The LOH analysis on sporadic carcinoma by means of microsatellite markers has become an effective way to find allelic deletion regions and then to find candidate tumor suppressor genes. In a previous study, it was found that D1S413 (1q31.1-32.1, 9.8cM) exhibited higher LOH frequencies, which indicated that the region might harbor the putative tumor suppressor gene(s). However, the allelic deletion region contained so many genes that it was inconvenient to continue the gene screening and functional study. Therefore, further LOH scanning with high-density microsatellite markers in the region was found to be necessary to narrow the research scope and select fewer candidate genes in the finite regions for functional research.
This study, performed by Dr. Chong-Zhi Zhou and his colleagues, is described in a research article to be published on March 14, 2008, in the World Journal of Gastroenterology.
They analyzed refined LOH mapping of 1q31.1-32.1 and found a minimal region of frequent deletion located within a 2-cM genomic segment at D1S413-D1S2622 on 1q31.3-32.1.
Then by searching in the databases, they presumed that CSRP1 might be the candidate colorectal carcinoma-related gene in this region.
In the view of the authors, the genetic changes in colorectal carcinogenesis are not fully understood. However, LOH analysis is an effective method to find informative loci and subsequently identify candidate tumor suppressor genes.
This study found the critical and precise deleted region on 1q31.1-32.1 and provided significant data to help reveal the mechanisms of colorectal carcinogenesis.
Further research should carry out microarray-based high-throughput gene screening and should include functional research for finding the potential tumor suppressor gene.
|Contact: Jing Zhu|
World Journal of Gastroenterology