Navigation Links
Estrogen and progesterone receptor isoforms expression in the stomach of Mongolian gerbils
Date:10/31/2008

We have previously shown that E2 and P4 have clear and distinct effects on inflammatory response and gastric epithelial changes during early H. pylori infection. Recently, Ohtani et al., have shown a protective role of E2 administration in H. pylori-infected InGas mice. Other studies have demonstrated that E2 and P4 have anti-ulcerative effects in murine gastric mucosa. ER and PR have been reported in human, mouse and rat stomach. However, there is no information to date of their expression and regulation in Mongolian gerbil stomach.

Ovariectomized adult female gerbils were subcutaneously treated with E2, and E2+P4. Uteri and stomachs were removed, the latter were cut along the greater curvature, and antrum and corpus were excised. Proteins were immunoblotted using antibodies that recognize ER-alpha, ER-beta, and PR-A and PR-B receptor isoforms. Tissues from rats treated in the same way were used as control.

Specific bands were detected for ER-alpha (68 kDa), and PR isoforms (85 and 120 kDa for PR-A and PR-B isoforms, respectively) in uteri, gastric antrum and corpus. We could not detect ER-beta isoform. PR isoforms were not regulated by E2 or P4 in uterus and gastric tissues of gerbils. ER-alpha isoform content was significantly down-regulated by E2 in the corpus, but not affected by hormones in uterus and gastric antrum. We have detected ER-alpha and both PR isoforms in gerbil gastric antrum and corpus. In the antrum, none of the receptors was regulated by E2 and P4, but in gastric corpus we observed a down-regulation by E2. Thus, our results show that ER-alpha regulation also depends on the type of cells present in the mucosa. The mechanisms involved in the lack of regulation of PR in gerbil stomach, as well as the differential regulation of ER-alpha by E2 deserve further research. The presence of ER-alpha and PR isoforms in gerbil stomach suggests that E2 and P4 actions in this organ are in part mediated by their nuclear receptors.

A research article to be published on October 7,2008 in the World Journal of Gastroenterology addresses this question. The research team led by Dr. Camacho-Arroyo from Universidad Nacional Autnoma de Mxico used commercially available antibodies to detect estrogen and progesterone receptors isoforms in female gerbil stomach. They also evaluated the effects of E2 and E2+P4 in the regulation of these receptors. As it has been previously reported that stomach has steroidogenic activity, and that both estradiol (E2) and progesterone (P4) play differential roles in response to gastric injury and H. pylori infection, it was important to be able to detect these proteins in Mongolian gerbils stomach.

Specific bands for estrogen receptor (ER)-alpha (68 kDa), progesterone receptor (PR)-A (85 kDa) and PR-B (120 kDa) were detected, unfortunately they were unable to detect ER-beta isoform. The identity of the receptors was defined by using rat uteri samples along with the Mongolian gerbil uteri. This is important because Mongolian gerbils sex steroid receptors have not been sequenced at the gene or protein levels. The detection of these receptors with antibodies raised against other specie receptors suggests high conservation of the specific epitopes among species. The validation of commercially available antibodies for ER and PR in Mongolian gerbils adds applicability to the results of this study.

The regulation of ER and PR by E2 and P4 was first defined in target organs in the reproductive tract, and after several studies, there is enough evidence that this regulation is tissue specific and it is associated to the function of E2 and P4 in every particular tissue. ER and PR expression regulation in Mongolian gerbils has not been reported previously. The lack of regulation of ER and PR in gerbil uteri deserved further investigation. The differential regulation in gastric antrum and corpus suggests differential roles of these receptors in the different gastric mucosa. This study raises interesting questions to be addressed in future research.


'/>"/>

Contact: Lin Tian
wjg@wjgnet.com
86-105-908-0039
World Journal of Gastroenterology
Source:Eurekalert

Related medicine news :

1. Estrogen flooding our rivers, Université de Montréal study
2. Estrogen Cream No Match for Sun-Damaged Skin
3. Estrogen May Have Preventive Role in Womens Schizophrenia
4. Team discovers new inhibitors of estrogen-dependent breast cancer cells
5. Estrogen therapy helps or hurts the brain depending on reproductive status
6. Anti-estrogen drug therapy reduces risk of invasive breast cancer in older women
7. Previously unseen switch regulates breast cancer response to estrogen
8. Estrogen therapy increases benign breast disease risk
9. Estrogen Supplements May Raise Odds of Benign Breast Disease
10. New study finds adverse effects of estrogen replacement therapy are related to the dose
11. Mitochondria play role in pathogenesis of AD and estrogen-induced neuroprotection
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2017)... ... April 24, 2017 , ... ... Bring Your Own Device (BYOD) capabilities at Telehealth 2.0, the American Telemedicine Association’s ... bundles, which pairs medical devices with a pre-programmed tablet in a remarkably easy-to-use ...
(Date:4/24/2017)... ... , ... Ridgecrest Herbals makes it a point to lead the Utah supplement ... support renewable energy. They believe this is a crucial part of their mission as ... issues, and maintain that destroying the environment in the pursuit of profit is unsustainable ...
(Date:4/24/2017)... ... 2017 , ... The bar for just about everything—apparel, eyewear, cars and even ... of pop and film stardom.(1) Not to be left out is that most desired ... run the gamut from being encrusted with jewels and precious metals to, at the ...
(Date:4/24/2017)... (PRWEB) , ... April 24, 2017 , ... Sean Fay ... Oxiclean, Space Bag, Sonicare Toothbrush, Juiceman Juicer, and the George Foreman Grill (which sold ... any other marketer in the last 25 years. , Now, due to changes in ...
(Date:4/24/2017)... ... April 24, 2017 , ... ALP Nutrition®, a ... announced its popular products are now available for purchase on StackedNutrition.com, a popular ... use of premium natural ingredients in making all of its products. These ingredients ...
Breaking Medicine News(10 mins):
(Date:4/20/2017)... 20, 2017 Research and Markets has ... Market Share, Application Analysis, Regional Outlook, Growth Trends, Key Players, ... their offering. ... pharmacogenomics market was valued at US$ 7,167.6 Mn in 2015, ... expanding at a CAGR of 5.6% from 2016 to 2024. ...
(Date:4/20/2017)... , April 20, 2017 Research and ... Pharmaceutical Contract Manufacturing Services Market Analysis By Service (Manufacturing, Research), ... Forecasts, 2014 - 2025" report to their offering. ... The Latin American pharmaceutical ... billion by 2025 Low drug registration cost in ...
(Date:4/19/2017)... The Mobile X-Ray product segment is the most attractive market ... period Mobile X-Ray segment is the largest segment ... market, which is estimated to be valued at more than ... 7% over the forecast period. Mobile X-Ray segment is expected ... Mn in 2017 over 2016. The segment,s revenue in the ...
Breaking Medicine Technology: