Navigation Links
Cold Spring Harbor Protocols features innovative methods for embryology research
Date:12/3/2007

COLD SPRING HARBOR, N.Y. (Mon., Dec. 3, 2007) Two methods that permit scientists to examine critical stages in early embryogenesis are featured in this months release of Cold Spring Harbor Protocols. The methods, which are freely accessible online (www.cshprotocols.org), describe how to fluorescently tag cells in very young embryos. These cell-tagging techniques, combined with sophisticated imaging methods, permit scientists to visualize even subtle movements of individual cells in the embryos, as they morph, divide, and migrate.

The first protocol, available at www.cshprotocols.org/cgi/content/full/2007/24/pdb.prot4915, describes a step-by-step approach to label specific cells of live mouse embryos using fluorescent dyes called carbocyanine dyes. Carbocyanine dyes are ideal for this purpose because they can be used on living embryos. After labeling, the embryos are imaged to reveal the precisely coordinated patterns of cell movements as the embryo develops.

The protocol was contributed by Dr. Patrick Tams group from the University of Sydney, Australia (www.medfac.usyd.edu.au/people/academics/profiles/ppltam.php). His lab uses these techniques to investigate the timing and patterning of cell movements during gastrulation, which is a critical stage in early embryonic development. Also, by performing this procedure in embryos that are both normal and genetically mutated, they can better understand the functions of specific genes involved in gastrulation.

The second featured protocol is from Dr. Rusty Lansfords lab at Caltech (quad.bic.caltech.edu/~fraserlab/people/lansford/). It describes how to insert a DNA vector into very young bird (quail and chicken) embryos using a method called electroporation. The DNA vector contains a gene of interest attached to a fluorescent marker, which allows the researchers to track the fluorescently labeled cells using imaging techniques. Lansfords group uses this method to investigate mechanisms of brain and heart development in birds.

In Lansfords protocol, the DNA is electroporated into shell-less bird eggs. There are several advantages to using shell-less eggs: each embryo can be more accurately positioned during electroporation, and the researchers can ensure that all embryos used in the experiment are at the same stage in development. The protocol is freely available here: www.cshprotocols.org/cgi/content/full/2007/24/pdb.prot4894.


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Related medicine news :

1. Eating junk food whilst pregnant and breastfeeding may lead to obese offspring
2. Adult offspring of parents with PTSD have lower cortisol levels
3. A classic method for modeling skin cancer is featured in Cold Spring Harbor Protocols
4. Partnership for a Drug-Free America and Colorado Springs Police Department Introduce Methamphetamine Prevention Program to Colorado Law Enforcement
5. Parents PTSD May Boost Stress in Offspring
6. International Consulting Firm Health Fitness Dynamics Hired to Oversee Springbrook Inns Planned New Spa
7. COLORBLENDS to Donate Portion of Sales of Spring Forward Daffodil Blend to the Christopher and Dana Reeve Foundation
8. Springer adds Advances in Therapy to journals portfolio
9. Lifespring Health Promotes Health and Fitness by Sponsoring Silicon Valley Marathon on November 3rd and 4th
10. Announcing Always Best Care Senior Services Expansion into Colorado Springs, Colorado
11. Cold Spring Harbor scientists devise novel, low-cost method of sifting genomes high-value regions
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/22/2017)... ... February 22, 2017 , ... ... in recent years. The technology is so cutting edge, in fact, the U.S. ... for stem cell procedures. However, successful patient outcomes in certain clinical stem cell ...
(Date:2/22/2017)... (PRWEB) , ... February 22, 2017 , ... ... its certification exam and eligibility requirements effective with the April 2017 testing period. ... 1995. , “The Care Manager Certified (CMC) exam is periodically re-calibrated to ...
(Date:2/22/2017)... Dallas, TX (PRWEB) , ... ... ... HCC Risk Adjustment solution leverages advanced data analytics to accurately understand each ... ensure proper reimbursement for a given population. This new solution helps transform ...
(Date:2/22/2017)... ... ... Doctors on Liens is continuing its steady expansion of medical specialties throughout ... North Valley Eye Medical Group in Mission Hills . Eye injuries and vision ... cases. These injuries have a major impact on the quality of life of ...
(Date:2/22/2017)... ... February 22, 2017 , ... Using the power of the ... simple online checklist. Over a period of just 24 months, thousands of individuals interested ... online checklist called T.A.D. , “The internet is not getting quieter. In fact it’s ...
Breaking Medicine News(10 mins):
(Date:2/22/2017)... 2017 IRIDEX Corporation (Nasdaq: IRIX ... for the fourth quarter and 2016 after the close ... management team will host a corresponding conference call beginning ... Investors interested in listening to the conference call ... callers or (703) 326-3030 for international callers, using conference ...
(Date:2/22/2017)... , Feb. 22, 2017 Summary ... infarction partnering deals and agreements entered into by ... full report: http://www.reportlinker.com/p03605670-summary/view-report.html Description The ... understanding and access to partnering deals and agreements ... - Trends in partnering deals ...
(Date:2/22/2017)... , Feb. 22, 2017 Merrimack Pharmaceuticals, Inc. ... will host its Fourth Quarter 2016 investor conference call and ... The call will cover an update on ... quarter and full year 2016 financial results. A press release ... be issued the morning of Wednesday, March 1. Investors and ...
Breaking Medicine Technology: