Navigation Links
Drug discovery potential of natural microbial genomes
Date:1/22/2014

Scientists at the University of California, San Diego have developed a new genetic platform that allows efficient production of naturally occurring molecules, and have used it to produce a novel antibiotic compound. Their study, published this week in PNAS, may open new avenues for natural product discoveries and drug development.

According to lead investigator Bradley S. Moore, PhD, of the Scripps Institution of Oceanography and Skaggs School of Pharmacy and Pharmaceutical Sciences at UC San Diego, the findings demonstrate a "plug and play" technique to trigger previously unknown biosynthetic pathways and identify natural product drug candidates.

"In my opinion, the new synthetic biology technology we developed which resulted in the discovery of a new antibiotic from a marine bacterium is just the tip of the iceberg in terms of our ability to modernize the natural product drug discovery platform," Moore said.

The ocean, covering 70 percent of the earth's surface, is a rich source of new microbial diversity for the discovery of new natural products effective as drugs for treating infections, cancer and other important medical conditions. Most natural antibiotics are complex molecules that are assembled by a special group of enzymes genetically encoded in the microbe's chromosome.

But it often proves difficult to grow the newly discovered ocean bacteria in the laboratory, or to get them to produce their full repertoire of natural products.

The UC San Diego scientists harvested a set of genes predicted to encode a natural product from ocean bacteria, then used the synthetic biology technology to identify and test a totally new antibiotic taromycin A found to be effective in fighting drug-resistant MRSA.

"Antibiotic resistance is critical challenge to the public health. Most antibiotics, such as penicillin, used in human medicine are natural molecules originally isolated from microbes in the soil or rainforest part of the chemical warfare that microbes deploy to out-compete one another and secure their niche in the environment," said co-investigator Victor Nizet, MD, professor of pediatrics and pharmacy at UC San Diego.

Such microbes have the genetic capacity to biosynthesize a wide range of specialized compounds. Although next-generation sequencing technologies can potentially exploit this capacity as an approach to natural drug discovery, researchers currently lack procedures to efficiently link genes with specific molecules. To help bridge this gap, the UC San Diego researchers developed a genetic platform based on transformation-associated recombination (TAR) cloning, which efficiently produces natural product molecules from uncharacterized gene collections.

The researchers applied the platform to yeast, targeting the taromycin gene cluster because of its high degree of similarity to the biosynthesis pathway of daptomycin, a clinically approved antibiotic used to treat infections caused by multi-resistant bacteria. "The technique has the potential to unlock the drug discovery potential of countless new and mysterious microbes," Nizet concluded.


'/>"/>

Contact: Debra Kain
ddkain@ucsd.edu
619-543-6163
University of California - San Diego
Source:Eurekalert

Related medicine news :

1. Scientist awarded $1 million grant to develop tools for hepatitis C treatment discovery
2. Washingtons Life Sciences Discovery Fund awards commercialization grants
3. Discovery could help to develop drugs for organ transplant and cancer patients
4. Feelings of immaturity accompany alcohol misuse into adulthood; discovery could improve treatments
5. H1N1 discovery paves way for universal flu vaccine: UBC research
6. Scientists make breakthrough in bile duct cancer with discovery of new gene mutations
7. Researchers make promising discovery in pursuit of effective lymphoma treatments
8. Discovery suggests new combination therapy strategy for basal-like breast cancers
9. Discovery of Gene May Lead to New Male Contraceptive
10. 5 more pharmaceutical companies join NIH initiative to speed therapeutic discovery
11. Illnesses in Colorado childrens hospital prompts discovery of contaminated alcohol pads
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/28/2017)... ... June 28, 2017 , ... Eating disorders have the highest mortality rate of ... one person dies as a direct result from an eating disorder in the U.S. ... providers. The iaedp Foundation meets this challenge by offering what has become the leading ...
(Date:6/28/2017)... ... June 28, 2017 , ... Park Systems , ... Luncheon for all SEMICONWest attendees and Park customers on July 11, 2017 ... from Dr. Sang-il Park, Chairman & CEO of Park Systems, and Prof. Krishna ...
(Date:6/28/2017)... (PRWEB) , ... June 28, 2017 , ... ... assistance and financial consultations to communities in northern Virginia and DC, is announcing ... help provide for patients with Alzheimer’s and other disorders that lead to memory ...
(Date:6/27/2017)... PA and London UK (PRWEB) , ... June 27, 2017 , ... ... applying to a clinical study is whether they can trust the sponsor to pay ... it is vital that sponsors and CROs establish payment strategies that encourage sites to ...
(Date:6/27/2017)... ... 27, 2017 , ... Building on the success of the ... sixth state to pass legislation which ensures that children can possess and use ... the states of Alabama, Arizona, Florida, Utah and Washington who have also approved ...
Breaking Medicine News(10 mins):
(Date:6/13/2017)... Ind. , June 13, 2017 Zimmer Biomet ... musculoskeletal healthcare, today announced that the U.S. Food and Drug ... dated June 3, 2015 relating to its Zhejiang, ... "The successful clearance of the ... manufacturing facility is a measure of the progress ...
(Date:6/10/2017)... June 9, 2017  Shane K. Burchfield, DPM, is recognized by ... as a Podiatrist in Alabama . ... First Foot Care. He brings over 20 years of experience, as ... and healthcare, to his role. ... PC is pleased to welcome you to his practice," ...
(Date:6/8/2017)... 8, 2017  Less than a month ago, amateur ... 200,000 companies, including hospital networks, in over 150 countries. ... of the largest online extortion attempts ever recorded. With ... it is imperative that providers understand where the risks ... this — and many other very real cyber threats.  ...
Breaking Medicine Technology: