Navigation Links
Enhancing RNA interference
Date:6/24/2013

ngulf large molecules. The MIT team found that once the nanoparticles enter cells they become trapped in bubbles known as endocytic vesicles. This prevents most of the siRNA from reaching its target mRNA, which is located in the cell's cytosol (the main body of the cell).

This happens even with the most effective siRNA delivery materials, suggesting that there is a lot of room to improve the delivery rate, Anderson says.

"We believe that these particles can be made more efficient. They're already very efficient, to the point where micrograms of drug per kilogram of animal can work, but these types of studies give us clues as to how to improve performance," Anderson says.

Molecular traffic jam

The researchers found that once cells absorb the lipid-RNA nanoparticles, they are broken down within about an hour and excreted from the cells.

They also identified a protein called Niemann Pick type C1 (NPC1) as one of the major factors in the nanoparticle-recycling process. Without this protein, the particles could not be excreted from the cells, giving the siRNA more time to reach its targets. "In the absence of the NPC1, there's a traffic jam, and siRNA gets more time to escape from that traffic jam because there is a backlog," says Gaurav Sahay, an MIT postdoc and lead author of the Nature Biotechnology paper.

In studies of cells grown in the lab without NPC1, the researchers found that the level of gene silencing achieved with RNA interference was 10 to 15 times greater than that in normal cells.

Lack of NPC1 also causes a rare lysosomal storage disorder that is usually fatal in childhood. The findings suggest that patients with this disorder might benefit greatly from potential RNA interference therapy delivered by this type of nanoparticle, the researchers say. They are now planning to study the effects of knocking out the NPC1 gene on siRNA delivery in animals, with an eye toward testing possible si
'/>"/>

Contact: Sarah McDonnell
s_mcd@mit.edu
617-253-8923
Massachusetts Institute of Technology
Source:Eurekalert

Page: 1 2 3

Related medicine news :

1. Enhancing Compatibility and Boosting Performance; NEXCOM Introduces the ICES 670 COM Express Module Based on 4th Generation Intel® Core™ Processor
2. Research Suggests that Lack of Exercise Drive is Genetic, Making Energy Enhancing Supplements Like Prescopodene Beneficial For Sticking to Your Exercise Routine
3. NYU and NYU Langone researchers devise method for enhancing CEST MRI
4. Researchers identify new pathway, enhancing tamoxifen to tame aggressive breast cancer
5. BOMI International and Transwestern Partner to Offer Property and Facility Staff Skill-Enhancing Education Programs
6. Continuing Education Provider HomeCEUConnection.com Adds New Career Enhancing Video Courses to Online Catalog
7. Delray Recovery Center Treats Steroid and Performance Enhancing Drug Abuse
8. Astro Clairvoyant Norah Guide for Premium Astrology Announces Tools for Enhancing Inner Psychic Abilities in Blog Series
9. Enhancing breast cancer detection
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/16/2014)... September 16, 2014 Not all recovery ... take care to make sure all their patients are ... some, this may include the newly-introduced online therapy. Millennium ... which gives patients the opportunity to seek treatment from ... provide medical and clinical care at a distance. They ...
(Date:9/16/2014)... WA (PRWEB) September 16, 2014 Duke’s Chowder ... Seattle startups Rebls and Gratafy for a ... and startup hub is firmly cemented. With the growth of companies ... of opportunities for people who work in tech and at startups ... Rebls and Gratafy are happy to host events for their peers ...
(Date:9/16/2014)... A new clinical trial comparing the ... balloons (DEB) in treating in-stent restenosis (ISR) ... superior late angiographic results and better late ... today at the 26th annual Transcatheter Cardiovascular ... Cardiovascular Research Foundation (CRF), TCT is the ...
(Date:9/16/2014)... 16, 2014 Programs and policies that ... of billions of dollars in savings for the US ... Commonwealth Fund. To help spur these types of improvements, ... 10 collaborative groups from across the country to field ... ) - a handbook designed to assist groups working ...
(Date:9/16/2014)... AcousticSheep LLC, makers of the most ... Wireless, their latest version of RunPhones® headphones. RunPhones® Wireless ... active people seeking a safe, comfortable headphone. They ... market and will be available October 1, 2014. ... of the same great features as its predecessor, RunPhones® ...
Breaking Medicine News(10 mins):Health News:Millennium Treatment Group Now Offers Online Therapy 2Health News:Seattle Seafood Restaurant Duke’s Chowder House Partners with Rebls & Gratafy for Tech Networking Happy Hour Event. 2Health News:Seattle Seafood Restaurant Duke’s Chowder House Partners with Rebls & Gratafy for Tech Networking Happy Hour Event. 3Health News:Results of RIBS IV trial reported at TCT 2014 2Health News:NQF Seeks Field Testing Groups for Population Health Guide 2Health News:NQF Seeks Field Testing Groups for Population Health Guide 3Health News:RunPhones® Prepares to Launch Their New Highly Anticipated Wireless Headphones 2Health News:RunPhones® Prepares to Launch Their New Highly Anticipated Wireless Headphones 3
... The Consumer-Purchaser,Disclosure Project --- a group of leading ... all Americans have access to publicly,reported health care ... General of New York and Cigna Healthcare to ... will,allow physician performance measurement and reporting to proceed ...
... Remind Me Today! reminder program, TORONTO, Oct. 29 ... program - a free telephone-based reminder program designed to,help ... Canada, over 1.3 million women (Stat. Can., 2001) take ... who hasn,t,forgotten to take it at least once. Although ...
... Key Element To An Effective, Hygiene Protocol. ... WASHINGTON, Oct. 29 The recent outbreak ... as an important,reminder of the importance of routine, ... large number of people constantly live,work or play. ...
... may affect heart despite symptom relief, experts say , , ... live longer than they did 40 years ago, but not ... from the Mayo Clinic. , "When you look at persons ... the benefits over the last several decades of improved survival ...
... Patients with chronic tendinosis of the Achilles tendon can ... small amount of a dextrose solution, according to a ... in Vancouver, BC. , Chronic tendinosis is a common ... and can affect many tendons throughout the body, said ...
... announced results from a recent economic analysis of Synagis ... of care for prevention of severe lower respiratory tract ... infants. Released at the American Academy of Pediatrics (AAP) ... costs of providing Synagis to premature infants, as well ...
Cached Medicine News:Health News:Consumers, Health Care Purchasers Applaud Agreement On Physician Performance Reporting 2Health News:Consumers, Health Care Purchasers Applaud Agreement On Physician Performance Reporting 3Health News:Consumers, Health Care Purchasers Applaud Agreement On Physician Performance Reporting 4Health News:Canada leads the way in contraceptive compliance 2Health News:Canada leads the way in contraceptive compliance 3Health News:Spate of Staph Infections Highlight the Need for Thorough, Consistent Cleaning Regimen at Home, at School and at Work 2Health News:Rheumatoid Arthritis Death Rate Unchanged 2Health News:Rheumatoid Arthritis Death Rate Unchanged 3Health News:Ultrasound-guided injections help ease chronic Achilles tendon pain 2
(Date:9/16/2014)... 16, 2014 Research and Markets ... "Global Laparoscopy Devices Industry Report 2014" report ... Laparoscopy Devices Industry Report 2014 is a professional ... the global laparoscopic devices. The report ... definitions, classifications, applications and industry chain structure. The ...
(Date:9/16/2014)... Sept. 16, 2014 Based on its ... Frost & Sullivan recognises Incisive Surgical with the ... Value Leadership. Incisive Surgical,s uniquely-designed, innovative INSORB® Absorbable ... eliminates the need for patients to undergo the ... removal  that may result in increased pain, wound ...
(Date:9/16/2014)... DIEGO , Sept. 16, 2014 Imprimis ... pharmaceutical company dedicated to delivering high quality and novel ... announced that Chief Executive Officer Mark Baum ... Annual Craig-Hallum Alpha Select Conference in New York City.  ... its Go Dropless™ campaign in ophthalmology. The presentation ...
Breaking Medicine Technology:Global Laparoscopy Devices Industry Report 2014 2Frost & Sullivan Commends Incisive Surgical for Delivering Superior Customer Value through its Patient-Centric, Cost-Effective INSORB Absorbable Stapler 2Frost & Sullivan Commends Incisive Surgical for Delivering Superior Customer Value through its Patient-Centric, Cost-Effective INSORB Absorbable Stapler 3Frost & Sullivan Commends Incisive Surgical for Delivering Superior Customer Value through its Patient-Centric, Cost-Effective INSORB Absorbable Stapler 4Frost & Sullivan Commends Incisive Surgical for Delivering Superior Customer Value through its Patient-Centric, Cost-Effective INSORB Absorbable Stapler 5Imprimis Pharmaceuticals to Present at Craig-Hallum Alpha Select Conference 2Imprimis Pharmaceuticals to Present at Craig-Hallum Alpha Select Conference 3Imprimis Pharmaceuticals to Present at Craig-Hallum Alpha Select Conference 4
... Md., Aug. 1, 2011 /PRNewswire-USNewswire/ – CoMeD ... Nations Environment Programme (UNEP) distributed a revised ...  Advocates for mercury-free drugs were gratified to see pharmaceuticals ... the proposed treaty. The Coalition for ...
... 2011 Reportlinker.com announces that a new ... catalogue: Patient Self-Monitoring: Devices, ...   http://www.reportlinker.com/p0581171/Patient-Self-Monitoring-Devices-Markets-Players-and-Prospects .html#utm_source=prnewswire&utm_medium=pr&utm_campaign=In_Vitro_Diagnostic ... monitor their own serum levels is deemed ...
Cached Medicine Technology:Global Mercury Treaty May Include Ban on Mercury in Medicine 2Global Mercury Treaty May Include Ban on Mercury in Medicine 3Patient Self-Monitoring: Devices, Markets, Players and Prospects 2Patient Self-Monitoring: Devices, Markets, Players and Prospects 3
... ARUP Laboratories is a national reference ... laboratory research and development. ARUP offers an ... unique medical tests in clinical and anatomic ... ARUP Laboratories' clients include more than half ...
... ARUP Laboratories is a national reference ... laboratory research and development. ARUP offers an ... unique medical tests in clinical and anatomic ... ARUP Laboratories' clients include more than half ...
... ARUP Laboratories is a national reference laboratory ... research and development. ARUP offers an extensive ... medical tests in clinical and anatomic pathology. ... Laboratories' clients include more than half of ...
... ARUP Laboratories is a national reference laboratory ... research and development. ARUP offers an extensive ... medical tests in clinical and anatomic pathology. ... Laboratories' clients include more than half of ...
Medicine Products: