Navigation Links
Embryonic stem cells thrive when shaken
Date:9/10/2007

ATLANTA (September 10, 2007) Embryos spend much of their time in the womb bobbing along with a mothers movement, and, surprisingly enough, new research from the Georgia Institute of Technology and Emory University suggests that embryonic stem cells may develop much better under similarly shaky conditions.

Georgia Tech and Emory researchers discovered that moderate and controlled physical movement of embryonic stem cells in fluid environments, similar to shaking that occurs in the womb, improves their development and suggests that different types of movement could some day be used to control what type of cell they become. The research was published in the September issue of the journal Stem Cells.

Embryonic stem cells develop under unique conditions in the womb, and no one has ever been able to study the effect that movement has on that development process, said Todd McDevitt, assistant professor in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University and head of the project. While labs typically add all sorts of things to their cultures to influence cell direction, we were able to control the levels of differentiation and size of cell clusters by simply providing some fluid motion.

It all started with a fortunate accident. Rich Carpenedo, a graduate student and first author of the paper, discovered by chance that a dish of embryonic stem cells left on a common lab shaker (typically used to slowly mix samples) had developed in greater numbers and more uniformly than cells grown in a static environment (i.e. unshaken).

Current popular methods of developing embryonic stem cells in the lab involve single droplets of cells separated by a great deal of space in the dish. This time - and space-consuming technique allows the cells to develop without excessive clumping (a frequent problem for stem cells developed in the lab) and for a greater number to survive in a small space.

Researchers experimented with the shaking plate and determined that they could consistently produce samples with healthier, more uniform cells just by gently sloshing the dishes of stem cells on a shaker plate. The method proved to be much simpler and more space efficient than the current standard for producing embryonic stem cells, McDevitt said.

We can throw many cells in a dish and not have to worry about clumping and cell survival, McDevitt said. We call it the set it and forget it method for growing stem cells.

While the secret to the shaken stem cells success is still unclear, its suspected that the movement of the fluid likely increases nutrient distribution to cells, creating healthier cells, McDevitt said.

The Georgia Tech and Emory research team then began experiments to determine if the motion could be used to control the size of the cell aggregates and type of cells the embryonic cells would eventually become and found that there was a correlation between different types and speeds of movement and the phenotype and size of the stem cells.

Much work remains to be done before the movement control concept could be used to influence what types of cells embryonic stem cells eventually become. The teams next goal is to pinpoint more precisely exactly what speeds and manners of shaking can influence stem cell phenotype.


'/>"/>

Contact: Megan McRainey
megan.mcrainey@icpa.gatech.edu
404-894-6016
Georgia Institute of Technology
Source:Eurekalert

Related medicine news :

1. Embryonic Cells Might Prevent Parkinson’s Disease From Worsenin
2. Embryonic Genetic Testing Is Boosting IVF Pregnancies
3. Sperm stem cells could be an alternative to embryonic stem cells in future
4. T-cells Developed from Human Embryonic Stem Cells
5. Treatment of Spinal Cord Injury with Embryonic Stem Cells
6. Human Embryonic Stem Cells Could Be Later Used In Therapeutic Cloning
7. Researchers Create Genetically-Matched Embryonic Stem Cells for Transplantation
8. Embryonic Selection of Sex can Avoid Conceiving Blind Children
9. Lipid Plays Big Role in Embryonic Development
10. Mice can Produce Embryonic Stem Cells Similar to That of Humans
11. Bush Again Vetoes Embryonic Stem Cell Research Bill
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/27/2016)... ... May 27, 2016 , ... Two director-level employees of ... Tribute to Women and Industry (TWIN) 2016 honorees. The award recognizes businesswomen who ... this year, Geri Boone, Director of the MLTSS (Managed Long-Term Services and Supports) Program ...
(Date:5/27/2016)... ... 27, 2016 , ... This campaign aims to provide a path to improved ... can control and change. , As nearly 795,000 Americans suffering from a new or ... States. Plus, with an estimated 129,000 of these people dying from stroke, it’s become ...
(Date:5/27/2016)... ... May 27, 2016 , ... Aimed at nurses ... human interest stories, courtesy of leaders in the nursing and health care industry. ... from leading advocates and associations—namely Jones & Bartlett Learning. , Jones & Bartlett ...
(Date:5/26/2016)... (PRWEB) , ... May 26, 2016 , ... Despite last ... meeting, expect Janet Yellen and company to wait until March 2017 for an interest ... University’s J. Mack Robinson College of Business. , “The Federal Open Market Committee (FOMC) ...
(Date:5/26/2016)... ... May 26, 2016 , ... ... hosted members and suppliers for its inaugural Member Conference at the Paris Hotel ... of elevating the operational health of America’s healthcare providers. , The conference was ...
Breaking Medicine News(10 mins):
(Date:5/26/2016)... A key trend that will ... of new treatments. Cardax, a development stage life sciences ... therapy is expected to fulfil large unmet medical needs ... studies to develop new treatments for osteoarthritis. One such ... in osteoarthritis are being investigated, and early trials of ...
(Date:5/25/2016)... FDA 510(k) clearance covers Confocal Miniprobes ... and surgical applications Mauna Kea Technologies ... multidisciplinary confocal laser endomicroscopy (CLE) platform, today announced ... the 12 th 510(k) clearance from United ... FDA clearance covers Confocal Miniprobes indicated for use ...
(Date:5/25/2016)...  Zymo Research Corp. announced today the final ... that help researchers obtain the most accurate and ... rapid growth of the study of microbiomes has ... methods to improve the reproducibility and quality of ... every step of the measurement process including collection ...
Breaking Medicine Technology: