Navigation Links
Embryonic stem cells thrive when shaken
Date:9/10/2007

ATLANTA (September 10, 2007) Embryos spend much of their time in the womb bobbing along with a mothers movement, and, surprisingly enough, new research from the Georgia Institute of Technology and Emory University suggests that embryonic stem cells may develop much better under similarly shaky conditions.

Georgia Tech and Emory researchers discovered that moderate and controlled physical movement of embryonic stem cells in fluid environments, similar to shaking that occurs in the womb, improves their development and suggests that different types of movement could some day be used to control what type of cell they become. The research was published in the September issue of the journal Stem Cells.

Embryonic stem cells develop under unique conditions in the womb, and no one has ever been able to study the effect that movement has on that development process, said Todd McDevitt, assistant professor in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University and head of the project. While labs typically add all sorts of things to their cultures to influence cell direction, we were able to control the levels of differentiation and size of cell clusters by simply providing some fluid motion.

It all started with a fortunate accident. Rich Carpenedo, a graduate student and first author of the paper, discovered by chance that a dish of embryonic stem cells left on a common lab shaker (typically used to slowly mix samples) had developed in greater numbers and more uniformly than cells grown in a static environment (i.e. unshaken).

Current popular methods of developing embryonic stem cells in the lab involve single droplets of cells separated by a great deal of space in the dish. This time - and space-consuming technique allows the cells to develop without excessive clumping (a frequent problem for stem cells developed in the lab) and for a greater number to survive in a small space.

Researchers experimented with the shaking plate and determined that they could consistently produce samples with healthier, more uniform cells just by gently sloshing the dishes of stem cells on a shaker plate. The method proved to be much simpler and more space efficient than the current standard for producing embryonic stem cells, McDevitt said.

We can throw many cells in a dish and not have to worry about clumping and cell survival, McDevitt said. We call it the set it and forget it method for growing stem cells.

While the secret to the shaken stem cells success is still unclear, its suspected that the movement of the fluid likely increases nutrient distribution to cells, creating healthier cells, McDevitt said.

The Georgia Tech and Emory research team then began experiments to determine if the motion could be used to control the size of the cell aggregates and type of cells the embryonic cells would eventually become and found that there was a correlation between different types and speeds of movement and the phenotype and size of the stem cells.

Much work remains to be done before the movement control concept could be used to influence what types of cells embryonic stem cells eventually become. The teams next goal is to pinpoint more precisely exactly what speeds and manners of shaking can influence stem cell phenotype.


'/>"/>

Contact: Megan McRainey
megan.mcrainey@icpa.gatech.edu
404-894-6016
Georgia Institute of Technology
Source:Eurekalert

Related medicine news :

1. Embryonic Cells Might Prevent Parkinson’s Disease From Worsenin
2. Embryonic Genetic Testing Is Boosting IVF Pregnancies
3. Sperm stem cells could be an alternative to embryonic stem cells in future
4. T-cells Developed from Human Embryonic Stem Cells
5. Treatment of Spinal Cord Injury with Embryonic Stem Cells
6. Human Embryonic Stem Cells Could Be Later Used In Therapeutic Cloning
7. Researchers Create Genetically-Matched Embryonic Stem Cells for Transplantation
8. Embryonic Selection of Sex can Avoid Conceiving Blind Children
9. Lipid Plays Big Role in Embryonic Development
10. Mice can Produce Embryonic Stem Cells Similar to That of Humans
11. Bush Again Vetoes Embryonic Stem Cell Research Bill
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... ... Austin residents seeking Mohs surgery services, can now turn to Dr. Jessica Scruggs ... for medical and surgical dermatology. , Dr. Dorsey brings specialization to include Mohs surgery, ... Micrographic Surgery completed by Dr. Dorsey was under the direction of Glenn Goldstein, MD, ...
(Date:6/25/2016)... Oklahoma City, Oklahoma (PRWEB) , ... June 25, ... ... to helping both athletes and non-athletes recover from injury. Recently, he has implemented ... for the Oklahoma City area —Johnson is one of the first doctors to ...
(Date:6/24/2016)... ... , ... June 19, 2016 is World Sickle Cell Observance Day. In an ... of holistic treatments, Serenity Recovery Center of Marne, Michigan, has issued a ... Cell Disease (SCD) is a disorder of the red blood cells, which can cause ...
(Date:6/24/2016)... ... June 24, 2016 , ... Comfort Keepers® of San ... Society and the Road To Recovery® program to drive cancer patients to and from ... adults to ensure the highest quality of life and ongoing independence. Getting to ...
(Date:6/24/2016)... Vegas, Nevada (PRWEB) , ... June 24, 2016 ... ... Las Vegas client, The Grove Investment Group (TGIG), has initiated cultivation and processing ... Grove, in Las Vegas and Pahrump, Nevada. , Puradigm is the manufacturer of ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)...   Pulmatrix, Inc ., (NASDAQ: PULM ... announced today that it was added to the Russell ... comprehensive set of U.S. and global equity indexes on ... milestone for Pulmatrix," said Chief Executive Officer Robert ... progress in developing drugs for crucial unmet medical needs, ...
(Date:6/23/2016)... BEVERLY HILLS, Calif. , June 23, 2016 ... faced the many challenges of the current process. Many of ... option because of the technical difficulties and high laboratory costs ... would have to offer it at such a high cost ... to afford it. Dr. Parsa Zadeh , ...
(Date:6/23/2016)... 2016 Capricor Therapeutics, Inc. ... biotechnology company focused on the discovery, development and ... enrollment in its ongoing randomized HOPE-Duchenne clinical trial ... of its 24-patient target. Capricor expects the trial ... of 2016, and to report top line data ...
Breaking Medicine Technology: