Navigation Links
Faster, scalable method for producing AAV-based gene transfer vectors
Date:1/5/2011

New Rochelle, NY, January 5, 2011A new, simplified method for producing large amounts of viral vector cassettes capable of shuttling genes into host cells will help advance the promising field of gene therapy as applications move into large animal studies and human clinical trials. The novel adeno-associated virus (AAV) production method is described in an article published Instant Online ahead of publication in Human Gene Therapy, a peer-reviewed journal published by Mary Ann Liebert, Inc. (www.liebertpub.com). The article is available free online at www.liebertpub.com/hum

This new method for creating stable AAV2 producer cell lines overcomes the major drawbacks of the previous method: it requires only a one-step cloning process (instead of multiple cloning steps), and a single (instead of two-stage) transfection and selection step to produce cell lines containing the circular, AAV vector-containing plasmid DNA needed to transport a gene of interest into host cells.

Zhenhua Yuan, Chunping Qiao, Peiqi Hu, Juan Li, and Xiao Xiao, researchers at the Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, state that in addition to being faster and easier, their simplified method offers other important advantages, including the potential to produce high yields of multiple AAV serotypes upon co-infection with a helper adenovirus, as well as efficient packaging of single- or double-stranded AAV vectors and of large AAV cassettes. The authors describe their method in the article entitled, "A Versatile AAV Producer Cell Line Method for Scalable Vector Production of Different Serotypes."

"AAV gene therapy is showing promise in the clinic. Important challenges exist for it to become a commercial product such as scalable manufacturing processes. The study by Yuan et al. is an important step toward the development of a method of production that is capable of supporting a commercial product," says James M. Wilson, MD, PhD, Editor-in-Chief, and Director of the Gene Therapy Program, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia.


'/>"/>

Contact: Vicki Cohn
vcohn@liebertpub.com
914-740-2156
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert  

Related medicine news :

1. Minimally invasive sports hernia repair may get athletes back in the game faster, study says
2. Second-line CML drugs evoke faster, better front-line remissions
3. Northridge Dentist, Dr. Ariz, Uses Latest Technological Advances to Give His Patients Faster, Painless Treatment
4. De-Duplication, Compression and No Double Backups Required: AppAssure Releases the Fastest and Most Scalable Backup & Recovery Solution for Microsoft Hyper-V
5. Infosolve Technologies Demonstrates OpenDQ's Massively Scalable Fuzzy Matching Capabilities on a Cloud Platform
6. Live with Regis and Kelly Reveal A REVOLUTIONARY Pain Relief Method
7. Two Surgical Methods Equally Successful for Prostate Cancer
8. Psychiatrys main method to prevent mistaken diagnoses of depression doesnt work: NYU study
9. New testing method hints at garlics cancer-fighting potential
10. MSU scientists develop more effective method of predicting lead-poisoning risk
11. Blood Draw Dilemma: New Summit Health Article Helps Employers Choose the Best Screening Method
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Faster, scalable method for producing AAV-based gene transfer vectors
(Date:10/13/2017)... ... , ... As health professionals work to improve their approach to healthcare, there ... more than filling out a survey; in many cases health professionals and patients are ... care and research on the importance of active engagement with patients and members of ...
(Date:10/13/2017)... ... October 13, 2017 , ... ... Pharmacy (SOP) alumni Hannah Randall, PharmD ‘17, and Jennifer Huggins, PharmD ’17, ... guideline updates for the primary prevention of cardiovascular diseases during the 15th ...
(Date:10/13/2017)... ... October 13, 2017 , ... Ellevate Network, the leading network for professional women, ... towards gender equality at their inaugural Summit in New York City in June. The ... social audience of over 3 million. To watch the Mobilize Women video, click ...
(Date:10/13/2017)... (PRWEB) , ... October 13, 2017 , ... “The Journey: ... faced every danger possible to save lost souls in the Philippines. “The Journey: From ... Carole is a dedicated teacher of the Bible. She has taught all ages and ...
(Date:10/12/2017)... ... October 12, 2017 , ... The ... demand of today’s consumer and regulatory authorities worldwide. From Children’s to Adults 50+, ... to meet the highest standard. , These products are also: Gluten Free, ...
Breaking Medicine News(10 mins):
(Date:10/7/2017)... IRVING, Texas , Oct. 6, 2017   ... industry with more than $100 billion in purchasing power, ... industry news and information. The Newsroom is ... chain and industry trends, infographics, expert bios, news releases, ... Besides having access to a wealth of resources at ...
(Date:10/2/2017)... -- Diplomat Pharmacy, Inc. (NYSE: DPLO ) ... , and named its founder as Diplomat,s chief information ... Tennessee , will operate under Diplomat subsidiary Envoy ... for health care partners to include IT outsourcing, consulting, ... "In an interoperable world, technology delivers comprehensive insight and ...
(Date:9/28/2017)... , Sept. 28, 2017 Hill-Rom Holdings, ... 2017 earnings conference call and webcast on Friday, November ... a.m. (EDT) and ending at approximately 8:30 a.m. (CDT) ... discussing the company,s 2017 financial performance and guidance for ... opportunities, initiatives to enhance operational performance, and long-range financial ...
Breaking Medicine Technology: