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Cold Spring Harbor Protocols features innovative methods for embryology research
Date:12/3/2007

d chicken) embryos using a method called electroporation. The DNA vector contains a gene of interest attached to a fluorescent marker, which allows the researchers to track the fluorescently labeled cells using imaging techniques. Lansfords group uses this method to investigate mechanisms of brain and heart development in birds.

In Lansfords protocol, the DNA is electroporated into shell-less bird eggs. There are several advantages to using shell-less eggs: each embryo can be more accurately positioned during electroporation, and the researchers can ensure that all embryos used in the experiment are at the same stage in development. The protocol is freely available here: www.cshprotocols.org/cgi/content/full/2007/24/pdb.prot4894.


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Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

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