Navigation Links
Drug therapy for PKU reverses heart damage
Date:5/13/2008

A pricy drug used to treat a rare but well-known genetic disorder may hold wider promise as a treatment for millions of Americans with potentially lethal enlarged hearts, due mainly to high blood pressure, a study from Johns Hopkins shows.

The common denominator in both phenylketonuria (PKU) and cardiac hypertrophy is the chemical tetrahydrobiopterin (BH4). In PKU, this enzyme coworker helps break down the molecule phenylalanine whose buildup is toxic to the brain. In the heart, BH4 helps build the chemical nitric oxide, which is needed for normal heart function and neutralizing toxic chemicals, called oxygen free radicals.

Doctors have used BH4 and diets that exclude phenylalanine for almost a decade to treat PKU, a so-called inborn error of metabolism that if left untreated causes irreversible brain damage. It affects an estimated 15,000 newborns in the United States each year.

Building on what has been known about BH4s activities, the Hopkins team, working with mice, found that treatment with BH4 stabilizes the pumping function of failing, enlarged hearts and dramatically shrinks the muscle size in a relatively short timeframe of just over a month. The teams findings appear in the May 20 edition of the journal Circulation.

Our results show for the first time the pivotal role played by BH4 in stopping and reversing the weakening and damage done even in severe cases to the heart muscle as a result of hypertension and subsequent hypertrophy, says study senior investigator David Kass, M.D., a professor at the Johns Hopkins University School of Medicine and its Heart Institute. This key evidence may help us develop new therapies that stop and reverse hypertrophy, preventing the disease from leading to end-stage heart failure and keeping affected individuals from needing heart-assist pumps or a treatment of last resort, the heart transplant.

Specifically, Kass and Belgian scientist, An Moens, M.D., a postdoctoral cardiology research fellow at Hopkins, fed a daily dose of 5 milligrams of BH4 per 25 grams of body weight to 31 mice whose hearts had been subjected to prolonged experimental hypertension created by constricting their aortas for four weeks.

Before and after treatment, heart function was monitored by several key tests, such as echocardiogram and magnetic resonance imaging, as well as catheters placed within the heart. This was supplemented by post-treatment tissue analysis. Results from this group were compared to another group of 25 mice, also subjected to high blood pressure, who were given placebo instead of BH4 during the same timeframe.

After induced heart failure and five weeks of subsequent therapy, researchers found that BH4-treated mice showed remarkable improvements, according to Kass, when compared to placebo-treated animals. Ejection fraction measures of heart pumping function not only stabilized with BH4, but improved, from an average 87 percent before heart failure to roughly 48 percent at the start of therapy, then back to 59 percent at the end of study. Meanwhile, average pumping function in placebo-treated mice showed a perilous decline, from 87 percent to 48 percent, to 35 percent.

Heart weight, as measured by muscle mass, showed similar results. Pressure stress resulted in mice hearts growing from an average 100 milligrams to 290 milligrams before therapy, returning to an average 209 milligrams in the BH4-treated hearts, while placebo-treated hearts grew increasingly worse, to an average 330 milligrams.

Improvements with BH4 therapy were almost as dramatic in at least three other measurements of organ health, including heart wall thickness, muscle cell size and fibrosis, and lowered chemical production of dangerous free radicals.

Hearts clearly got better from administering the drug, and our results offer proof of principle that damage to the left ventricle from hypertrophy can be stopped and reversed, providing a potential therapy for the lethal implications of prolonged high blood pressure, says Moens, now a cardiologist at the University of Antwerp in Belgium.

Kass expects clinical trials to start within a year.

Though no harmful side effects have been observed with BH4 therapy, Kass says, at present, the drug comes with a significant cost drawback. Technologically complex to manufacture, it is currently priced at $375 per 100 milligrams, and individual treatments for PKU have been estimated to cost as much as $33,000 per year. (The drug is currently sold as sapropterin dihydrochlorid, or Kuvan, by its manufacturer, BioMarin Pharmaceuticals.)

Researchers say that even before symptoms of heart failure emerge, such as chronic fatigue and shortness of breath, the heart muscle contracts more strongly to counteract high blood pressure. As muscle action increases, the heart grows and its walls becomes thicker, taking up space inside the hearts chambers normally reserved for blood. And despite harder muscle contractions, pumping function becomes increasingly weak as the heart can no longer push sufficient blood around the body to meet its energy needs.

According to the American Heart Association, more than 65 million American adults have high blood pressure, a major risk factor for developing larger-than-normal hearts. Experts say nearly a quarter of the adult population worldwide is estimated to have above- normal blood pressure. Hypertrophy increases by two to three times an adults risk of suffering cardiovascular disease, including heart failure and sudden cardiac death.

For nearly two decades, Kass and his team have studied the nitric oxide pathway for clues to its overall role in causing heart failure, in the belief that improving its function or lowering its production of oxygen free radicals could prevent or reverse the course of disease.

The Hopkins team says that BH4, whose tissue levels are degraded in stressed hearts from hypertrophy and the muscles weakened state, works by recoupling the enzyme nitric oxide synthase.

Researchers say this is the only form of the enzyme that functions normally, making more nitric oxide rather than free radicals. Testing of another potent antioxidant, Tempol, did not counter the effects of hypertrophy like BH4 and failed to recouple nitric oxide synthase. This tells us that BH4s targeted action is key to its benefits, says Kass.

Researchers next plan to look at combined therapies that could improve upon the effectiveness of BH4. Add-on drugs include vitamin C or folic acid, both of which are known to interact with the so-called enzyme cofactor, and researchers plan to monitor closely for the effects of combined therapy on heart function, size and weight.

In related work, Kass and his team have studied Viagra as a possible treatment for hypertrophy in its early stages. However, Kass points out that the Viagra-based research focuses on a different route, the phosphodiesterase-5 chemical pathway, and how it is involved in the process of heart failure. But, he says, BH4 is known to have some effect on this chemical pathway, so there could be some combination from these two therapies, which he also plans to study.


'/>"/>

Contact: David March
dmarch1@jhmi.edu
410-955-1534
Johns Hopkins Medical Institutions
Source:Eurekalert

Related medicine news :

1. Vision restoration therapy shown to improve brain activity in brain injured patients
2. HIV therapy in pregnancy-data support WHO recommendations
3. Family-based treatment more effective than supportive psychotherapy in treating bulimia
4. Trial to Test Gene Therapy for Angina in Women
5. One of the Largest Post-WHI Physician Surveys Shows More Education is Needed: Patient Misinformation About Hormone Therapy Remains High
6. Aromatherapy Gift Line Sheds Humorous Light on Modern Therapy
7. Chemotherapy may be culprit for fatigue in breast cancer survivors
8. Atlanta Falcons Physical Therapy Centers Set to Launch This Fall
9. New Drug No Substitute for Standard Blood-Clot Therapy
10. Stem Cell Therapy Disappoints Against Rare Kidney Ailment
11. Nucletron Announces Management Buy-Out to More Effectively Meet the Demands of Its Customers and the Radiation Therapy Community
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... TX (PRWEB) , ... June 25, 2016 , ... Austin ... of the American College of Mohs Surgery and to Dr. Russell Peckham for medical ... and highly effective treatment for skin cancer. The selective fellowship in Mohs Micrographic Surgery ...
(Date:6/25/2016)... , ... June 25, 2016 , ... ... and non-athletes recover from injury. Recently, he has implemented orthobiologic procedures as a ... area —Johnson is one of the first doctors to perform the treatment. Orthobiologics ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... June 19, ... the dangers associated with chronic pain and the benefits of holistic treatments, Serenity ... who are suffering with Sickle Cell Disease. , Sickle Cell Disease (SCD) is a ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Comfort Keepers® ... American Cancer Society and the Road To Recovery® program to drive cancer patients to ... and other adults to ensure the highest quality of life and ongoing independence. ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... ... client, The Grove Investment Group (TGIG), has initiated cultivation and processing operations at ... Las Vegas and Pahrump, Nevada. , Puradigm is the manufacturer of a complete ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... Research and Markets has announced the addition ... Chemical (Sugar, Petrochemical, Glycerin), Inorganic Chemical), Functionality (Filler, Binder, ... Forecast to 2021" report to their offering. ... excipients market is projected to reach USD 8.1 Billion ... forecast period 2016 to 2021. The ...
(Date:6/23/2016)... , June 23, 2016 Research and ... News Issue 52" report to their offering. ... influenza treatment creates a favourable commercial environment for MedImmune to ... patient base that will serve to drive considerable growth for ... would serve to cap sales considerably, but development is still ...
(Date:6/23/2016)... -- Revolutionary technology includes multi-speaker listening to ... leaders in advanced audiology and hearing aid technology, has ... the world,s first internet connected hearing aid that opens ...      (Photo: http://photos.prnewswire.com/prnh/20160622/382240 ) , ... firsts,: , TwinLink™ - the first dual ...
Breaking Medicine Technology: