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Converting adult somatic cells to pluripotent stem cells using a single virus
Date:1/7/2009

(Boston) -- A Boston University School of Medicine-led research team has discovered a more efficient way to create induced Pluripotent Stem (iPS) cells, derived from mouse fibroblasts, by using a single virus vector instead of multiple viruses in the reprogramming process. The result is a powerful laboratory tool and a significant step toward the application of embryonic stem cell-like cells for clinical purposes such as the regeneration of organs damaged by inherited or degenerative diseases, including emphysema, diabetes, inflammatory bowel disease, and Alzheimer's Disease.

Their research titled "iPS Cell Generation Using a Single Lentiviral Stem Cell Cassette" appears on line in the journal Stem Cells.

Prior research studies have required multiple retroviral vectors for reprogramming -- steps that depended on four different viruses to transfer genes into the cells' DNA essentially a separate virus for each reprogramming gene (Oct4. Klf4, Sox2 and cMyc). Upon activation these genes convert the cells from their adult, differentiated status to what amounts to an embryonic-like state.

However, the high number of genomic integrations -- 15 to 20 -- that typically occurs when multiple viruses are used for reprogramming, poses a safety risk in humans, as some of these genes (i.e. cMyc) can cause cancer. In addition, the viruses can integrate in cell locations turning on potential oncogenes.

The major milestone the six-member research team, led by Gustavo Mostoslavsky, Boston University Assistant Professor of Medicine in the Gastroenterology Section, achieved was combining the four vectors into a single "stem cell cassette" containing all four genes. The cassette (named STEMCCA) is comprised of a single multicistronic mRNA encoding the four transcription factors using a combination of 2A peptide technology and an internal ribosomal entry site (IRES).

With the STEMCCA vector, the researchers were able to g
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Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University
Source:Eurekalert

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