Navigation Links
Engineers use blood's hydrodynamics to manipulate stem, cancer cells
Date:1/23/2008

A tiny, implantable device has pulled adult stem cells out of a living rat with a far greater purity than any present technique.

The test of the device designed by Michael R. King, associate professor of biomedical engineering at the University of Rochester, will be reported in the March 3 issue of the British Journal of Haematology.

Its the kind of research that, before we tried it, we never would have expected such a remarkable result straight out of the gate, says King. Were finding we can play off the hydrodynamics of moving blood to isolate and manipulate specific cell populations with great efficiency.

King is at the forefront of a new field; manipulating stem cells, white blood cells, and even cancer cells by exploiting the mechanics of the cells movement with such precision that he is having success capturing and even reprogramming several cell types as they pass through the device, he says.

A chance encounter between an engineer and a hematology clinician gave rise to the field in 2002.

King was studying how certain white blood cells, called neutrophils, know how to migrate to a point of infection. He observed how, near an injury site, the walls of the nearby blood vessels expressed an adhesive protein called a selectin, and if passing neutrophils brushed against these selectins, they stuck to the vessel wall.

But the cells did not remain stuckthey rolled. With a precise balance between the adhesion of the selectins and the forces of the flowing bloodstream, the cells could move much more slowly as they approached the infection site. With that slowed pace, the cell can look for a second signal on the vessel wall that tells the cell to exit the vessel by squeezing between cells in the wall and moving directly to the site of infection.

One reason the system is so effective is that only the neutrophils respond to those selectins, so only neutrophils slow down in the blood.

King was working out the physical dynamics of this neutrophil rolling in his office one day when Jane Liesveld, a hematology clinician doing work on bone marrow stem cells at the University of Rochester, walked by and noticed a poster of Kings work in the hallway outside his office.

She dropped in and said, I have a pretty plentiful source of primary stem cells from patients. Can you think of any biophysical research to do with that" says King. The stem cell angle just fell from the sky.

As King worked with Liesveld he found that the basic rolling mechanism was the foundation of a number of other processes, including stem cell transplantationa natural phenomenon where stem cells move in and out of bone tissue via the blood. In 2004, he found that he could coat a material with specific adhesive selectins and capture living stem cells. This collaboration resulted in two human stem cell papers published just within the last month: in Biotechnology Progress (Charles et al., 2007) and Clinical Chemistry (Narasipura et al., 2007).

In the new British Journal of Haematology paper, King and colleagues show they can take the process a step further by implanting the device in a living rat with the selectin coating remaining active for 1-2 hours. When the tube was removed, King found hed indeed captured cells straight out of the bloodstream, including contaminantsnon-stem cellsas expected. What he didnt expect was how many of the cells were viable stem cells.

I was astounded, says King. More than 25 percent of the sample was stem cells. Its amazing because even when you use drugs to increase the number of free stem cells in the blood, they still only make up less than 1 percent of all cells. If you use traditional methods to collect stem cells, centrifuging the rats blood, even in these drug-treated rats you might get 3 or 4 percent stem cellsmeaning only 3 or 4 percent of the cells you obtain are stem cells.

King points out that centrifugal methods currently produce an overall higher stem cell yield because they start with far more blood material, but he believes his microscale device can be scaled up to significantly larger capacity.

King is even more enthusiastic about his work in reprogramming cells that pass through his device. As the cell rolls across the adhesive surface, it can be forced to contact other proteins on the surface. King says these proteins can be designed to steer a stem cells development, forcing it to become a specific type of blood, bone, or muscle cell.

King hopes someday an implantable device could continuously reprogram errant neutrophils, but he is already hard at work on a device that holds the same promise for cancerous cells.

Cancer cells use the same rolling mechanism to travel around the body and lodge in interstitial tissue, so King has already focused on isolating the selectins that cancer cells respond to. His lab is working to create a microscale tube that might attract cancer cells and use permanent receptor-mediated triggering proteins to reprogram them to self-destruct. With his microscale tube device, King has already verified that he can control the rolling adhesion of various types of cancer cells, including leukemias, prostate, retinoblastoma, and colorectal cancer cells.

One of our ultimate goals is to develop an implantable device that will selectively remove metastatic cells from the blood, says King. Those cells can predate detectable tumors by years, so we might catch them before they become dangerous.


'/>"/>

Contact: Jonathan Sherwood
jonathan.sherwood@rochester.edu
585-273-4726
University of Rochester
Source:Eurekalert

Related medicine news :

1. Engineers developing new cements to heal spinal fractures
2. SMRT Architects and Engineers Selected to Design New $115M Digital X-Ray Manufacturing Facility for GE Healthcare
3. Microchip-based device can detect rare tumor cells in bloodstream
4. Microchip Spots Stray Tumor Cells in the Bloodstream
5. CoolTouch Introduces CoolLipo(TM) Laser System, Recently FDA Cleared for Laser-Assisted Lipolysis
6. Conventional prognostic factors fail to explain better prostate cancer survival in most Asian men
7. Survival differences by race most apparent in advanced stages of breast cancer
8. MRI finds breast cancer before it becomes dangerous
9. Investigators uncover intriguing clues to why persistent acid reflux sometimes turns into cancer
10. Pathway links inflammation, angiogenesis and breast cancer
11. Radiologists encouraged to look beyond cancer for clinically unseen diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... June 25, 2016 , ... "With 30 hand-drawn hand ... project," said Christina Austin - CEO of Pixel Film Studios. , ProHand Cartoon’s ... within Final Cut Pro X . Simply select a ProHand generator and drag ...
(Date:6/25/2016)... ... 25, 2016 , ... Dr. Calvin Johnson has dedicated his ... implemented orthobiologic procedures as a method for treating his patients. The procedure is ... to perform the treatment. Orthobiologics are substances that orthopaedic surgeons use to help ...
(Date:6/24/2016)... Angeles, CA (PRWEB) , ... June 24, 2016 , ... ... surgery procedures that most people are unfamiliar with. The article goes on to state ... procedures, but also many of these less common operations such as calf and cheek ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Marcy was in a crisis. Her son ... lash out at his family verbally and physically. , “When something upset him, he couldn’t ... would use it. He would throw rocks at my other children and say he was ...
(Date:6/24/2016)... ... June 24, 2016 , ... ... for accelerated orthodontic treatment. Dr. Cheng has extensive experience with all areas of ... AcceleDent, and accelerated osteogenic orthodontics. , Micro-osteoperforation is a revolutionary adjunct to ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... LEXINGTON, Mass. , June 24, 2016   ... specialty pharmaceutical company developing innovative inhaled drugs, announced today ... when Russell Investments reconstituted its comprehensive set ... 2016. "This is an important milestone for ... "It will increase shareholder awareness of our progress in ...
(Date:6/23/2016)... , June 23, 2016  MedSource announced ... as its e-clinical software solution of choice.  This ... best possible value to their clients by offering ... The preferred relationship establishes nowEDC as the EDC ... for MedSource,s full-service clients.  "nowEDC has long been ...
(Date:6/23/2016)... 2016 Research and Markets has ... by Type (Organic Chemical (Sugar, Petrochemical, Glycerin), Inorganic Chemical), ... Parenteral) - Global Forecast to 2021" report to ... The global pharmaceutical excipients market is projected to reach ... 6.1% in the forecast period 2016 to 2021. ...
Breaking Medicine Technology: