Navigation Links
Does HBV infection induce acute cellular DNA damage?
Date:11/11/2008

Eukaryotic cells employ multiple strategies of checkpoint signaling and DNA repair mechanisms to monitor and repair damaged DNA. There are two branches of the checkpoint response pathway, ataxia telangiectasia-mutated (ATM) pathway and ATM-Rad3-related (ATR) pathway. Virus replication presents the host cells with large amounts of exogenous genetic material, including DNA ends and unusual structures. Therefore, infected cells recognize viral replication as a DNA damage stress and elicit DNA damage signal transduction, which ultimately induces apoptosis as part of host immune surveillance. There was no evidence so far that the ATM/ATR kinases or their downstream pathways are triggered by HBV infection.

A research article to be published on October 28, 2008 in the World Journal of Gastroenterology addresses this question. This research group was leaded by Dr Hui Zhong from Beijing Institute of Biotechnology, China.

Using HL7702 hepatocytes with HBV-positive serum as material, they evaluate protein expression levels in HBV infected cells or in non-infected cells; immunofluorescence to show ATR foci ands Chk1 phosphorylation foci formation; flow cytometry to analyze the cell cycle and apoptosis; ultraviolet radiation (UV) and ionizing radiation (IR) treated cells to mimic DNA damage; Trypan blue staining to count the viable cells.

They found that HBV infection induced increased steady state of ATR protein and increased phosphorylation of multiple downstream targets including Chk1, p53 and H2AX. In contrast to ATR and its target, the phosphorylated form of ATM at Ser-1981 and its downstream substrate Chk2 phosphorylation at Thr-68 did not visibly increase upon infection. However, the level of Mre11 and p21 were reduced beginning at 0.5 h after HBV-positive serum addition. Also, HBV infection led to transient cell cycle arrest in the S and the G2 phases without accompanying increased apoptosis. Research on analyzing cell survival ch
'/>"/>

Contact: Lai-Fu Li
wjg@wjgnet.com
0086-105-908-0039
World Journal of Gastroenterology
Source:Eurekalert

Page: 1 2

Related medicine news :

1. Battling Bacteria in the Blood: U-M Researchers Tackle Deadly Infections
2. Intestinal Infection Afflicts 13 of 1,000 Hospital Patients, Infection Rates 6.5 - 20 Times Greater Than Previous Estimates, New Study Says
3. Activity Against MRSA is the Most Important Attribute Influencing Physicians Antibiotic Selection for the Treatment of Skin and Skin Structure Infections
4. NIAID media availability: Seizures following parasitic infection associated with brain swelling
5. MRI can eliminate unnecessary surgery for children with suspected musculosketal infections
6. Persistent bacterial infection exploits killing machinery of immune cells
7. Special Mouth Rinse Spots, Tracks Human Papillomavirus Infection
8. Professional Sports Teams Should Use MRSA Screening and Other Aggressive Methods to Prevent Staph Infections
9. Older Blood Boosts Chances of Infection in Transfusion Patients
10. Old Blood Linked To Infection
11. Genetic Approach Boosts Yeast Infection Treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/24/2014)... 25, 2014 A few weeks have ... the incredible high jump by Inika McPherson is a ... June, the USATF National Championships hosted the best track ... Inika McPherson, won US Nationals with the 2nd best ... Record for the biggest height over head jump in ...
(Date:7/24/2014)... (PRWEB) July 24, 2014 The ... online training course designed to help users generate traffic ... generating a buzz of excitement throughout the internet marketing ... the attention of HonestyFirstReviews.com’s Tiffany Hendricks prompting a comprehensive ... who’s been involved in internet marketing will tell you, ...
(Date:7/24/2014)... The report, “Content Delivery Networks (CDN) ... various sub-segments with an in-depth analysis and forecasting ... restraints for this market with insights on trends, ... tables and 36 figures spread through 157 pages ... Market” at http://www.marketsandmarkets.com/Market-Reports/content-delivery-networks-cdn-market-657.html . , Early ...
(Date:7/24/2014)... In a July 9th article in the Money ... Lotus Blossom Consulting LLC , was quoted in a Family ... Cut the High Cost of Infertility . Her advice began ... maximize your chance of success from the first attempt.” The ... “I am always happy to talk with the media ...
(Date:7/24/2014)... HealthDay Reporter THURSDAY, July ... don,t view their kids as unhealthy or recognize the health ... study. The children of the families surveyed for the ... Children,s Hospital in Providence, R.I. "A third categorized their ... Dr. Kyung Rhee, now an assistant professor of pediatrics at ...
Breaking Medicine News(10 mins):Health News:Setting A New High Jump World Record In Zensah® Compression 2Health News:Setting A New High Jump World Record In Zensah® Compression 3Health News:Traffic And Leads Training Academy - Review Examining Jeff Johnson’s New IM Course Released 2Health News:CDN Market by Delivery Technologies & Solutions Expected to Reach $12.16 Billion by 2019 - New Report by MarketsandMarkets 2Health News:CDN Market by Delivery Technologies & Solutions Expected to Reach $12.16 Billion by 2019 - New Report by MarketsandMarkets 3Health News:CDN Market by Delivery Technologies & Solutions Expected to Reach $12.16 Billion by 2019 - New Report by MarketsandMarkets 4Health News:Mindy Berkson, Infertility Expert and Founder, Lotus Blossom Consulting LLC: Leading the Pathway to Parenthood 2Health News:Mindy Berkson, Infertility Expert and Founder, Lotus Blossom Consulting LLC: Leading the Pathway to Parenthood 3Health News:Parents of Obese Kids Often View Them as Healthy 2Health News:Parents of Obese Kids Often View Them as Healthy 3
... (Sept. 20, 2007) A Jekyll-Hyde mechanism that both ... the basis for new cancer-fighting drugs. , Scientists in ... that a certain transcription factora protein that binds to ... genes on and offknown to aid in handling stresses ...
... scientist at Leeds whose research is challenging conventional thinking ... has secured funding to further investigate her findings. , ... Sciences has been awarded 80,000 from Heart Research UK ... affecting the heart muscle cell. , For ...
... the agency is doing , , THURSDAY, Sept. 20 ... prescription medicines for health, many are confused and distrustful ... new survey finds. , In fact, many of those ... and Drug Administration approves drugs, and almost half judged ...
... Developments in Solid Organ Transplantation, DALLAS, ... Review",is the focus of a one-day meeting ... Grapevine, Texas. Co-sponsored by the American Society ... Professionals,(NATCO), the conference agenda contains information about ...
... Increase ... SAN ANTONIO, Sept. 20 AT&T Inc. (NYSE: ... portfolio of radio frequency,identification (RFID) tracking offers for health ... operations of hospitals and other,health care facilities. AT&T ...
... MDA stunning discovery by German scientists may make blood ... as washing your hair. In the October print issue ... that the estimated 100,000 hair follicles on each persons ... EPO, the hormone primarily responsible for the creation of ...
Cached Medicine News:Health News:Heat shock proteins are co-opted for cancer 2Health News:Research to shed new light on how statins benefit heart patients 2Health News:Americans Confused About FDA and Drug Safety 2Health News:Americans Confused About FDA and Drug Safety 3Health News:Americans Confused About FDA and Drug Safety 4Health News:Dallas-Area Transplant Meeting Puts Spotlight on Key Issues 2Health News:AT&T Offers 'Network Industry-First' RFID Solution for Health Care Organizations 2Health News:AT&T Offers 'Network Industry-First' RFID Solution for Health Care Organizations 3Health News: New discovery leaves blood-doping athletes scratching their heads 2
(Date:7/24/2014)... 24, 2014 Decision Resources Group finds that, ... countries ( France , Germany ... Spain and the United Kingdom ... first once-daily long-acting beta2 agonist/inhaled corticosteroid (LABA/ICS) fixed-dose combination ... Relvar as a first-line LABA/ICS FDC in the treatment ...
(Date:7/24/2014)... , July 24, 2014 /PRNewswire-iReach/ -- "North America Orthopedic ... market data on the North America Orthopedic Braces and ... US dollars, and volume (in units) and average price ... , Knee Braces and Supports , Ankle Braces ... , Upper Extremities Braces and Supports. ...
(Date:7/24/2014)... , July 24, 2014 Boston Children,s ... Tomorrow (#PedInno14), will feature an "Innovation Tank" – ... an opportunity to pitch their business or product solution ... funding and feedback on how to accelerate the innovation ... occurring the morning of Friday, October 31, will be ...
Breaking Medicine Technology:Surveyed EU5 Pulmonologists Expect that Relvar, the First Once-Daily LABA/ICS FDC, Will Experience Healthy Uptake in Asthma 2Surveyed EU5 Pulmonologists Expect that Relvar, the First Once-Daily LABA/ICS FDC, Will Experience Healthy Uptake in Asthma 3North America Orthopedic Braces and Supports Market (Knee, Ankle & Spinal Braces) to 2020 2North America Orthopedic Braces and Supports Market (Knee, Ankle & Spinal Braces) to 2020 3North America Orthopedic Braces and Supports Market (Knee, Ankle & Spinal Braces) to 2020 4North America Orthopedic Braces and Supports Market (Knee, Ankle & Spinal Braces) to 2020 5Taking on Tomorrow to Feature "Innovation Tank" Pitch Opportunity for Aspiring Pediatric Health Care Entrepreneurs 2Taking on Tomorrow to Feature "Innovation Tank" Pitch Opportunity for Aspiring Pediatric Health Care Entrepreneurs 3
... Zimmer Holdings, Inc. (NYSE: ZMH ; SIX: ZMH) ... of its senior unsecured notes in an underwritten public offering ... $500.0 million of 5.75% notes due 2039. The 10-year notes ... benchmark, and the 30-year notes were priced at 135 basis ...
... (Nasdaq: PCYC ) today announced that posters related ... at the 51st Annual Meeting of the American Society ... LA. , A material clinical update relating to ... programs will be presented at 6pm-8pm EST on Sunday, ...
Cached Medicine Technology:Zimmer Holdings, Inc. Agrees to Sell $1 Billion of Senior Notes 2Pharmacyclics Announces Poster Presentations and Clinical Update at the 51st Annual Meeting of the American Society of Hematology 2Pharmacyclics Announces Poster Presentations and Clinical Update at the 51st Annual Meeting of the American Society of Hematology 3Pharmacyclics Announces Poster Presentations and Clinical Update at the 51st Annual Meeting of the American Society of Hematology 4
... (PxE) is a licensed 96 well PCR machine, ... for money thermal cycling. It is built ... in manufacturing and retains a high level of ... products in the range. The combination of ...
Custom programming of 3 hot blocks and 1 cold block, 30% reduction in cycling time, Unmatched well-to-well uniformity, No gradient feature...
... With Exceptional Performance The sleek ... might be the first things ... is designed for performance. With ... premium, its small footprint is ...
... The 96-Well GeneAmp PCR System 9700 ... and Aluminum Sample Blocks Modules. The aluminum ... of PCR reactions and cycle sequencing in ... This allows the 96-well 9700 system to ...
Medicine Products: