Navigation Links
Double whammy: RNAi enhances lung cancer therapy
Date:3/20/2012

Non-small cell lung cancer (NSCLC), the most common form of lung cancer, is usually treated with surgery and chemotherapy. However, a small group of patients can also be helped by treatment with tyrosine kinase inhibitors (TKI). New research published in BioMed Central's open access journal BMC Medicine, shows that blocking production of epidermal growth factor receptor (EGFR) using RNAi, alongside TKI (or antibody therapy), could enhance the effect of TKI on NSCLC cell death, and slow cell growth.

There are 1.2 million new cases of lung cancer worldwide every year and about 75-85% of these are NSCLC. 10% of cases with NSCLC have a mutation in the gene responsible for making EGFR, and three quarters of these patients respond well to TKIs. However others are unresponsive, and, even for the people who responded at first, the cancer always ultimately becomes resistant to TKI therapy.

In an attempt to circumvent this problem researchers from Universitair Ziekenhuis Brussel, in Belgium, tested the effect of adding RNAi to standard TKI or antibody therapy in NSCLC cells. Proteins are made in the cell's cytoplasm, but DNA is stuck inside the nucleus. Consequently, in order to produce a protein, a cell must first copy the gene's DNA sequence, producing an intermediary molecule called RNA which is able to travel out of the nucleus to the cytoplasm. RNAi 'interferes' with the mechanism for producing proteins by blocking proper function of the intermediary RNA stage.

A team led by Prof De Grve and his co-worker, Gang Chen, found that the small interfering RNA (siRNA) molecule they designed was able to inhibit EGF protein production in all the NSCLC cell lines tested. It was also able to slow down cell growth and increase cell death (apoptosis) in all the cell lines although some responded better than others. When they tested siRNA alongside TKI and or monoclonal antibodies they discovered that the siRNA had an additional positive effect on the cell lines than the standard treatment alone.

Prof De Grve explained, "Some EGFR mutations were more sensitive to siRNA than others - we saw especially good results against the mutation in exon 19. We think that this reflects the fact that the growth of cells with this mutation is driven by EGFR, but that other mutations are not as active. A different EGFR mutation produces cells which cannot be inhibited by TKI, however even these cells were sensitive to siRNA."

These results provide hope for NSCLC patients with EGFR mutations, as it could potentially enhance TKI therapy. It may also help those who do not respond to TKI, or who have become resistant to TKI therapy. Additionally this treatment may benefit another group of patients where the gene for EGFR is too active in their cancer and too much EGFR is produced.


'/>"/>

Contact: Dr. Hilary Glover
hilary.glover@biomedcentral.com
44-203-192-2370
BioMed Central
Source:Eurekalert

Related medicine news :

1. U.S. Deaths From Gastro Infections Doubled Over 8 Years: CDC
2. New drug, Vemurafenib, doubles survival of metastatic melanoma patients
3. New street drug bath salts packs double punch
4. New combo of chemo and well-known malaria drug delivers double punch to tumors
5. Pot Use Could Double Risk of Car Crash, Research Shows
6. Rate of physician referrals nearly doubled
7. Targeting EETs to treat cardiovascular disease may prove a double-edged sword
8. Scientists may be able to double efficacy of radiation therapy
9. Asthma rates double for WTC first responders
10. Use of opioid painkillers for abdominal pain has more than doubled
11. STD Complication May Double Infertility Risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/1/2017)... York, NY & Greenwich, CT (PRWEB) , ... March 01, 2017 ... ... many runners, especially in the winter months when people don’t want to stop training ... the knees – and setting the treadmill to a small incline can help protect ...
(Date:3/1/2017)... ... , ... “McFarnia”: a suspenseful tale of a father who vanishes without a ... disappearance. “McFarnia” is the creation of published author Tony Pierzchala, a former Army man, ... a Chaplain with Transport for Christ at a truck stop in the Greensboro area. ...
(Date:3/1/2017)... ... ... of Love”: a tale of young love and war. ““Letter of Love”” is the ... Salvador, wife, mother, and co-founder of a Christian College. , “I turned to ... poor communities, and I study psychology. I lived the civil war. ” --Maria Teresa ...
(Date:3/1/2017)... ... , ... “The Affair is Over Now Go Live Your Life Abundantly”: a ... affair. “The Affair is Over Now Go Live Your Life Abundantly” is the creation ... dedicated her professional life to teaching and writing about marriage and family life in ...
(Date:2/28/2017)... ... , ... Answering strong demand for its medical device software design and development ... in the Soma neighborhood in San Francisco on March 1, 2017. The opening also ... systems architecture subject matter experts. , Orthogonal serves a sizeable clientele in the Bay ...
Breaking Medicine News(10 mins):
(Date:3/1/2017)... DIEGO , March 1, 2017  Aethlon ... European Patent Office has granted European Patent Number ... In recent years, exosomes have emerged ... a wide range of disease conditions, including cancer, ... (CTE).  This granted European Patent is an important ...
(Date:3/1/2017)... 1, 2017  Numotion, the nation,s leading provider of ... Tek RMD ("robotic mobilization device") by Matia Robotics in ... is a motorized standing movement device that offers the ... to complete everyday activities from a standing position. Unlike ... RMD unassisted. Numotion is the exclusive distributor of the ...
(Date:3/1/2017)... ... and Markets has announced the addition of the "Global Infertility ... The Global Infertility Therapy Partnering Terms and Agreements since 2010 ... into by the world,s leading healthcare companies. The report ... discovery or development stage whereby the licensee obtains a right or ...
Breaking Medicine Technology: