Navigation Links
Cold Spring Harbor Protocols features tissue culture methods for immune cells and neurons
Date:12/2/2008

COLD SPRING HARBOR, N.Y. (Tues., Dec. 2, 2008) The complexity of vital organ systems makes them difficult to study in living organisms. Tissue culture methods for specific cell types allow researchers to break these systems down into component parts that can be readily manipulated and observed. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc12_08.dtl) features two articles detailing experimental culture methods for cells from the immune system and the nervous system.

"Bone Marrow-Derived Macrophages (BMM): Isolation and Applications," from Bo Porse and colleagues at the University of Copenhagen (http://www.bric.ku.dk/research/porse_group/), describes the derivation and growth of bone marrow-derived macrophages. Macrophages are specialized cells that carry out numerous tasks in the immune system such as phagocytosis, antigen presentation, cytokine production and migration. Cultured cells can be used to study these functions and to perform assays for gene expression, gene function and interaction with microbes. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/13/pdb.prot5080).

Primary cultures of granule neurons from the cerebellum provide an excellent model system for molecular and cell biological studies of neuronal development and function. Many fundamental insights into the processes of neuronal apoptosis, migration, and differentiation in the mammalian central nervous system have come from investigating granule neurons in vitro. Azad Bonni's laboratory at Harvard University (http://pathology.hms.harvard.edu/labs/bonni/index.html) provides "Cultures of Cerebellar Granule Neurons," a protocol for isolating and culturing these cells. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/13/pdb.prot5107).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related medicine news :

1. Cold Spring Harbor science teams identify 13 new tumor-suppressor genes in liver cancer
2. Canola Oil Consumed During Pregnancy Lowers Breast Cancer Risk for Offspring
3. Susan G. Komen for the Cure(R) Hosts Inaugural Illinois State Summit in Springfield
4. This Thanksgiving Season, Lifespring Health is Helping You Donate While You Shop
5. Springer launches Cognitive Computation
6. WellSpring Pharmaceutical Corp. Names Wendy Shusko as New Chief Operating Officer
7. Springer adds Journal of Neurodevelopmental Disorders to biomedical journal portfolio
8. WellSpring Pharmaceutical Corp. Acquires Consumer Product Brands from McNeil Consumer Healthcare -- Deal Enhances US and Canadian Pharmaceutical Business
9. The Health Insurance Advocate Radio Show Expands to Springfield, Missouri, and Reaches a New All-Time Ratings High for Female Listenership
10. Insomnia in parents can result in sleep problems, suicidal behavior among their offspring
11. HMS Holdings Corp. to Present at Stephens 2008 Spring Investment Conference
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/25/2017)... ... September 25, 2017 , ... The trend-spotters, tastemakers, and healthy lifestyle ... organic coconut-milk based super herb drinks, with the new NEXTY Gold Award at the ... year for the NEXTY Gold, which is reserved for just those companies that hit ...
(Date:9/25/2017)... ... September 25, 2017 , ... ... September 28, 2017 to deliver a powerful message of teamwork, unity and collaboration ... Feature film writer/director Robert Adetuyi (Beat the World, Stomp the Yard) and Grammy-nominated ...
(Date:9/24/2017)... ... September 23, 2017 , ... Throughout the United States and the world, clinicians ... physicians, nurses and other providers work to give the best care possible to patients ... But U.S. Senators today are threatening to tie our hands in carrying out that ...
(Date:9/24/2017)... ... ... I”, a young boy was walking home and feeling down about himself. He didn’t think ... new friend says he can do whatever he wants to do if he puts his mind ... he is. God created him with special talents and gifts. We see his internal dialogue ...
(Date:9/23/2017)... ... , ... Silicon Valley Hair Institute, the leading hair transplant center in the ... the ARTAS® hair transplant system and the younger demographic. As many younger people suffer ... methods of hair restoration. , “It can be emotionally difficult for young men and ...
Breaking Medicine News(10 mins):
(Date:9/7/2017)... BOTHELL, Wash. , Sept. 7, 2017   BioLife ... developer, manufacturer and marketer of proprietary clinical grade cell and ... media ("BioLife"), announces that Mike Rice , President and ... Rodman & Renshaw 19 th ... 3:25 p.m. Eastern time (12:25 p.m. Pacific time). The conference ...
(Date:9/6/2017)... Sept. 6, 2017  Medical professionals are ... skills while treating their patients. Medical simulations ... without involving patients. Simulation provides a safe ... carry out procedures, refine techniques and build ... of new technology, such as augmented reality, ...
(Date:9/6/2017)... 6, 2017 NeuroRx, a clinical stage biopharma company ... Behavior (ASIB), has been granted Fast Track status by the ... NRX-100 (ketamine HCl) followed by NRX-101 (D-cycloserine + lurasidone). The ... of this sequential therapy targeting patients who are admitted to ... ...
Breaking Medicine Technology: