Navigation Links
Converting adult somatic cells to pluripotent stem cells using a single virus
Date:1/7/2009

(Boston) -- A Boston University School of Medicine-led research team has discovered a more efficient way to create induced Pluripotent Stem (iPS) cells, derived from mouse fibroblasts, by using a single virus vector instead of multiple viruses in the reprogramming process. The result is a powerful laboratory tool and a significant step toward the application of embryonic stem cell-like cells for clinical purposes such as the regeneration of organs damaged by inherited or degenerative diseases, including emphysema, diabetes, inflammatory bowel disease, and Alzheimer's Disease.

Their research titled "iPS Cell Generation Using a Single Lentiviral Stem Cell Cassette" appears on line in the journal Stem Cells.

Prior research studies have required multiple retroviral vectors for reprogramming -- steps that depended on four different viruses to transfer genes into the cells' DNA essentially a separate virus for each reprogramming gene (Oct4. Klf4, Sox2 and cMyc). Upon activation these genes convert the cells from their adult, differentiated status to what amounts to an embryonic-like state.

However, the high number of genomic integrations -- 15 to 20 -- that typically occurs when multiple viruses are used for reprogramming, poses a safety risk in humans, as some of these genes (i.e. cMyc) can cause cancer. In addition, the viruses can integrate in cell locations turning on potential oncogenes.

The major milestone the six-member research team, led by Gustavo Mostoslavsky, Boston University Assistant Professor of Medicine in the Gastroenterology Section, achieved was combining the four vectors into a single "stem cell cassette" containing all four genes. The cassette (named STEMCCA) is comprised of a single multicistronic mRNA encoding the four transcription factors using a combination of 2A peptide technology and an internal ribosomal entry site (IRES).

With the STEMCCA vector, the researchers were able to generate iPS cells more efficiently -- 10 times higher than previously reported studies.

"The use of a single lentiviral vector for the derivation of iPS cells will help reduce the variability in efficiency that has been observed between different laboratories, thus enabling more consistent genetic and biochemical characterizations of iPS cells and the reprogramming process," the researchers concluded.

"We believe that the specific design of the cassette together with the fact that all four genes are expressed from the same transcript could account for the high efficiency we obtained" commented Cesar A. Sommer, first author in the paper and a postdoctoral fellow at Boston University Medical School's Gastroenterology Section.

Most importantly, several iPS clones were generated with a single viral integration, a major advance compared to the multiple integrations observed in other studies.

"Now we could move forward toward the elimination of the whole cassette using recombination technologies", noted Mostoslavsky.

Darrell N. Kotton, another co-author on the paper and an Assistant Professor at Boston University Medical School's Pulmonary Section mentioned that preliminary studies already confirmed that the STEMCCA vector works with high efficiency for the reprogramming of human cells.


'/>"/>

Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University
Source:Eurekalert

Related medicine news :

1. New Lung Association Study Shows $142 Billion Benefit of Converting to Zero-Emission Vehicles by 2030
2. Popular Florida Restaurant Leads Hospitality Industry by Converting to Pasteurized Shell Eggs Usage
3. Adult-onset diabetes slows mental functioning in several ways, with deficits appearing early
4. Holiday Weight Gain May Contribute to Sleep Apnea Extra Pounds May Produce Severe Problems for Adults
5. Stem Cells Poised to Grow Organs for Transplant : Zannos Grekos, MD Presents Clinical Data of Research with Adult Stem Cells to Physicians at International Regenerative Biomedicine Conference
6. Potential autoimmunity-inducing cells found in healthy adults
7. Kimberly-Clark Introduces New Depend Underwear for Men & Women; Unveils Brands First Gender-Specific Adult Absorbent Underwear
8. Strategic video game improves critical cognitive skills in older adults
9. According to a New Government Survey, 38 Percent of Adults and 12 Percent of Children Use Complementary and Alternative Medicine
10. 38% of U.S. Adults Use Alternative Treatments
11. Half-Dose Flu Shot Works Well in Young Adults
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/23/2017)... ... 23, 2017 , ... Better education to coaches and parents ... work today at the American Orthopaedic Society for Sports Medicine’ s ... to prospectively document the association between sports specialization and lower extremity injuries in ...
(Date:7/23/2017)... ... July 23, 2017 , ... Technology ... safety and patient-provider relationships. New tools are helping patients become more actively involved ... Annual Health Care’s Most Wired® survey, released today by the American Hospital Association’s ...
(Date:7/23/2017)... Melbourne, Fla. (PRWEB) , ... July 23, 2017 ... ... Stern, M.D., has joined Florida Pain Relief Group – Melbourne, a practice owned ... to board certification in interventional pain management, Dr. Stern also is certified in ...
(Date:7/23/2017)... (PRWEB) , ... July 23, 2017 , ... ... to announce today the grant of US Patent No. 9,038,640 by the United ... RF technology and brings together the combination of Switching, Vacuum, and Cooling mechanisms ...
(Date:7/22/2017)... ... July 22, 2017 , ... ... with the majority of patients not requiring pain medication after three months post-operatively. ... Medicine’s Annual Meeting today in Toronto, Ontario, Canada found that those patients ...
Breaking Medicine News(10 mins):
(Date:7/21/2017)... , July 21, 2017  Endo International plc ... completing a comprehensive review of its manufacturing network, the ... and distribution facilities in Huntsville, Alabama ... take place over the next 12 to 18 months. ... impacted by declining volumes of commoditized products and these ...
(Date:7/19/2017)... , July 19, 2017  Mako Medical Laboratories ... and the Military Family Assistance Fund (MFA) to bring ... to visit with their families one last time ... to coordinate the travel and logistics needed for these ... deployed soldiers and their families. We just wish we ...
(Date:7/14/2017)... plc (NASDAQ: ENDP ) will announce its second-quarter 2017 ... management team will host a conference call and webcast before the ... The dial-in number to access the call is U.S./ Canada ... 45397076. Please dial in 10 minutes prior to the scheduled start ... the call will be available from August 8, 2017 at 11:30 ...
Breaking Medicine Technology: