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Embryonic stem cells thrive when shaken
Date:9/10/2007

esearchers experimented with the shaking plate and determined that they could consistently produce samples with healthier, more uniform cells just by gently sloshing the dishes of stem cells on a shaker plate. The method proved to be much simpler and more space efficient than the current standard for producing embryonic stem cells, McDevitt said.

We can throw many cells in a dish and not have to worry about clumping and cell survival, McDevitt said. We call it the set it and forget it method for growing stem cells.

While the secret to the shaken stem cells success is still unclear, its suspected that the movement of the fluid likely increases nutrient distribution to cells, creating healthier cells, McDevitt said.

The Georgia Tech and Emory research team then began experiments to determine if the motion could be used to control the size of the cell aggregates and type of cells the embryonic cells would eventually become and found that there was a correlation between different types and speeds of movement and the phenotype and size of the stem cells.

Much work remains to be done before the movement control concept could be used to influence what types of cells embryonic stem cells eventually become. The teams next goal is to pinpoint more precisely exactly what speeds and manners of shaking can influence stem cell phenotype.


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Contact: Megan McRainey
megan.mcrainey@icpa.gatech.edu
404-894-6016
Georgia Institute of Technology
Source:Eurekalert

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