Navigation Links
Experimental technique at GUMC offers real-time analysis of breast cancer biopsies
Date:11/13/2007

Washington, D.C. − A sophisticated microscope that offers a real-time 3-D analysis of tissue samples might, in the future, reduce the number of needle biopsies traditionally needed from women suspected of having breast cancer, according to recent research published at Georgetown University Medical Centers Lombardi Comprehensive Cancer Center.

Such an instant test would mean that physicians could immediately tell if they have collected adequate samples of breast tissue and limit the number of repeat biopsies, said the investigators, whose study appeared in the September/October issue of the Journal of Biomedical Optics.

Currently, physicians extract 6-8 tissue samples during a needle biopsy procedure to ensure proper sampling of the area of concern. In addition, at least one day is required to prepare the samples for analysis using traditional methods. The new technology is designed to limit patient discomfort and anxiety.

With this microscope, we can tell instantly whether we have cancer cells or not, or what kind of cells we are looking at, whether they are fat, structural, or epithelial cells that line breast milk ductsall of this could give us a great advantage in treating breast cancer, said the studys lead investigator, Maddalena T. Tilli, PhD, a postdoctoral fellow in the laboratory of Priscilla Furth, MD, at the Lombardi Comprehensive Cancer Center, part of Georgetown University Medical Center.

This tool could perhaps be used at the bedside to not only reduce the number of biopsies that we take from patients, but which could possibly be used during an operation to make sure surgeons have removed all cancerous tissue, said Furth, the studys senior investigator and a professor of oncology at Georgetown.

The technique is fairly simple. A reflectance confocal microscope reflects near-infrared light off multiple mirrors through tissue, then records the scattering of light. The idea behind this is that certain structures in the cell will look different based on the pattern of light reflectance, Tilli said. To highlight the nuclei in the cell, the researchers douse the tissue in a 5 percent solution of acetic acid, the major component in vinegar. The acid condenses DNA in the cell, and based on where the DNA is located, you can tell what kind of cells these are, she says.

The whole procedure takes 10 minutes or less from the time the tissue is removed, researchers say. Traditionally, biopsy tissue is fixed in a solution of formaldehyde to preserve it, then it is embedded in wax and, finally, sliced and stained. While this technique reveals a lot of detail about cells, it takes a long time to prepare, Furth said. Now we can combine both of these methods, she added. We can sample biopsy tissue instantly with microscopy and then use the same sample to fix it in the normal way to assess the type of cancer a woman has.

Funded by grants from Susan G. Komen For the Cure, awarded to Georgetown breast cancer oncologist and principal investigator Minetta C. Liu, MD, Tilli and Furth adapted the technique for analyzing reading breast tissue. Reflectance confocal microscopy also is currently being used on a limited scale to assess skin conditions such as melanoma.

Tilli and Furth first determined that the mechanism worked well to image all stages of normal mammary gland development in animal models. Then they examined 25 breast needle biopsies taken from 16 patients to look at whether reflectance confocal microscopy can assess, in real time, the content of epithelial cells and supporting structural stromal cells as well as the presence or absence of cancer, in comparison to the same biopsy tissue prepared in the traditional way.

We found that we can tell the difference between cells, and we can determine if a cell is cancerous, but we cant yet tell precisely which type of cancer is present, Tilli said. Another advantage this procedure offers us is that we can look at different layers in the tissue, unlike the traditional method, which slices tissue apart.

The researchers are currently studying use of new contrast agents and other methods to increase detection and resolution of cancer cells.


'/>"/>

Contact: Becky Wexler
rjw43@georgetown.edu
202-687-5100
Georgetown University Medical Center
Source:Eurekalert

Related medicine news :

1. Experimental anti-cancer drug made from corn lillies kills brain tumor stem cells
2. Experimental Antidepressants Offer Faster Relief
3. Experimental drug shows promise in advanced kidney cancer
4. Experimental drug boosts survival in recurrent ovarian cancer
5. Experimental cancer pharmaceuticals under trial
6. Experimental Drug Fails Against Heart Failure
7. Experimental Drug Fights Prostate Cancer
8. Eye-staining technique offers early detection for dry eye syndrome
9. New technique detects specific chromosomal damage, may indicate lung cancer risk
10. The Pharma & Life Sciences Best Practice Database: an Industry Resource for Successful Techniques and Performance Benchmarks for Reaching the Top
11. Latest DES Analysis Stresses Importance of Physicians Well-Trained in Implantation Technique and Patient Follow-Up
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2016)... , ... June 24, 2016 , ... Those who have ... these feelings, many turn to unhealthy avenues, such as drug or alcohol abuse, as ... Michigan, has released tools for healthy coping following a traumatic event. , Trauma sufferers ...
(Date:6/24/2016)... ... June 24, 2016 , ... Marcy was in a crisis. Her son James, eight, was out ... family verbally and physically. , “When something upset him, he couldn’t control his emotions,” remembers ... would throw rocks at my other children and say he was going to kill them. ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Global law ... magazine’s 2016 Legal Elite. The attorneys chosen by their peers for this recognition are ... , Seven Greenberg Traurig Shareholders received special honors as members of this year’s Legal ...
(Date:6/24/2016)... ... June 24, 2016 , ... Comfort Keepers® of San Diego, CA is ... Road To Recovery® program to drive cancer patients to and from their cancer treatments. ... the highest quality of life and ongoing independence. Getting to and from medical ...
(Date:6/24/2016)... ... 24, 2016 , ... People across the U.S. are sharpening their pencils and ... essay contest in which patients and their families pay tribute to a genetic counselor ... National Society of Genetic Counselors (NSGC) Annual Education Conference (AEC) this September. , ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... Calif. , June 24, 2016  American Respiratory Labs (ARL), ... is now able to perform sophisticated lung assessments in patients, homes, ... , Inc. Patients are no longer limited to ... EasyOne PRO ® , ARL patients like Jeanne R. of ... in the comfort of her own home. ...
(Date:6/24/2016)... India , June 24, 2016 ... Needles Market by Type (Standard Pen Needles, Safety Pen ... Therapy (Insulin, GLP-1, Growth Hormone), Mode of Purchase (Retail, ... by MarketsandMarkets, This report studies the market for the ... expected to reach USD 2.81 Billion by 2021 from ...
(Date:6/24/2016)... , June 24, 2016 ... appointment of Dr. Edward Futcher to ... Director, effective June 23, 2016.Dr. Futcher was also ... and Governance Committees.  As a non-executive member of ... expertise and strategic counsel to VolitionRx in connection ...
Breaking Medicine Technology: