build intelligent, connected applications, was named the best Sales Team ... , The winner announcement was made today by the Software & Information Industry ...
(Date:11/30/2016)... ... November 30, 2016 , ... The Fertility ... designated as a Cigna Infertility Center of Excellence. The Cigna Center of Excellence ... “It’s an honor to be designated a Cigna Infertility Center of Excellence," said ...
Breaking Medicine News(10 mins):
(Date:12/2/2016)... 2016  PipelineRx, a leading medication management technology ... demonstrations of its SaaS-based telepharmacy platform, PowerGridRx™ ... 2016 Midyear Clinical Meeting and Exhibition, December 5-7 ... nearly 300 hospital clients nationally, the Company is ... to dramatically improve pharmacy operations, enhance patient safety, ...
(Date:12/2/2016)... On Thursday, December 1st 2016, the Prix ... and innovation in the biopharmaceutical industry at its third ... presence of Sergey Tsyb, Vice Minister of Industry and ... Natalia Sanina, First Vice Chairman of the State Duma ... National Service of Control in Healthcare, Sergey Muravev, Director ...
(Date:12/2/2016)... , Dec. 2, 2016  Eli Lilly and ... financial guidance for 2017 and provide updated financial guidance ... also conduct a conference call on that day with ... company,s financial guidance. The conference call will ... general public can access a live webcast of the ...
Breaking Medicine Technology: