Converting adult somatic cells to pluripotent stem cells using a si... (enerate iPS cells more efficiently -- 10...)

Converting adult somatic cells to pluripotent stem cells using a single virus

Date:1/7/2009

enerate iPS cells more efficiently -- 10 times higher than previously reported studies.

"The use of a single lentiviral vector for the derivation of iPS cells will help reduce the variability in efficiency that has been observed between different laboratories, thus enabling more consistent genetic and biochemical characterizations of iPS cells and the reprogramming process," the researchers concluded.

"We believe that the specific design of the cassette together with the fact that all four genes are expressed from the same transcript could account for the high efficiency we obtained" commented Cesar A. Sommer, first author in the paper and a postdoctoral fellow at Boston University Medical School's Gastroenterology Section.

Most importantly, several iPS clones were generated with a single viral integration, a major advance compared to the multiple integrations observed in other studies.

"Now we could move forward toward the elimination of the whole cassette using recombination technologies", noted Mostoslavsky.

Darrell N. Kotton, another co-author on the paper and an Assistant Professor at Boston University Medical School's Pulmonary Section mentioned that preliminary studies already confirmed that the STEMCCA vector works with high efficiency for the reprogramming of human cells.

'/>"/>

Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University
Source:Eurekalert

0

GOOD

Page: 1 2
Related medicine news :

1. New Lung Association Study Shows $142 Billion Benefit of Converting to Zero-Emission Vehicles by 2030
2. Popular Florida Restaurant Leads Hospitality Industry by Converting to Pasteurized Shell Eggs Usage
3. Adult-onset diabetes slows mental functioning in several ways, with deficits appearing early
4. Holiday Weight Gain May Contribute to Sleep Apnea Extra Pounds May Produce Severe Problems for Adults
5. Stem Cells Poised to Grow Organs for Transplant : Zannos Grekos, MD Presents Clinical Data of Research with Adult Stem Cells to Physicians at International Regenerative Biomedicine Conference
6. Potential autoimmunity-inducing cells found in healthy adults
7. Kimberly-Clark Introduces New Depend Underwear for Men & Women; Unveils Brands First Gender-Specific Adult Absorbent Underwear
8. Strategic video game improves critical cognitive skills in older adults
9. According to a New Government Survey, 38 Percent of Adults and 12 Percent of Children Use Complementary and Alternative Medicine
10. 38% of U.S. Adults Use Alternative Treatments
11. Half-Dose Flu Shot Works Well in Young Adults

Post Your Comments:

Converting adult somatic cells to pluripotent stem cells using a si...(enerate iPS cells more efficiently -- 10...),Health