Navigation Links
Faster, scalable method for producing AAV-based gene transfer vectors
Date:1/5/2011

New Rochelle, NY, January 5, 2011A new, simplified method for producing large amounts of viral vector cassettes capable of shuttling genes into host cells will help advance the promising field of gene therapy as applications move into large animal studies and human clinical trials. The novel adeno-associated virus (AAV) production method is described in an article published Instant Online ahead of publication in Human Gene Therapy, a peer-reviewed journal published by Mary Ann Liebert, Inc. (www.liebertpub.com). The article is available free online at www.liebertpub.com/hum

This new method for creating stable AAV2 producer cell lines overcomes the major drawbacks of the previous method: it requires only a one-step cloning process (instead of multiple cloning steps), and a single (instead of two-stage) transfection and selection step to produce cell lines containing the circular, AAV vector-containing plasmid DNA needed to transport a gene of interest into host cells.

Zhenhua Yuan, Chunping Qiao, Peiqi Hu, Juan Li, and Xiao Xiao, researchers at the Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, state that in addition to being faster and easier, their simplified method offers other important advantages, including the potential to produce high yields of multiple AAV serotypes upon co-infection with a helper adenovirus, as well as efficient packaging of single- or double-stranded AAV vectors and of large AAV cassettes. The authors describe their method in the article entitled, "A Versatile AAV Producer Cell Line Method for Scalable Vector Production of Different Serotypes."

"AAV gene therapy is showing promise in the clinic. Important challenges exist for it to become a commercial product such as scalable manufacturing processes. The study by Yuan et al. is an important step toward the development of a method of production that is capable of supporting a commercial product," says James M. Wilson, MD, PhD, Editor-in-Chief, and Director of the Gene Therapy Program, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia.


'/>"/>

Contact: Vicki Cohn
vcohn@liebertpub.com
914-740-2156
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert  

Related medicine news :

1. Minimally invasive sports hernia repair may get athletes back in the game faster, study says
2. Second-line CML drugs evoke faster, better front-line remissions
3. Northridge Dentist, Dr. Ariz, Uses Latest Technological Advances to Give His Patients Faster, Painless Treatment
4. De-Duplication, Compression and No Double Backups Required: AppAssure Releases the Fastest and Most Scalable Backup & Recovery Solution for Microsoft Hyper-V
5. Infosolve Technologies Demonstrates OpenDQ's Massively Scalable Fuzzy Matching Capabilities on a Cloud Platform
6. Live with Regis and Kelly Reveal A REVOLUTIONARY Pain Relief Method
7. Two Surgical Methods Equally Successful for Prostate Cancer
8. Psychiatrys main method to prevent mistaken diagnoses of depression doesnt work: NYU study
9. New testing method hints at garlics cancer-fighting potential
10. MSU scientists develop more effective method of predicting lead-poisoning risk
11. Blood Draw Dilemma: New Summit Health Article Helps Employers Choose the Best Screening Method
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Faster, scalable method for producing AAV-based gene transfer vectors
(Date:6/27/2016)... ... ... TherapySites, the leading website and online marketing ... Association. This new relationship allows TherapySites to continue to extend their online ... and promotional offers. , "TCA is extremely excited about this new partnership, as ...
(Date:6/27/2016)... ... June 27, 2016 , ... TopConsumerReviews.com recently awarded their highest five-star rating to ... of individuals in the United States and Canada wear eyeglasses. Once considered to be ... vision and make a fashion statement. Even celebrities use glasses as a way of ...
(Date:6/26/2016)... ... ... Pixel Film Studios Released ProSlice Levels, a Media Slicing Effect Plugin ... whole new perspective by using the title layers in ProSlice Levels to split-up ... Levels contains over 30 Different presets to choose from. FCPX users can ...
(Date:6/26/2016)... ... June 26, 2016 , ... Many women are ... with endometriosis. These women need a treatment plan to not only alleviate symptoms ... can help for preservation of fertility and ultimately achieving a pregnancy. The specialists ...
(Date:6/25/2016)... ... ... temporary closing of Bruton Memorial Library on June 21 due to a possible lice infestation, ... aspect of head lice: the parasite’s ability to live away from a human host, and ... necessary one in the event that lice have simply gotten out of control. , As ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 Roche (SIX: ... 510(k) clearance for its Elecsys BRAHMS PCT (procalcitonin) assay ... sepsis or septic shock. With this clearance, Roche is ... a fully integrated solution for sepsis risk assessment and ... with bacterial infection and PCT levels in blood can ...
(Date:6/23/2016)... , June 23, 2016 Bracket , ... launch its next generation clinical outcomes platform, Bracket eCOA (SM) ... held on June 26 – 30, 2016 in ... first electronic Clinical Outcome Assessment product of its kind to ... #715. Bracket eCOA 6.0 is a flexible platform ...
(Date:6/23/2016)... LONDON , June 23, 2016 ... environments  Oticon , industry leaders in ... the launch of Oticon Opn ™, the world,s ... world of possibilities for IoT devices.      ... Opn, Oticon introduces a number of ,world firsts,: ...
Breaking Medicine Technology: